Paprapach Wongdontree

Two temperature-dependent membrane fluidity regimes in Gram-positive bacteria https://www.biorxiv.org/content/10.64898/2026.01.22.701129v1

Exploiting the bacterial DNA damage response as an alternative antimicrobial at Newcastle University on FindAPhD.com PhD Project - Exploiting the bacterial DNA damage response as an alternative antimicrobial at Newcastle University, listed on FindAPhD.com

E. coli is the leading bacterium causing death from AMR infections worldwide. If you'd like to explore approaches to exploit the DNA damage response as alternative antimicrobial, please apply to the following PhD studentship available in my lab starting October 2026: www.findaphd.com/phds/project...

MPKaDB: A pKa Database for Exploring pH Dependence in Membrane Proteins https://www.biorxiv.org/content/10.1101/2025.11.14.688495v1

A two-component system signaling hub controls enterococcal membrane remodeling in response to daptomycin https://www.biorxiv.org/content/10.1101/2025.11.16.688641v1

BioPrediction-PPI: Simplifying the Prediction of Protein-Protein Interactions through Artificial Intelligence https://www.biorxiv.org/content/10.1101/2025.11.16.688401v1

🔊 New story!

This GlmR (of hope) article has it all. Cell shape, cytokinesis, c-di-AMP, and catalysis - the famous 4 Cs 💎?

There is also antibiotic resistance, phosphorylation, acetylation, and a (cool) model to explain what shapes Bacillus cells.

Excellent group effort. Congrats team!

#Microsky

Recruitment of the outer-membrane lipoprotein DolP to thedivision site via cardiolipin-mediated diffusion-stateswitching https://www.biorxiv.org/content/10.1101/2025.10.29.685289v1

Molecular dissection of Class A PBP function uncovers novel features of the non-canonical Clostridioides difficile divisome complex Author summary Bacterial cell division is an ancient and essential process, but our molecular understanding of this process is primarily based on studies in a select few model systems. Recent work fou...

Excited to share my latest postdoctoral work in the Shen Lab at Tufts! In this study, we follow up on an exciting finding by former grad student @shailab.bsky.social that C diff uses its Class A PBP (PBP1) to drive cell division (unlike previously studied bacteria)! journals.plos.org/plosgenetics...

Postdoctoral researcher in molecular ecology - Uppsala University Postdoctoral researcher in molecular ecology, Department of Ecology and Genetics, Uppsala University

📢 We have an open position for a postdoc to join my lab. It's a great position @animecol-uu.bsky.social, fully salaried for 2.5 years with all benefits.

www.uu.se/en/about-uu/...

The project is about transmission patterns of bacteria and antimicrobial resistance (AMR) in aquatic insects. 🧬🦠 (1/3)

Mechanical stress in the S. aureus cell wall during the cell cycle. (A) As the cell cycle progresses, stress at the equator decreases due to growth of the septum while the stress remains unchanged at the pole. The stress distributions shown here were calculated for MSSA with cell volume 1.22 µm3, thickness 20 nm, turgor pressure 2 MPa, and septum relative stiffness 1.2. (B) Graphical representation of the changes in circumferential stress at the cell pole and equator as septum synthesis progresses. Here, septum progression is defined as (a − b)/a, where a is the cell radius and b is the septum aperture size, which decreases as the septum is formed (note that a and b are defined for the unpressurized cell; see Supplementary Information, Section II).

#MicrobiologyMonday: Staphylococcus aureus divides by building a septum and then splitting into 2 daughter cells. But how is septum formation coordinated with cell scission? Research in #mBio suggests cell wall mechanical stress could play a role: asm.social/2Eq

Identification of radical SAM enzymes responsible for the methylation and desaturation of archaeal lipids and an AttH hydratase mediating hydroxy-GDGT biosynthesis https://www.biorxiv.org/content/10.1101/2025.10.20.683597v1

Chromosome segregation dynamics during the cell cycle of Staphylococcus aureus - Nature Communications Our understanding of chromosome organization and dynamics in spherical bacteria, such as Staphylococcus aureus, remains limited. Here, the authors show that chromosome replication and cell division cycles are not synchronized in S. aureus, with cells exhibiting two segregated origins of replication at the start of the cell cycle.

Chromosome replication and cell division cycles are not synchronized in Staphylococcus aureus, with cells exhibiting two segregated origins of replication at the start of the cell cycle

#bacteria #microbiology

🦠🔬🤖🧑‍💻 #mAIcrobe is out! With @pinholab.bsky.social's lab, we launched an open-source framework for high-throughput bacterial image analysis. By rockstars A. Brito & B. Saraiva et al, making #DeepLearning for phenotyping accessible! Easy to use, plus model training

📜 www.biorxiv.org/content/10.1...

Using super-resolution microscopy to investigate the mechanistic principles of bacterial cell wall remodelling at University of Warwick on FindAPhD.com PhD Project - Using super-resolution microscopy to investigate the mechanistic principles of bacterial cell wall remodelling at University of Warwick, listed on FindAPhD.com

PhD project available in my lab. Email me with CV if interested
www.findaphd.com/phds/project...
#microsky #bioimaging

We’re excited to announce the incredible speaker line-up for the Prokaryotic Cell Biology Conference! Join the leading researchers as they explore the latest advances in Nanoimaging, Cell Growth and Cellular Structures.
Join our speakers & register today! ➡️https://bit.ly/47rtkbw
#ProkaryoticCellBio

Peptidoglycan recycling is critical for cell division, cell wall integrity and β-lactam resistance in Caulobacter crescentus https://www.biorxiv.org/content/10.1101/2025.10.15.682734v1

Structural basis for MurJ inhibition by phage lysis protein SglPP7 suggesting convergence https://www.biorxiv.org/content/10.1101/2025.10.15.682725v1

A Protein Antibiotic Inhibits the BAM Complex to Kill Without Cell Entry Many antibiotics are ineffective against Gram-negative pathogens such as Pseudomonas aeruginosa because they cannot penetrate the bacterial outer membrane. Here, we show that protein antibiotics calle...

The outer membrane of Gram-negative bacteria blocks many antibiotics. Our latest work reveals that L-type pyocins bypass this barrier by inactivating the BAM complex, killing Pseudomonas aeruginosa without entering the cell, providing a new blueprint for beating antibiotic resistance.

Comprehensive genetic interaction analysis of the Bacillus subtilis envelope using double-CRISPRi Koo et al. apply genome-scale double-CRISPRi to map cell envelope gene interactions in Bacillus subtilis, revealing >1,000 genetic interactions and uncovering gene networks in envelope biogenesis and ...

Also see the accompanying paper from Carol Gross' lab that used a similar approach for Bacillus subtilis: www.cell.com/cell-systems...

How nutrient starvation impacts the gut microbiome - Nature Microbiology In this Perspective, Estrela and Huang discuss nutrient starvation in the context of the gut microbiome, outlining what is known and highlighting key questions for future research to address knowledge gaps.

#Perspective

Estrela and Huang discuss nutrient starvation in the context of the gut microbiome, outlining what is known and highlighting key questions for future research to address knowledge gaps.

#MicroSky #MicrobiomeSky 🦠

www.nature.com/articles/s41...

Peptidoglycan remodelling improves salt resilience of Zymomonas mobilis https://www.biorxiv.org/content/10.1101/2025.10.04.680478v1

A photo of a Nobel medal

BREAKING: The Nobel Prize in Physiology or Medicine has been awarded jointly to Mary E. Brunkow, Fred Ramsdell, and Shimon Sakaguchi "for their discoveries concerning peripheral immune tolerance"

Stay tuned for more.
#NobelPrize

Inorganic sulfate is critical for Mycobacterium tuberculosis lung tissue colonization and redox balance | PNAS Tuberculosis remains the deadliest infectious disease caused by a single pathogen, highlighting the urgent need for novel therapies. A deeper under...

Excited to share Wendy Le Mouëllic’s PhD work, now published in @pnas.org!
It reveals that M. tuberculosis depends on inorganic sulfate import to survive inside host cells—fueling essential processes such as redox balance and stress resistance.
Huge congrats to Wendy & colleagues!
shorturl.at/WbFQC

Architecture of a peptidoglycan peptidase complex involved in morphological transition in Helicobacter pylori. https://www.biorxiv.org/content/10.1101/2025.08.29.672660v1

Divisome dynamics in intracellular UPEC cells during infection. Left: Representative confocal image of intracellular UTI89 expressing a division marker (FtsZ-mCitrine; pseudo coloured green) with mCherry (pseudo coloured magenta) as cytoplasmic marker, bladder cell membranes shown in blue (CellBrite Steady Membrane 405) and nuclei shown in gold (NucSpot Live Cell 650). Right: Still images of a time-lapse sequence showing FtsZ-mCitrine (pseudo coloured green) driven coccobacilli divisions imaged using HiLo illumination.

Uropathogenic #Ecoli #UPEC proliferate as round coccoid cells during intracellular infection; @alaskapokhrel.bsky.social @bill-lab.bsky.social &co show that accelerated division via FtsZ constriction drives this, the coccobacillus form persisting for generations @plosbiology.org 🧪 plos.io/4gdx97i

Conservation of sporulation genes and a transmembrane-containing Spo0B variant in Paenibacillus Sporulation is a strategy employed by many bacteria to survive harsh environmental conditions. The genus Paenibacillus includes spore-forming species notorious for spoiling pasteurized dairy products ...

Here we show that >90% of Paenibacillus encode a unique Spo0B with a transmembrane domain! TM domain helps it interact with its phosphorelay partners. Congrats to @isabellalin.bsky.social who left for grad school this week and her mentor @cassidyprints.bsky.social! www.biorxiv.org/content/10.1...

MCDB Opens Search for Faculty | U-M LSA Molecular, Cellular, and Developmental Biology (MCDB) MCDB welcomes applications at the Assistant Professor level from outstanding biological scientists in all areas of physiology and neuroscience, spanning molecular, cellular, systems, and organismal le...

My MCDB dept is accepting applications for a faculty position at the Assistant Professor level!

Applications Due by October 15, 2025

We welcome applications from all areas of physiology and neuroscience, spanning molecular, cellular, systems, and organismal levels.

lsa.umich.edu/mcdb/news-ev...

Staphylococcus aureus exploits lipoic acid salvage to combat host oxidative stress Acosta et al. find that ADP-ribosylation of the lipoic acid carrier protein GcvH-L promotes Staphylococcus aureus resistance to oxidative burst and facilitates infection. ADP-ribosylation leads to a s...

Happy to share Iván’s first-author pub from the lab! www.cell.com/cell-reports...

Good opportunity here to work with a super talented new PI.

"What makes S. aureus such a difficult vaccination candidate?" Here are some answers: