International Society for Protein Termini (ISPT)

Manipulating plant oxygen sensing through NCO substitution reveals trade-offs between growth and flooding tolerance The oxygen-dependent degradation of Ethylene Response Factors VII (ERFVIIs) through the N-degron pathway is central to regulating the transcriptional responses to hypoxia in vascular plants. Plant Cys...

Can we modulate plant oxygen sensing to enhance flood resilience?🌿🔧

www.biorxiv.org/content/10.1...

This is one of two preprints out this week from the @syno2xis.bsky.social and @emilyflashman.bsky.social labs, two angles on how PCOs shape plant flood resilience. Don’t miss the full story!

Expression and purification of ribosome associated factors: Methionine aminopeptidases and N-terminal acetyltransferases | Methods in Enzymology | Marius Klein and Irmgard Sinning @uniheidelberg.bsky.social @ispt-proteinterm.bsky.social www.sciencedirect.com/science/arti...

How to express, purify and assess the activity of the ATE1 arginyltransferase | Methods in Enzymology | Yi Zhang lab @ispt-proteinterm.bsky.social www.sciencedirect.com/science/arti...

Method to determine the identity and modification state of plastid precursor protein N-termini in the cytosol by combining protoplast protein import assays with targeted mass spectrometry | Methods in Enzymology | Baginsky lab. @ispt-proteinterm.bsky.social www.sciencedirect.com/science/arti...

Bidirectional control of a metabolic transition by the GID ubiquitin ligase The GID/CTLH ubiquitin ligase is a multisubunit E3 conserved across eukaryotes. GID/CTLH has been implicated in a variety of processes, including metabolic regulation, cell proliferation, embryonic de...

Gid11 is a substrate receptor of the GID multisubunit E3 ligase during a metabolic switch. Specific degradation of proteins linked to glycolysis via their N-terminal threonine lacking N-terminal acetylation. #N-degron Khmelinskii lab @ispt-proteinterm.bsky.social
www.biorxiv.org/content/10.1...

Manipulating plant oxygen sensing through NCO substitution reveals trade-offs between growth and flooding tolerance The oxygen-dependent degradation of Ethylene Response Factors VII (ERFVIIs) through the N-degron pathway is central to regulating the transcriptional responses to hypoxia in vascular plants. Plant Cys...

Manipulating plant oxygen sensing through NCO substitution reveals trade-offs between growth and flooding tolerance. Perri et al. - another preprint from the Licausi and Flashman labs. @monicaperri1.bsky.social @emilyflashman.bsky.social @syno2xis.bsky.social www.biorxiv.org/content/10.1...

Engineering reduced activity in the oxygen-sensing Arabidopsis thaliana plant cysteine oxidase 4 enzyme results in improved flood resilience Plant cysteine oxidases (PCOs) are oxygen-sensing enzymes that play an important role in plant responses to low oxygen (hypoxia). PCO-catalysed dioxygenation of the N-terminal Cys of substrates, inclu...

Engineering reduced activity in the oxygen-sensing Arabidopsis thaliana plant cysteine oxidase 4 enzyme results in improved flood resilience. By Dirr et al. Licausi and Flashman labs @syno2xis.bsky.social @emilyflashman.bsky.social www.biorxiv.org/content/10.1...

Excited to announce our new methods paper in Methods in Enzymology!

We share our pipeline for building high-diversity DNA libraries & exploring protein stability in E. coli.

Free access until Sept 4: authors.elsevier.com/a/1lRkeHRzCX...
Updated, companion preprint: www.biorxiv.org/content/10.1...

New paper out in Mol Cell, where we show how N-myristolytransferases lipidate proteins on the translating ribosome!
Continuing our successful collaboration with DeuerlingLab @uni-konstanz.de & Shu-ou Chan @caltech.edu
@snsf.ch @ethz.ch
www.cell.com/molecular-ce...

DEGRONOPEDIA - a web server for identifying and predicting protein degradation signals across eukaryotic proteomes. Detailed guide by Natalia Szulc @lumirare.com and Wojciech Pokrzywa @wpokrzywa.bsky.social in Methods in Enzymology. @ispt-proteinterm.bsky.social www.sciencedirect.com/science/arti...

How to use bacteria for arginylation assessment. Co-expression of ATE1 and substrate outlined by Yi Zhang and colleagues in Methods in Enzymology. @ispt-proteinterm.bsky.social www.sciencedirect.com/science/arti...

How to detect protein N-terminal arginylation - a key step of protein degradation via the Arg/N-degron pathway. By Jasper Eising and @ndissmeyer.bsky.social in Methods in Enzymology. @ispt-proteinterm.bsky.social www.sciencedirect.com/science/arti...

How to assess protein stability and N-degrons via the Ubiquitin reference technique. From the Protein Metabolism Lab (Yong Tae Kwon) at SNU Seoul ups.snu.ac.kr in Methods in Enzymology @ispt-proteinterm.bsky.social www.sciencedirect.com/science/arti...

This cover highlights the article Early coordination of cell migration and cardiac fate determination during mammalian gastrulation by Shayma Abukar, Kenzo Ivanovitch and colleagues. Using long-term live imaging of whole mouse embryos with light-sheet microscopy, this study tracks single cells to map how mesodermal cells coordinate their migration and fate decisions during gastrulation to drive early heart formation. The cover image shows the cardiac crescent (blue) and a late T/Bra-lineage-positive mesodermal subpopulation (red) in a DAPI-stained E7.5 mouse embryo (white). The image illustrates that late mesodermal cells contributing to the atria do not populate the cardiac crescent. Scientific image by Kenzo Ivanovitch

Issue 12:
Commentary: in vivo metabolomics in the cancer clinic
Review: oxygen-sulfur interdependence
ADP-ribosylation in M.tuberculosis
Microprotein promoting BAT thermogenesis
Cover by @kenzoivanovitch.bsky.social and colleagues
www.embopress.org/toc/14602075...

Celebrating 35 years of Trends in Cell Biology and introducing our special issue on ribosomal dynamics and cellular homeostasis As Trends in Cell Biology marks its 35th anniversary, we pause to reflect on the incredible journey this journal has taken since its launch. Over three and a half decades, Trends in Cell Biology has chronicled the evolving landscape of cellular research, providing a platform for pioneering insights that have shaped our understanding of fundamental biological processes. From early explorations of cellular architecture to today’s sophisticated molecular and systemic analyses, the journal has continually embraced scientific innovation and fostered a vibrant community of researchers.

Celebrating 35 years of Trends in Cell Biology and introducing our special issue on ribosomal dynamics and cellular homeostasis

GID4 recognition of Pro/N-degron peptides: Conformational selection and induced fit The N-degron pathway is essential for protein quality control and cellular homeostasis. GID4, a subunit of the GID ubiquitin ligase, is the main recog…

GID4 recognition of Pro/N-degron peptides: Conformational selection and induced fit www.sciencedirect.com/science/arti...

Ribosomal expansion segment contributes to translation fidelity via N-terminal processing of ribosomal proteins academic.oup.com/nar/article/...

ERFVIIs as transducers of oxygen-sensing in the evolution of land plant response to hypoxia Short summary:This study investigates the evolution of the low-oxygen response in land plants mediated by group VII Ethylene Response Factors (ERFVIIs). We identified conserved transcriptional signatu...

How did oxygen-sensing evolve in plants?
In collaboration with @syno2xis.bsky.social Great work from @lauradc.bsky.social et. al. #plantscience @leverhulme.ac.uk
“ERFVIIs as transducers of oxygen-sensing in the evolution of land plant response to hypoxia”
www.cell.com/molecular-pl...

Protein acetylation as a novel post-translational modification of plant peroxisomal proteins We summarize research data newly demonstrating that plant peroxisomal proteins are modified co- and post-translationally by acetylation, and we predict the

Protein acetylation as a novel post-translational modification of plant peroxisomal proteins. @ispt-proteinterm.bsky.social academic.oup.com/jxb/article-...

Combinatorial mutagenesis of N-terminal sequences reveals unexpected and expanded stability determinants of the Escherichia coli N-degron pathway Although it is known that penultimate N-terminal residues can influence protein stability by the N-degron pathway, there has not been a comprehensive effort to document nor predict these effects for n...

Protein degradation in E. coli via N-terminal degradation signals (degrons) defined by the @kunjapur.bsky.social lab. @ispt-proteinterm.bsky.social www.biorxiv.org/content/10.1...

An mRNA-display derived cyclic peptide scaffold reveals the substrate binding interactions of an N-terminal cysteine oxidase - Nature Communications This work identified cyclic peptide inhibitors of a mammalian N-terminal cysteine oxidase, ADO, through mRNA display, one of which was used as a scaffold to graft substrate moieties, allowing key inte...

Oxygen sensors structurally defined. The substrate binding of N-terminal cysteine oxidases uncovered by the White lab @drmdwhite.bsky.social in @natcomms.nature.com www.nature.com/articles/s41...

OsHYPK/NatA-mediated N-terminal acetylation regulates the homeostasis of NLR immune protein to fine-tune rice immune responses and growth Huang et al. identify that an NLR protein RPM1-L1, which is N-terminally acetylated by OsHYPK/NatA, is involved in regulating blast resistance in rice, showing an OsHYPK-NTA-RPM1-L1 model that fine-tu...

Protein N-terminal acetylation regulates the homeostasis of NLR immune protein to fine-tune rice immune responses and growth. @ispt-proteinterm.bsky.social @cp-cellreports.bsky.social www.cell.com/cell-reports...

Understanding brain calcification via N-terminal acetylation at the Golgi apparatus Primary familial brain calcification (PFBC) is a genetically heterogeneous disorder marked by bilateral calcium deposition in the brain parenchyma. Siggerv

How can defective NAA60-mediated N-terminal acetylation cause brain calcification? New perspectives from @henrietteaksnes.bsky.social lab in Brain @oxfordacademic.bsky.social #NAA60 @ispt-proteinterm.bsky.social doi.org/10.1093/brai...

Hypoxia‐activated fluorescent probes as markers of oxygen levels in plant cells and tissues Low oxygen signalling in plants is important in development and stress responses. Measurement of oxygen levels in plant cells and tissues is hampered by a lack of chemical tools with which to reliab...

And now formally published, a chemical probe that can detect hypoxia in plants - congratulations to all authors! nph.onlinelibrary.wiley.com/doi/full/10....

Looking forward to the @ukbspr.bsky.social meeting in July!

DPP8/9 processing of human AK2 unmasks an IAP binding motif | EMBO reports imageimageA novel regulatory mechanism controls AK2 stability in the cytosol, linking mitochondrial protein processing to proteasomal degradation. Cytosolic degradation of adenylate kinase 2 (AK2) and potentially 129 additional proteins is ...

Protein degradation mediated by DPP8/DPP9 peptidases by unmasking N-degrons recognized by Inhibitors of apoptosis (IAPs) E3 ligases. By Bange and Riemer labs. @emboreports.org www.embopress.org/doi/full/10....

Cardiological Manifestations in Males and Females Affected by NAA10‐Related Disease We report a family with two affected brothers presenting hypertrophic cardiomyopathy, prolonged QT interval, and intellectual disability who, after a dozen years of inconclusive genetic testing, were...

Cardiological Manifestations in Males and Females Affected by NAA10-Related Disease. Impact of Protein N-terminal acetylation. @ispt-proteinterm.bsky.social
onlinelibrary.wiley.com/doi/10.1002/...

Cardiac manifestations and their recommended clinical cardiac management in Ogden syndrome. Pathogenic NAA10 variants and impaired protein N-terminal acetylation. rdcu.be/ejP0m

Dysregulation of N-terminal acetylation causes cardiac arrhythmia and cardiomyopathy - Nature Communications N-terminal acetylation dysregulation in the heart causes severe arrhythmia and cardiomyopathy. The authors show that stem cell models demonstrate ion channel trafficking defects and sarcomeric disarra...

Dysregulation of N-terminal acetylation causes cardiac arrhythmia and cardiomyopathy. #NAA10 @ispt-proteinterm.bsky.social www.nature.com/articles/s41...

In-depth plasma N-glycoproteome profiling using narrow-window data-independent acquisition on the Orbitrap Astral mass spectrometer Nature Communications - While DIA has advanced standard proteomics, its application in glycoproteomics has remained limited. Here, the authors present an optimized narrow-window DIA method for...

rdcu.be/ehinQ Read how a $1,- piece of cotton makes the difference for Shelley Jager enabling in-depth plasma N-glycoproteome profiling