International Society for Protein Termini (ISPT)

NAA60 facilitates LRRC8A- and LRRC8D-mediated platinum drug uptake - Communications Biology Genome-wide CRISPR/Cas9 screening identifies the N-terminal acetyltransferase NAA60 to facilitate cis- and carboplatin drug sensitivity, most likely by neutralizing Nt acetylation of the LRRC8A/D VRAC subunits regulating substrate permeability.

Uptake of anti-cancer drugs depends on N-terminally acetylated anion channels | @ispt-proteinterm.bsky.social www.nature.com/articles/s42...

A Cell-Potent Bisubstrate Inhibitor to Probe NatD Acetyltransferase Activity N-terminal acetyltransferase D (NatD) is a highly selective enzyme that acetylates the α-N-terminal amine of histones H4 and H2A, which share the SGRGK motif. Elevated NatD expression has been observe...

A Cell-Potent Bisubstrate Inhibitor to Probe NatD Acetyltransferase Activity | The inhibitor effectively reduced cellular Nα-acetylation on histone H4, leading to reduced migration in lung cancer cells | @acs.org ACS Chemical Biology @ispt-proteinterm.bsky.social pubs.acs.org/doi/10.1021/...

Protein N-terminal acetylation regulates the growth of rice plants | #NAA30 #NatC #rice #acetylation www.sciencedirect.com/science/arti...

Two new volumes of Methods in Enzymology with 29 chapters/papers on Protein termini methods and reviews are now out! Thx to all authors!🙌 @ispt-proteinterm.bsky.social #N-degron #prenylation #myristoylation #formylation #arginylation #oxidation #acetylation www.sciencedirect.com/bookseries/m...

Developing leaves form a spatiotemporal oxygen gradient that is sensed through the oxygen sensing machinery and directs morphogenesis | New preprint from @daanweits.bsky.social and @syno2xis.bsky.social labs #N-degron #Cysteine #hypoxia @ispt-proteinterm.bsky.social www.biorxiv.org/content/10.1...

Anatomy and habitat shape the oxygen sensing machinery of angiosperms Hypoxia sensing via the Cys/Arg branch of the N-degron pathway (Cys-NDP) is central for flooding responses in plants, yet how evolutionary and ecological factors have shaped the core oxygen sensing me...

Anatomy and habitat shape the oxygen sensing machinery of flowering plants | new preprint from Licausi lab @syno2xis.bsky.social #hypoxia #N-degron @ispt-proteinterm.bsky.social www.biorxiv.org/content/10.1...

The generation and consequences of N-terminal proteoform diversity N-terminal proteoforms are protein variants with altered N termini that arise from RNA-driven processes, such as alternative promoter usage, splicing,…

New review on the generation and consequences of N-terminal proteoform diversity from Evan Morrison and Olivia Rissland | #N-degron #RNA @cp-cellreports.bsky.social www.sciencedirect.com/science/arti...

Targeted degradation of pathogenic TDP-43 proteins in amyotrophic lateral sclerosis using the AUTOTAC platform Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease characterized by the progressive loss of motor neurons and the cytoplasmic aggregation of misfolded proteins in the spinal cord...

Targeted degradation of pathogenic TDP-43 proteins in amyotrophic lateral sclerosis using the AUTOTAC platform | Yong Tae Kwon lab | #N-degron www.biorxiv.org/content/10.1...

A synthetic ERFVII-dependent circuit in yeast sheds light on the regulation of early hypoxic responses of plants Plants face hypoxic conditions either chronically, as particular tissues are characterized by fluctuating or stable low oxygen levels, or acutely, when flooded. In vascular plants, transcriptional ada...

A synthetic ERFVII-dependent circuit in yeast sheds light on the regulation of early hypoxic responses of plants | Preprint from @mikellavi.bsky.social @beagiuntoli.bsky.social @syno2xis.bsky.social #N-degron #hypoxia www.biorxiv.org/content/10.1...

Want to know how lipidation of some nascent chains takes place by NMT2-- Check our latest work on how NAC couples Protein Synthesis with Nascent Polypeptide Myristoylation on the Ribosome out today @embojournal.org‬: www.embopress.org/doi/full/10....

Nitric oxide promotes cysteine N-degron proteolysis through control of oxygen availability | PNAS Selected proteins containing an N-terminal cysteine (Nt-Cys) are subjected to rapid, O2-dependent proteolysis via the Cys/Arg-branch of the N-degro...

Nitric oxide promotes cysteine N-degron proteolysis through control of oxygen availability | Important finding by Kim, Tian, Ratcliffe and Keeley @ox.ac.uk @ispt-proteinterm.bsky.social #N-degron www.pnas.org/doi/10.1073/...

Gene Editing of a Susceptibility LncRNA Enhances Broad‐Spectrum Disease Resistance in Rice without Developmental Trade‐Offs Editing susceptibility lncRNAs offers a strategy to enhance disease resistance without yield loss. RESIS, a domestication-acquired, pathogen-induced lncRNA, promotes protein N-terminal acetylation th...

Disease resistance in rice steered by a long non-coding RNA via protein N-terminal acetylation | @ispt-proteinterm.bsky.social advanced.onlinelibrary.wiley.com/doi/10.1002/...

Fine mapping and prediction of a candidate gene for wrinkled rind in melon (Cucumis melo L.) - Theoretical and Applied Genetics Key message The Cmwr locus was fine mapped to chromosome 2 in wrinkled-rind melon, and MELO3C010304.2 (CmNaa35, encoding an N-α-acetyltransferase) was identified as the most likely candidate gene for ...

Melon wrinkles linked to protein N-terminal acetylation. link.springer.com/article/10.1...

Global profiling of N-terminal cysteine–dependent degradation mechanisms | PNAS Hypoxia, a condition characterized by insufficient oxygen supply, challenges cellular homeostasis and energy production, triggering adaptive respon...

Global profiling of N-terminal cysteine–dependent degradation mechanisms | PNAS | @ispt-proteinterm.bsky.social www.pnas.org/doi/10.1073/...

The evolutionary rise of oxygen sensing in land plants Land plants evolved from aquatic green algal ancestors to dominate terrestrial ecosystems approximately 480 million years ago during the Ordovician period (Hammarlund et al., 2020). This major event i...

Our 'Spotlight' discussing an excellent recent paper by @lauradc.bsky.social @n-end-rules.bsky.social & @syno2xis.bsky.social et al on the evolution of oxygen signalling in land plants is now online at Molecular Plant.

Spotlight: tinyurl.com/7ydn6p4a

Original paper: tinyurl.com/yw5yd4f8

📢 Our August issue is online!

🎨 The cover highlights a Feature Review from Stephanie Rosswag de Souza, @gzaffagnini.bsky.social, and @bokelab.bsky.social on how proteostasis functions in cellular dormancy across a number of examples, from yeast to adult quiescent stem cells and vertebrate oocytes.

Zebrafish model shows that NAA15 and N-terminal acetylation defects cause heart disease | Independently confirms the causal link between pathogenic NAA15 variants and congenital heart disease | Burns lab @bostonchildrens.bsky.social www.biorxiv.org/content/10.1...

Mechanism for voltage-gated channel inactivation uncovered | N-terminal acetylation of the intracellular domain of the Shaker potassium channel is key for plugging the pore | By Kenton Swartz lab in @nature.com @ispt-proteinterm.bsky.social www.nature.com/articles/s41...

How to define degrons and E3 ligase specificities by peptide pulldowns | Methods in Enzymology | Kwon and Ji labs | #KCMF1 #Cysteine #Arg/N-degron @ispt-proteinterm.bsky.social www.sciencedirect.com/science/arti...

Using a specific antibody to detect proteins carrying N-terminal formylation | Methods in Enzymology | Hwang lab @ispt-proteinterm.bsky.social www.sciencedirect.com/science/arti...

Assessing protein N-terminal myristoylation. Detailed protocols by Ed Tate's group in Methods in Enzymology. #lipidation @ispt-proteinterm.bsky.social @imperialcollegeldn.bsky.social www.sciencedirect.com/science/arti...

Manipulating plant oxygen sensing through NCO substitution reveals trade-offs between growth and flooding tolerance The oxygen-dependent degradation of Ethylene Response Factors VII (ERFVIIs) through the N-degron pathway is central to regulating the transcriptional responses to hypoxia in vascular plants. Plant Cys...

Can we modulate plant oxygen sensing to enhance flood resilience?🌿🔧

www.biorxiv.org/content/10.1...

This is one of two preprints out this week from the @syno2xis.bsky.social and @emilyflashman.bsky.social labs, two angles on how PCOs shape plant flood resilience. Don’t miss the full story!

Expression and purification of ribosome associated factors: Methionine aminopeptidases and N-terminal acetyltransferases | Methods in Enzymology | Marius Klein and Irmgard Sinning @uniheidelberg.bsky.social @ispt-proteinterm.bsky.social www.sciencedirect.com/science/arti...

How to express, purify and assess the activity of the ATE1 arginyltransferase | Methods in Enzymology | Yi Zhang lab @ispt-proteinterm.bsky.social www.sciencedirect.com/science/arti...

Method to determine the identity and modification state of plastid precursor protein N-termini in the cytosol by combining protoplast protein import assays with targeted mass spectrometry | Methods in Enzymology | Baginsky lab. @ispt-proteinterm.bsky.social www.sciencedirect.com/science/arti...

Bidirectional control of a metabolic transition by the GID ubiquitin ligase The GID/CTLH ubiquitin ligase is a multisubunit E3 conserved across eukaryotes. GID/CTLH has been implicated in a variety of processes, including metabolic regulation, cell proliferation, embryonic de...

Gid11 is a substrate receptor of the GID multisubunit E3 ligase during a metabolic switch. Specific degradation of proteins linked to glycolysis via their N-terminal threonine lacking N-terminal acetylation. #N-degron Khmelinskii lab @ispt-proteinterm.bsky.social
www.biorxiv.org/content/10.1...

Manipulating plant oxygen sensing through NCO substitution reveals trade-offs between growth and flooding tolerance The oxygen-dependent degradation of Ethylene Response Factors VII (ERFVIIs) through the N-degron pathway is central to regulating the transcriptional responses to hypoxia in vascular plants. Plant Cys...

Manipulating plant oxygen sensing through NCO substitution reveals trade-offs between growth and flooding tolerance. Perri et al. - another preprint from the Licausi and Flashman labs. @monicaperri1.bsky.social @emilyflashman.bsky.social @syno2xis.bsky.social www.biorxiv.org/content/10.1...

Engineering reduced activity in the oxygen-sensing Arabidopsis thaliana plant cysteine oxidase 4 enzyme results in improved flood resilience Plant cysteine oxidases (PCOs) are oxygen-sensing enzymes that play an important role in plant responses to low oxygen (hypoxia). PCO-catalysed dioxygenation of the N-terminal Cys of substrates, inclu...

Engineering reduced activity in the oxygen-sensing Arabidopsis thaliana plant cysteine oxidase 4 enzyme results in improved flood resilience. By Dirr et al. Licausi and Flashman labs @syno2xis.bsky.social @emilyflashman.bsky.social www.biorxiv.org/content/10.1...

Excited to announce our new methods paper in Methods in Enzymology!

We share our pipeline for building high-diversity DNA libraries & exploring protein stability in E. coli.

Free access until Sept 4: authors.elsevier.com/a/1lRkeHRzCX...
Updated, companion preprint: www.biorxiv.org/content/10.1...

New paper out in Mol Cell, where we show how N-myristolytransferases lipidate proteins on the translating ribosome!
Continuing our successful collaboration with DeuerlingLab @uni-konstanz.de & Shu-ou Chan @caltech.edu
@snsf.ch @ethz.ch
www.cell.com/molecular-ce...