Rajat Kumar Jha

Distribution of haploid chromosomes into separate nuclei in two pathogenic fungi Nuclei define eukaryotes, enabling macromolecular compartmentalization and cellular regulation. Each nucleus is believed to contain one or more haploid sets of chromosomes (1N). However, we discovered...

Distribution of haploid chromosomes into separate nuclei in two pathogenic fungi | Science www.science.org/doi/10.1126/...

Evolved gene editor inserts entire genes in human cells The new system is the first to use a DNA-mobilizing enzyme called a CRISPR-associated transposase to make targeted gene-sized edits at therapeutically useful levels in human cells.

Using laboratory-evolved versions of enzymes called CASTs, researchers have achieved a long-standing goal of genome editing: inserting entire healthy genes into human cells efficiently enough for potential therapeutic applications. @isaacwitte.bsky.social @eitzingersimon.bsky.social

Structure and mechanism of aminoacyl-tRNA-protein L/F- and R-transferases The aminoacyl-tRNA-protein transferases (also known as aa-transferases) are a class of enzymes that utilize a highly conserved GCN5-related N-acetyltr…

Here's one to add to your Friday reading list! 🚨Just in press in J. Mol. Biol.🚨: a beautiful review on the structures, mechanisms, regulation, and open questions of aminoacyl-tRNA-protein transferases by Misti and Rajat who work on amino acid transferases! Read the paper here:

tinyurl.com/mr386wsn

Measurements of molecular size and shape on a chip Size and shape are critical discriminators between molecular species and states. We describe a microchip-based high-throughput imaging approach offering rapid and precise determination of molecular pr...

An amazing new enabling technology. Measurements of molecular size and shape on a chip. Couldn't be prouder of our friend Madhavi Krishnan of @ox.ac.uk this was and is one among the must fun collaborations I ever had. www.science.org/doi/10.1126/...

The Cia1 and Cia2 subunits of the CTC mediate recognition of apo-FeS proteins with a C-terminal targeting complex recognition motif The cytosolic iron-sulfur cluster assembly (CIA) targeting complex is responsible for maturation of cytosolic and nuclear iron-sulfur enzymes, numbering >30 proteins critical for fundamental processes...

So excited to get this work out there! We have been working hard to "crack the CIA code" - more to come soon!
www.biorxiv.org/content/10.1...

In-cell architecture of the mitochondrial respiratory chain Mitochondria regenerate adenosine triphosphate (ATP) through oxidative phosphorylation. This process is carried out by five membrane-bound complexes collectively known as the respiratory chain, workin...

Wow. This is really stunning . www.science.org/doi/10.1126/...

NIH announces some key grant-review meetings will restart in late March Trump policy blocking required notices has frozen reviews of thousands of grants

At least some meetings essential to approving new grants by the National Institutes of Health will be back on track later this month. scim.ag/41FbfEg

AlphaFold has achieved significant success in protein prediction, even winning a Nobel Prize. AlphaFold3 now includes predictions for different molecules. What are the current limitations in predicting RNA structure? #3DRNAStructure #AlphaFold3 doi.org/10.1107/S205...