Reuven Gordon

The charge of a single protein gives an additional weak repulsion or attraction in a nanoaperture optical tweezer that can be used to measure the charge on single proteins.
iopscience.iop.org/article/10.1...

Accurate label free classification of cancerous extracellular vesicles using nanoaperture optical tweezers and deep learning - npj Biosensing npj Biosensing - Accurate label free classification of cancerous extracellular vesicles using nanoaperture optical tweezers and deep learning

Deep learning for accurate classification of extracellular vesicles from cancer and healthy cells. Delighted to have this successful New Frontiers Research Fund project published today: www.nature.com/articles/s44...

Inaugural Gordon Research Conference on Label-Free Single Molecule Sensing

Thank you for joining and sharing your label-free tech/science with us! Looking forward to: www.grc.org/single-molec...

Monitoring Conformational Dynamics of Single Unmodified Proteins using Plasmonic Nanotweezers Current single-molecule techniques to characterize proteins typically require labels, tethers, or the use of non-native solution conditions. Such changes ...

Label free dynamics of single proteins -- demo video. I love seeing the actual experiment demonstrating how straightforward this approach is (albeit Cuifeng and Mohsen's microfluidics is way more advanced than anything we have attempted).

app.jove.com/v/68093/moni...

Several Optica journals are seeking to fill Editor-in-Chief positions for 2026 for 3 years: opg.optica.org/info/awoc202...

Direct observation of conformational dynamics of intrinsically disordered proteins at the single-molecule level Intrinsically disordered proteins (IDPs) and proteins with intrinsically disordered regions (IDRs) are integral to many biological processes, including neurotransmitter regulation, microtubule regulat...

The energy landscape of a single disordered protein (tau) and the impact of PTMs is resolved. An important step to understanding the function and dysfunction of disordered proteins from their conformational ensemble. Not yet peer reviewed. www.biorxiv.org/content/10.1...

Great visit. Thanks to the labs of Johannes Stigler, Dieter Braun and also thanks to Ralf Jungmann, Philip Tinnefeld, Fritz Keilman for inspiring discussions. And managed to get a few runs in!

Looking forward to talking at LMU Center for Nanoscience www.cens.de/calendar/sum... tomorrow. Check it out in person or online!

Just had an editorially invited manuscript editorially rejected.

How do you watch the conformational dynamics of single molecules without using labels? Here is an excellent tutorial: bsky.app/profile/reuv... #singlemolecule #labelfree #biophysics

The modified announcements say that up to 15% of doctoral, and 20% of postdoctoral, awards can be held by international scholars.

Label-free Single Molecule Sensing -- the first conference in this Gordon Research Conference series. Outstanding distinguished invited speaker program just announced: www.grc.org/label-free-s...
Submit your applications early!
#singlemolecule #labelfree

Conformational Stability at Low Temperatures Using Single Protein Nanoaperture Optical Tweezers Nanoaperture optical tweezers allow for trapping single proteins and detecting their conformational changes without modifying the protein, i.e., being free from labels or tethers. While past works have used laser heating as a way to vary the local temperature, this does not allow for probing of lower temperature values. Here we investigate the lower temperature dynamics of individual Bovine Serum Albumin (BSA) proteins with the help of a custom Peltier cooling stage. The BSA transitions between the normal (N) and fast (F) states. The normal form of BSA has a maximum occupancy at 21 ± 1 °C, which is interpreted as its maximum stability point for the compact N form with respect to the F form. In this way, it is possible to find the relative thermodynamic parameters of single proteins without requiring any modifications to the intrinsic structure.

Stability maximum of protein conformations observed on a single molecule using real-time label-free approach. #biophysics #singlemolecule
#NOTs
pubs.acs.org/doi/10.1021/...

After lunch, we kicked off the afternoon session with Reuven Gordon from the University of Victoria. He delivered an enlightening presentation on 'Nanoaperture Optical Tweezers for Single Unmodified Protein Dynamics
#SMPS4

Direct observation of small molecule activator binding to single PR65 protein npj Biosensing - Direct observation of small molecule activator binding to single PR65 protein

rdcu.be/d6qG4 Single molecule label-free small molecule interactions for drug discovery. SMAPs. Outstanding work by Annie, Maria and co-authors. #singlemolecule #labelfree #drugdiscovery

Direct observation of small molecule activator binding to single PR65 protein npj Biosensing - Direct observation of small molecule activator binding to single PR65 protein

rdcu.be/d6qG4 Single molecule label-free small molecule interactions for drug discovery. SMAPs. Outstanding work by Annie, Maria and co-authors. #singlemolecule #labelfree #drugdiscovery

Great visit Down Under. NFO17 and Qubic.

Another great NFO - Brno in 2026. Thanks @sile-nicchormaic.bsky.social for pic!