The Freeman Lab

HeLa cells stably expressing a dox-inducible active TMEM16F-GFP (blue). Once expressed, lipid scrambling of the plasma membrane bilayer causes vesiculation, shown with Annexin V staining of the exposed PS (gold).

We’ve engineered stable cell systems to induce lipid scrambling of the plasma membrane with active forms of TMEM16F. Scrambling alone causes vesiculation (🔵TMEM16F-active; 🟠PS/Annexin V). Let us know if you want to try the cells out. Preprint: www.biorxiv.org/content/10.1...

14.05.2025 22:46 — 👍 23    🔁 4    💬 0    📌 0

Sorting nexin 10 regulates lysosomal ionic homeostasis via ClC-7 by controlling PI(3,5)P2, say Jing Ze Wu, Sergio Grinstein, Spencer Freeman and colleagues @sickkidsto.bsky.social: rupress.org/jcb/article/...

#Immunology #Membrane #lipid #lysosomes #osteopetrosis

02.04.2025 18:17 — 👍 7    🔁 3    💬 1    📌 0

Sorting nexin 10 regulates lysosomal ionic homeostasis via ClC-7 by controlling PI(3,5)P2 Snx10 was found to determine the level of PI(3,5)P2 in (phago)lysosomes by controlling the supply of PI(3)P from upstream endocytic compartments. By regula

This work was done in tandem with cool work from the @spencerfreeman.bsky.social lab identifying novel regulators of PI3,5P2 in lysosomes
rupress.org/jcb/article-...

31.03.2025 16:25 — 👍 7    🔁 2    💬 0    📌 0

Pemberton, Burke, Balla et al. report the generation & characterization of an engineered active fragment of PIKfyve and its use to generate extra PI(3,5)P2 in endomembrane compartments & test the function of PI(3,5)P2 #biosensors rupress.org/jcb/article/...

#lipids #CellSignaling

26.03.2025 19:17 — 👍 17    🔁 3    💬 1    📌 0

New tools and regulators of lysosomal PI(3,5)P2!
Lipidomics by Sin, Junko, Takehiko (Tokyo) and a new toolkit for PIKfyve/PI(3,5)P2 by Josh and Tamas (NIH). Check out JIm's paper here: hubs.la/Q03dym0b0 or: shorturl.at/yq9K8 and the paper by Balla, Burke, Pemberton et al. here: hubs.la/Q03dyfL60

27.03.2025 17:27 — 👍 4    🔁 0    💬 0    📌 0

Matrix-producing neutrophils populate and shield the skin Nature - A population of neutrophils in the skin produces extracellular matrix, providing a defence strategy by reinforcing the barrier properties of the skin and helping to block the entry of...

Excited to post for the first time to present our exciting new paper @Nature. The fruit of massive work by Tommaso Vicanolo, @alazozcan.bsky.social and Jackson Li, and our superb collaborators Laiguan and Stefan Uderhardt. True teamwork!
Free link: rdcu.be/eeeA2
www.nature.com/articles/s41...

19.03.2025 21:28 — 👍 66    🔁 27    💬 8    📌 5

Simultaneous CRISPR screening and spatial transcriptomics reveal intracellular, intercellular, and functional transcriptional circuits Perturb-FISH, a method that combines high-throughput imaging spatial transcriptomics with in situ guide RNA detection, allows for the analysis of molecular and functional states, with spatial resoluti...

Simultaneous CRISPR screening and spatial transcriptomics reveal intracellular, intercellular, and functional transcriptional circuits: Cell www.cell.com/cell/fulltex...

12.03.2025 22:04 — 👍 2    🔁 0    💬 0    📌 0

We've been studying mechanisms of resilience that protect lysosomes from damage and wrote a review on the topic. We consider lysosomal stress and damage on a spectrum. Please help me share as this is my first post 😀 and I don't have many followers! 🙏 Open Access: www.embopress.org/doi/full/10....

27.02.2025 15:54 — 👍 61    🔁 43    💬 0    📌 4