Astha Neupane

It was nice meeting you again at grc. Your talk was amazing and gave me a lot to think about.

05.06.2025 05:42 — 👍 1    🔁 0    💬 0    📌 0

Sorting nexin 10 regulates lysosomal ionic homeostasis via ClC-7 by controlling PI(3,5)P2, say Jing Ze Wu, Sergio Grinstein, Spencer Freeman and colleagues @sickkidsto.bsky.social: rupress.org/jcb/article/...

#Immunology #Membrane #lipid #lysosomes #osteopetrosis

02.04.2025 18:17 — 👍 7    🔁 3    💬 1    📌 0

HeLa cells stably expressing a dox-inducible active TMEM16F-GFP (blue). Once expressed, lipid scrambling of the plasma membrane bilayer causes vesiculation, shown with Annexin V staining of the exposed PS (gold).

We’ve engineered stable cell systems to induce lipid scrambling of the plasma membrane with active forms of TMEM16F. Scrambling alone causes vesiculation (🔵TMEM16F-active; 🟠PS/Annexin V). Let us know if you want to try the cells out. Preprint: www.biorxiv.org/content/10.1...

14.05.2025 22:46 — 👍 23    🔁 4    💬 0    📌 0

We've been studying mechanisms of resilience that protect lysosomes from damage and wrote a review on the topic. We consider lysosomal stress and damage on a spectrum. Please help me share as this is my first post 😀 and I don't have many followers! 🙏 Open Access: www.embopress.org/doi/full/10....

27.02.2025 15:54 — 👍 61    🔁 43    💬 0    📌 4

In my first real post I want to show the key experiment that revealed to me what I want my own lab to be about:
The conversion of phosphoinositides!
By using fluorescent biosensors and membrane coated beads I was able to reconstitute the real time kinetics of PI(4)P to PI(4,5)P2 conversion by PIP5K

18.11.2024 16:37 — 👍 70    🔁 14    💬 2    📌 0