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shaio

@shaio.bsky.social

ghostboy / plant molecular biology / diy / three racoons in a lab coat

92 Followers  |  142 Following  |  125 Posts  |  Joined: 07.09.2023  |  2.1939

Latest posts by shaio.bsky.social on Bluesky

I know it's nasty stuff, but TRIzol (and clones) based protocols work pretty well, and are much cheaper.

23.10.2025 05:23 — 👍 2    🔁 0    💬 0    📌 0

Организацията на Тракия е безумна. Има някакви часове, в които уж спират движението в посока София, но те не се спазват (както и продължителността).

Ходенето на събития в София е почти невъзможно в момента.

16.10.2025 15:50 — 👍 0    🔁 0    💬 0    📌 0

Oh, good. The Torment Nexus that already has people falling in love with it, and making them lose their minds, will now also get them horny. What could go wrong.

15.10.2025 04:26 — 👍 0    🔁 0    💬 0    📌 0
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Fantastical flowers, synthetic biology, and storytelling.

Giving an accessible science talk tomorrow at 10:40 UK time... come join!

Register here for the link 👉 jic.link/NBIAST2025

06.10.2025 12:38 — 👍 40    🔁 18    💬 1    📌 1

Very excited to see our @nikogeldner.bsky.social lab x Feng Zhou lab work featured on the cover of Science!
(1/5) We reveal how root architecture and nutrient leakage shape spatial patterns of microbial colonization, moving beyond traditional models of uniform exudation.

02.10.2025 22:35 — 👍 103    🔁 42    💬 8    📌 1

Thank you!

I'll do a gradient of IAA and see how it goes.

01.10.2025 06:31 — 👍 1    🔁 0    💬 0    📌 0

I was also thinking - maybe cutting down the sucrose (now at 20-30 g/L), could have effect?

30.09.2025 04:52 — 👍 0    🔁 0    💬 0    📌 0

It's full strength MS, Nitsch vitamins and:
- started with no hormones (per source);
- after a month of no results moved to 0.5 mg/L BAP + 0.1 mg/L IAA;
- now I'm at 0.1 mg/L IAA (no BAP) and still nothing.

I move them to fresh media every two weeks.

I'll try to source clonex rooting gel.

30.09.2025 04:50 — 👍 1    🔁 0    💬 1    📌 0

Hey Seb, random question, but am in a pinch - my tomato shoots are not rooting. Any hints? I've changed media (different hormones) three times already and still zilch.

30.09.2025 04:06 — 👍 2    🔁 0    💬 1    📌 0
Preview
Blooms supersized - Nature Biotechnology Nature Biotechnology - Blooms supersized

A tiny white-petalled weed beloved of plant biologists has received a CRISPR-fueled makeover, turning it into a supersized ruby-colored bloom www.nature.com/articles/s41...

26.09.2025 16:01 — 👍 36    🔁 18    💬 0    📌 1
Post image

Meet "Little Flower".

A genetically engineered flower, frozen in time and blown up over 150X. Here's the story (1/6 🧵)

23.09.2025 16:00 — 👍 29    🔁 6    💬 1    📌 0

като не мога да намеря някоя стара песен в нета, ми се струва, че трябва да съм я сънувала. някой сеща ли се за един канон, пееше се "есен, тиха есен, чувам твойта тъжна песен, чувам аз твойта тъжна песен"? имаше и "грей в ползата" за гората

14.09.2025 15:26 — 👍 0    🔁 2    💬 1    📌 0

They even consume it, the freeloaders they are...

08.09.2025 12:46 — 👍 2    🔁 0    💬 0    📌 0

Присъединявам се към ползването на ръмбав от десетилетия. Особено за неща, върху които седиш. Също "ръмби ми", примерно, когато се оплакваш от стол/диван

08.09.2025 11:56 — 👍 3    🔁 0    💬 0    📌 0

For context that's a third the price the RIAA expected a single human to pay for just downloading 24 songs a decade ago (after cutting the ruling down from $1.5m, or $62,000 per song), and that's not adjusting for inflation:

05.09.2025 19:33 — 👍 2328    🔁 1158    💬 5    📌 19

Абсолютен регрес. Вместо да започне по-силно налагане на "продължението на тротоара е пешеходна пътека", което масово не се спазва от шофьорите (и не се знае от пешеходците), нещата стават "пешеходната пътека е продължение на платното"...

05.09.2025 04:32 — 👍 15    🔁 2    💬 0    📌 0

Крайно време е да забранят ходенето пеша. Всеки в кола, за да има ред

05.09.2025 04:28 — 👍 3    🔁 0    💬 0    📌 0

bsky.app/profile/shai...

05.09.2025 04:27 — 👍 0    🔁 0    💬 0    📌 0

Having done this a few days ago - sigh.

03.09.2025 15:40 — 👍 0    🔁 0    💬 0    📌 0

Забърках едно ГМО. Утре ще пробвам с друго :>

27.08.2025 17:04 — 👍 2    🔁 0    💬 0    📌 0

Да, така си е. Ако не познаваш как и къде да се престроиш, попадаш в много тъпи ситуации. Тук доста рядко пускат да се мушнеш.

Градът наистина е приятен, но придвижването в него е леко тралала.

27.08.2025 17:02 — 👍 1    🔁 0    💬 0    📌 0

Точно така се чувствам и аз. Да паля колата само, когато трябва да пренеса нещо или за някакви по-завъртяни пътешествия.

27.08.2025 16:48 — 👍 1    🔁 0    💬 0    📌 0

На зелен пешеходен светофар, с коли които правят ляв завой, ми спря буквално последната кола. Въпреки, че им махах възмутено. Въпреки, че бутах колело и бях с шарен велосипеден екип (доста съм видим). Туй то. Див Пловдив.

27.08.2025 16:41 — 👍 0    🔁 0    💬 1    📌 1

Докато живеех в София - велосипед и градски. Междуградските разходки - с влак и автобус, понякога на стоп.

26.08.2025 08:48 — 👍 1    🔁 0    💬 1    📌 0

This phenotype is also observed after heat stress events. Normal when you're doing an experiment, quite annoying when that's the way you discover that cooling in the chamber had failed.

26.08.2025 04:23 — 👍 4    🔁 0    💬 1    📌 0

Не е търсеният ефект, но за мен имаше полза - заради скапания градски най-накрая изкарах книжка. Ако не беше Пловдив, още щях да се дърпам.

26.08.2025 04:18 — 👍 3    🔁 0    💬 2    📌 0

Мичурин е бил доста добър учен и е допринесъл доста за селекцията и генетиката. За разлика от него Лисенко е бил ужасен човек, ужасно смотан като "учен" и приносът му общо взето е прецакването на Вавилов (важен ботаник и генетик), както и смъртта на милиони, след като идеите му се прегръщат от Мао.

23.08.2025 20:52 — 👍 2    🔁 0    💬 1    📌 0
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Something very strange is going on
There is a seemingly coordinated effort to FLIP FLOP on Al. All of the execs who were hyping Al to death just a few weeks ago are now claiming that Al is a bubble, that AGI is nowhere near, and that Al will have a much smaller impact than anticipated.
> Altman called it a bubble
> Eric Schmidt said that AGI is basically bullshit
> Matt Garman (AWS CEO) downplayed its impact
WHY the sudden narrative switch?
WHO gave the orders?
I swear, it's like they're all part of some cult. They all embrace the same talking points all at once, and abandon them all at once.

Community Salaries Reviews Jobs For Business v Tech Industry • 1h Amazon • EL TRUMP Something very strange is going on There is a seemingly coordinated effort to FLIP FLOP on Al. All of the execs who were hyping Al to death just a few weeks ago are now claiming that Al is a bubble, that AGI is nowhere near, and that Al will have a much smaller impact than anticipated. > Altman called it a bubble > Eric Schmidt said that AGI is basically bullshit > Matt Garman (AWS CEO) downplayed its impact WHY the sudden narrative switch? WHO gave the orders? I swear, it's like they're all part of some cult. They all embrace the same talking points all at once, and abandon them all at once.

Oh yeah things are falling apart. You've got paranoid guys on Blind freaking out now

21.08.2025 05:14 — 👍 4252    🔁 659    💬 147    📌 163
An old image of a salivary gland chromosome from Drosophila melanogaster, with lines across different segments indicating contact points.

An old image of a salivary gland chromosome from Drosophila melanogaster, with lines across different segments indicating contact points.

The original circos plot? From the 1947-1948 Carnegie Yearbook, the page prior to McClintock's Mutable Loci in Maize paper.

16.08.2025 16:24 — 👍 88    🔁 35    💬 4    📌 0

Ah, yes, my plain text emails from 10 years ago are the problem, not the "AI" bullshit that wants its own nuclear powerplants. Of course.

12.08.2025 20:23 — 👍 4    🔁 1    💬 0    📌 0

@shaio is following 20 prominent accounts