Sylvie Callegari

Really enjoyed the #ASBMB2025 conference in Brisbane this week. It's an honour to get to present to this audience and winning an oral presentation prize was an unexpected bonus!

ProteinDJ logo

I am thrilled to release ProteinDJ: a high-performance and modular protein design pipeline. Our open-source workflow incorporates #RFdiffusion, #ProteinMPNN, #FAMPNN, #AlphaFold2 and #Boltz-2. It is a fast, free, and fun way to design proteins (1/5)
doi.org/10.1101/2025.09.24.678028 #proteindesign

📸 L–R: Professor David Komander, Dr Nicholas Kirk, Dr Sylvie Callegari and Dr Alisa Glukhova. 

Researchers from WEHI’s Parkinson Disease Research Centre have won the 2025 UNSW Eureka Prize for Scientific Research. The team has been honoured for their research unravelling how PINK1, a key protein linked to early-onset Parkinson’s disease, works.

🔗 www.wehi.edu.au/news/eureka-...

Very exciting to see the @wehi-research.bsky.social PINK1 Parkinson’s Disease research team win the Eureka Prize for Scientific Research. Amazing achievement by a brilliant team! @eurekaprizes.bsky.social

The Institute for Molecular Bioscience is seeking an exceptional academic leader to be the new Director of the Centre for Chemistry and Drug Discovery and lead our centre consisting of 10 group leaders, 40 staff and approximately 50 HDR students. Come and join us in sunny Brisbane!

Glad to share the final version of our story about the UBR4 complex, an E4 ligase protein quality control hub @science.org. Now with more cryo-EM structures and a deeper dive into substrate recognition, especially escaped mitochondrial proteins @clausenlab.bsky.social www.science.org/doi/10.1126/...

Our June Newsletter highlights IUBMB activities including a note from the President and updates on the IUBMB Trainee Initiative

➡️http://bit.ly/45ai6r1

PINK1 Parkinson's Disease Research Team @wehi-research.bsky.social is a finalist in the @unsw.edu.au Eureka Prize for Scientific Research.

Learn more: youtu.be/senXARXzO20

#EurekaPrizes @komanderlab.bsky.social @zhongyangan.bsky.social @sylviecallegari.bsky.social @nskirk.bsky.social

YouTube video by Australian Museum PINK1 Parkinson's Disease Research Team – Finalist, 2025 Eureka Prize for Scientific Research

🎉 What an honour to be a finalist in the 2025 Australian Museum Eureka Prizes for Scientific Discovery!! With David Komander, @zhongyangan.bsky.social, Alisa Glukhova and @nskirk.bsky.social.

Excited for the awards ceremony on Sept 3rd!

@eurekaprizes.bsky.social
@wehi-research.bsky.social

It All Comes Down to the Mitochondria

“Why do we sleep” has been a surprisingly hard question. But it looks like the answer may be “ Because we breathe”. Our mitochondria need a break!

📣 Calling all ubiquitin aficionados - one week to go before abstract deadline (July 18th)!! Join us on the coast in Lorne, Australia. www.lorneubiquitin.org
#ubiquitin

Australia’s First Ubiquitin Summit is here! 🥳
📍Lorne, Victoria | 🗓️ 20–23 November 2025

Join us on the scenic coastline of Lorne for 4 days of cutting-edge ubiquitin science, featuring a fantastic lineup of international speakers covering all corners of the Ub world—from mechanisms to therapeutics

Silencing mitochondrial gene expression in living cells Mitochondria fulfill central functions in metabolism and energy supply. They express their own genome, which encodes key subunits of the oxidative phosphorylation system. However, central mechanisms u...

Researchers in Science have developed a tool to silence specific mitochondria-encoded mRNAs in living cells, which could help scientists understand the origins of certain mitochondria-related diseases. scim.ag/3F6Wm5d

Excited that our work revealing the structure and function of the monster stress response silencing factor, the E3 ligase SIFI, is now out! Congratulations to the most amazing team of Zhi Yang, Diane Haakonsen, Michael Heider and Sam Witus!

www.nature.com/articles/s41...

Transport of cytoplasmically synthesized proteins into the mitochondria in a cell free system from Neurospora crassa - PubMed Synthesis and transport of mitochondrial proteins were followed in a cell-free homogenate of Neurospora crassa in which mitochondrial translation was inhibited. Proteins synthesized on cytoplasmic rib...

I looked this up for a review I wrote a few years ago. The PNAS paper is definitely one of the first, but there was an earlier version of the in vitro import assay published by the Neupert lab in 1977 pubmed.ncbi.nlm.nih.gov/145943/.

Stunning photo of Mars on the cover of Science this week! And super proud that our structure of PINK1 stalled at mitochondrial translocases also gets a cover mention 😊. It's now out in print on page 303!

A novel PLpro inhibitor improves outcomes in a pre-clinical model of long COVID - Nature Communications A novel antiviral targeting the SARS-CoV-2 PLpro protease shows strong efficacy in a mouse model, preventing lung pathology and reducing brain dysfunction. The study provides proof-of-principle that P...

Excited to share news from the lab out in Nature Communications – A novel PLpro inhibitor improves outcomes in a pre-clinical model of long COVID
www.nature.com/articles/s41...
@wehi-research.bsky.social

Join us at the beach for the first Australian Ubiquitin summit from 20-23 Nov! 🏄‍♀️ Check out the amazing speakers here: www.lorneubiquitin.org

And, why not stay a little longer and join the International Symposium on Autophagy from 16-20 Nov, also in beautiful Lorne ☀️. www.isa11.org

See you there!

Human PINK1 at a TOM-VDADC array
Structure of the Week/Month/Year(?)! 9eih.
Sylvia Callegari...David Komander, Science 13 March 2025.
#coot Fancy mode
Copyright/license-free - i.e. freely reproducible.
Coot 1.1.14 coming soon!

PINK1/Parkin-dependent mitophagy caught in situ by cryo-ET! New preprint www.biorxiv.org/content/10.1... led by @kev-in-situ-et.bsky.social. This has been a long-standing goal of team @mito911.bsky.social within the @asapresearch.parkinsonsroadmap.org initiative.

Thanks Malte!

I'm super happy that our story is now published!
📖 www.science.org/doi/10.1126/...
But what changed compared to the original preprint?

Also, I feel i should post Movie 1 🎥, that inspired the cover. Back when I did the original bluesky thread, movies were not available.

We're on the cover of @science.org this week. Awesome work by @florentwaltz.bsky.social. #CryoET is reaching crazy resolutions inside cells, and this is just the beginning. Fantastic interpretation of the proton motive force by @verenaresch.bsky.social. Always a pleasure making #SciArt with you!🧪🧶🧬🌾

Thanks Brett 😊

Thank you Sharad!

Thanks Di 😊

It really is!

😂

Thanks James! 😂

Thrilled to share the structure of dimerised human PINK1 docked to an endogenous translocase array on the mitochondrial surface, composed of two TOM complexes, bridged by a VDAC2 dimer! Published today in Science www.science.org/doi/10.1126/...

@wehi-research.bsky.social @komanderlab.bsky.social