The Interactive Fly

Huang, Y. C., Costa, C. A. M., Ruiz, N. V., Wang, X., Jevitt, A., Breneman, C. M., Han, C., Deng, W. M. (2025). Polyploidy promotes transformation of epithelial cells into non-professional phagocytes. bioRxiv, www.biorxiv.org/content/10.1...

Singh, J., Verma, D., Sarkar, B., Paul, M., Mutsuddi, M., Mukherjee, A. (2025). Notch and LIM-homeodomain protein Arrowhead regulate each other in a feedback mechanism to play a role in wing and neuronal development in Drosophila. Open Biol, 15(4):240247 royalsocietypublishing.org/rsob/article...

Functional characterization of eicosanoid signaling in Drosophila development Author summary There are numerous bioactive lipids that control animal physiology, and some of them are commonly observed in both humans and insects. Well-studied insects such as fruit flies can there...

Fujinaga, D., Nolan, C., Yamanaka, N. (2025). Functional characterization of eicosanoid signaling in Drosophila development. PLoS Genet, 21(5):e1011705 journals.plos.org/plosgenetics...

HIF-1α-mediated feedback prevents TOR signalling from depleting oxygen supply and triggering stress during normal development - Nature Communications During normal development, growing fly wing precursors become hypoxic, triggering HIF-1α-mediated negative feedback on TOR signalling and growth deceleration. Without this feedback, the mismatch betwe...

Zhao, Y., Alexandre, C., Kelly, G., Vincent, J. P., Perez-Mockus, G. (2025). HIF-1alpha-mediated feedback prevents TOR signalling from depleting oxygen supply and triggering stress during normal development. Nat Commun, www.nature.com/articles/s41...

Neuroendocrine control of intestinal regeneration through the vascular niche in Drosophila Adult intestinal plasticity and tissue repair involves complex and reciprocal multicellular interactions. Medina, Perochon, et al. report a signaling axis between hormone-producing cells in the intest...

Medina, A. B., Perochon, J., Tian, Y., Johnson, C. T., Holcombe, J., Ramesh, P., Polcownuk, S., Yu, Y., Cordero, J. B. (2025). Neuroendocrine control of intestinal regeneration through the vascular niche in Drosophila. Dev Cell, 60(22):3085-3101 www.cell.com/developmenta...

Protein-responsive gut hormone tachykinin directs food choice and impacts lifespan - Nature Metabolism The gut hormone tachykinin (Tk) is found to be regulated by protein intake in flies and mice. In flies, Tk is shown to impact food choices, sleep and lifespan.

Ahrentlov, N., Kubrak, O., Lassen, M., ...., Nagy, S., Imig, C., Richter, E. A., Texada, M. J., Rewitz, K. (2025). Protein-responsive gut hormone tachykinin directs food choice and impacts lifespan. Nat Metab, 7(6):1223-1245 www.nature.com/articles/s42...

Arnce, L. R., Bubnell, J. E., Aquadro, C. F. (2025). Comparative Analysis of Drosophila Bam and Bgcn Sequences and Predicted Protein Structural Evolution. J Mol Evol, 93(2):278-291 pubmed.ncbi.nlm.nih.gov/40178596/

Isolating selective from non-selective forces using site frequency ratios Author summary A new statistical method is presented for estimating the distribution of strengths of natural selection acting on mutations in natural populations using the distribution of polymorphic ...

Hey, J., Pavinato, V. A. C. (2025). Isolating selective from non-selective forces using site frequency ratios. PLoS Genet, 21(4):e1011427 journals.plos.org/plosgenetics...

Comparative Analysis of Drosophila Bam and Bgcn Sequences and Predicted Protein Structural Evolution - Journal of Molecular Evolution The protein encoded by the Drosophila melanogaster gene bag of marbles (bam) plays an essential role in early gametogenesis by complexing with the gene product of benign gonial cell neoplasm (bgcn) to...

Arnce, L. R., Bubnell, J. E., Aquadro, C. F. (2025). Comparative Analysis of Drosophila Bam and Bgcn Sequences and Predicted Protein Structural Evolution. J Mol Evol, 93(2):278-291 link.springer.com/article/10.1...

Lai, W. Y., Hsu, S. K., Futschik, A., Schlotterer, C. (2025). Pleiotropy increases parallel selection signatures during adaptation from standing genetic variation. Elife, 13 elifesciences.org/articles/102...

Expression of De Novo Open Reading Frames in Natural Populations of Drosophila melanogaster We show that newly-evolved, expressed open reading frames (neORFs) identified in a set of inbred Drosophila melanogaster lines are also expressed in multiple tissues and developmental stages of poole...

Glaser-Schmitt, A., Lebherz, M., Saydam, E., Bornberg-Bauer, E., Parsch, J. (2025). Expression of De Novo Open Reading Frames in Natural Populations of Drosophila melanogaster onlinelibrary.wiley.com/doi/10.1002/...

Isolating selective from non-selective forces using site frequency ratios Author summary A new statistical method is presented for estimating the distribution of strengths of natural selection acting on mutations in natural populations using the distribution of polymorphic ...

Hey, J., Pavinato, V. A. C. (2025). Isolating selective from non-selective forces using site frequency ratios. PLoS Genet, 21(4):e1011427 journals.plos.org/plosgenetics...

The role of resource defensibility in facilitating sexually selected weapon evolution: an experimental evolution test Abstract. Animal weapons have evolved multiple times, primarily for battling for access to mates. Despite intra-sexual selection being common, exaggerated

Audet, T., Wilson, A., Dukas, R., Dworkin, I. (2025). he role of resource defensibility in facilitating sexually-selected weapon evolution: An experimental evolution test. Evolution, academic.oup.com/evolut/artic...

Comparative Analysis of Drosophila Bam and Bgcn Sequences and Predicted Protein Structural Evolution - Journal of Molecular Evolution The protein encoded by the Drosophila melanogaster gene bag of marbles (bam) plays an essential role in early gametogenesis by complexing with the gene product of benign gonial cell neoplasm (bgcn) to...

Arnce, L. R., Bubnell, J. E., Aquadro, C. F. (2025). omparative Analysis of Drosophila Bam and Bgcn Sequences and Predicted Protein Structural Evolution. J Mol Evol, 93(2):278-291 link.springer.com/article/10.1...

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Huang, Y. C., Costa, C. A. M., Ruiz, N. V., Wang, X., Jevitt, A., Breneman, C. M., Han, C., Deng, W. M. (2025). Polyploidy promotes transformation of epithelial cells into non-professional phagocytes. bioRxiv, www.biorxiv.org/content/10.1...

Singh, J., Verma, D., Sarkar, B., Paul, M., Mutsuddi, M., Mukherjee, A. (2025). Notch and LIM-homeodomain protein Arrowhead regulate each other in a feedback mechanism to play a role in wing and neuronal development in Drosophila. Open Biol, 15(4):240247 royalsocietypublishing.org/rsob/article...

Functional characterization of eicosanoid signaling in Drosophila development Author summary There are numerous bioactive lipids that control animal physiology, and some of them are commonly observed in both humans and insects. Well-studied insects such as fruit flies can there...

Fujinaga, D., Nolan, C., Yamanaka, N. (2025). Functional characterization of eicosanoid signaling in Drosophila development. PLoS Genet, 21(5):e1011705 journals.plos.org/plosgenetics...

Neuroendocrine control of intestinal regeneration through the vascular niche in Drosophila Adult intestinal plasticity and tissue repair involves complex and reciprocal multicellular interactions. Medina, Perochon, et al. report a signaling axis between hormone-producing cells in the intest...

Medina, A. B., Perochon, J., Tian, Y., Johnson, C. T., Holcombe, J., Ramesh, P., Polcownuk, S., Yu, Y., Cordero, J. B. (2025). Neuroendocrine control of intestinal regeneration through the vascular niche in Drosophila. Dev Cell, 60(22):3085-3101 www.cell.com/developmenta...

Protein-responsive gut hormone tachykinin directs food choice and impacts lifespan - Nature Metabolism The gut hormone tachykinin (Tk) is found to be regulated by protein intake in flies and mice. In flies, Tk is shown to impact food choices, sleep and lifespan.

Ahrentlov, N., Kubrak, O., Lassen, M., Malita, A., Koyama, T., ...., Nagy, S., Imig, C., Richter, E. A., Texada, M. J., Rewitz, K. (2025). Protein-responsive gut hormone tachykinin directs food choice and impacts lifespan. Nat Metab, 7(6):1223-1245 www.nature.com/articles/s42...

Evdokimova, A. A., Kolesnikova, T. D., Mazina, M. Y., ...., Vorobyeva, N. E. (2025). Transcriptional induction by ecdysone in Drosophila salivary glands involves an increase in chromatin accessibility and acetylation. Nucleic Acids Res, 53(7) pmc.ncbi.nlm.nih.gov/articles/PMC...

Evdokimova, A. A., Kolesnikova, T. D., Mazina, M. Y., ...., Vorobyeva, N. E. (2025). Transcriptional induction by ecdysone in Drosophila salivary glands involves an increase in chromatin accessibility and acetylation. Nucleic Acids Res, 53(7) pmc.ncbi.nlm.nih.gov/articles/PMC...

Drosophila melanogaster as a model organism to investigate sex specific differences - Scientific Reports Scientific Reports - Drosophila melanogaster as a model organism to investigate sex specific differences

Dahn, S., Wagner, A. E. (2025). Drosophila melanogaster as a model organism to investigate sex specific differences. Sci Rep, 15(1):19648 www.nature.com/articles/s41...

Upstream open reading frames dynamically modulate CLOCK protein translation to regulate circadian rhythms and sleep Oscillations of core clock genes, including Clock (Clk), are essential for the generation of circadian rhythms. This study reveals that upstream open reading frames are enriched in Drosophila circadia...

Sun, Y., Shui, K., Li, Q., Liu, C., Jin, W., Ni, J. Q., Lu, J., Zhang, L. (2025). Upstream open reading frames dynamically modulate CLOCK protein translation to regulate circadian rhythms and sleep. PLoS Biol, 23(5):e3003173 journals.plos.org/plosbiology/...

Fallacaro, S., Mukherjee, A., Turner, M. A., Garcia, H. G., Mir, M. (2025). Transcription factor hubs exhibit gene-specific properties that tune expression. bioRxiv, www.biorxiv.org/content/10.1...

Kemp, J. P., Jr., Geisler, M. S., Hoover, M., Cho, C. Y., O'Farrell, P. H., Marzluff, W. F., Duronio, R. J. (2025). Cell-cycle-regulated transcriptional pausing of Drosophila replication-dependent histone genes. Mol Biol Cell, 36(7):ar88 www.molbiolcell.org/doi/10.1091/...

Mouse promoters are characterised by low occupancy and high turnover of RNA polymerase II - Molecular Systems Biology The general transcription machinery and its occupancy at promoters are highly conserved across metazoans. This contrasts with the kinetics of mRNA production that considerably differ between model spe...

Chatsirisupachai, K., Moene, C. J. I., Kleinendorst, R., Kreibich, E., Molina, N., Krebs, A. (2025). Mouse promoters are characterised by low occupancy and high turnover of RNA polymerase II. Mol Syst Biol, 21(5):447-471 link.springer.com/article/10.1...

Undead cells up-regulate cytosolic Ca2+ signaling and produce Ca2+ flashes in a Dronc-dependent manner. Top: The disc outline is marked by yellow dashed lines. The white dotted lines highlight the ey-Gal4-expressing area of the eye discs. For all panels, scale bars are 50 μm. Confocal images of third instar larval eye imaginal disc expressing the Ca2+ reporter GCaMP6s in control (ey-Gal4, left, and ey>p35, middle) and in overgrown, undead (ey>hid,p35, right) discs. The GCaMP6s fluorescence directly represents the cytosolic Ca2+ level. The cytosolic Ca2+ level is significantly upregulated in undead (ey>hid,p35) discs compared to control discs (see white arrows in righthand panel). Bottom: Representative Ca2+ traces of eye imaginal discs expressing GCaMP6s obtained by time-lapse confocal imaging (600 frames, 1-second intervals). Ca²⁺ flashes refer to transient, localized increases in intracellular calcium levels, detected as brief bursts of elevated GCaMP6s fluorescence. These flashes are characterized by a rapid onset, short duration, and return to baseline, indicating discrete calcium signaling events within imaginal disc cells. Each line represents an independent disc (numbered). Undead discs display a strong increase in the number of Ca2+ flashes. Genotypes as for top row.

The initiator #caspase Dronc can trigger compensatory #apoptosis -induced proliferation (AiP). This study shows that Dronc-dependent Ca2+ influx into the cytosol is an important contributor to AiP & DUOX activation in #Drosophila (& likely in vertebrates) @plosbiology.org 🧪 plos.io/4pNG4PK

Efficient RFLP-based Protocol for Routine Authentication of Drosophila #micropublication #biology #data #caltechlibrary #Drosophila #GenotypeData #Methods #Methodology https://micropublication.org/journals/biology/micropub-biology-001949

Genome-wide analysis reveals genes mediating resistance to paraquat neurodegeneration in Drosophila Paraquat is linked to increased PD risk and to dopaminergic neurodegeneration in animal studies. Gene-environment interactions likely influence whether an

Villalobos-Cantor, S., Arreola-Bustos, A., Martin, I. (2025). Genome-wide analysis reveals genes mediating resistance to paraquat neurodegeneration in Drosophila. Genetics, 230(3) academic.oup.com/genetics/art...