The Interactive Fly

Phylogenetic and functional diversity among Drosophila-associated metagenome-assembled genomes | mSystems While much attention has been given to catalogue the taxonomic diversity intrinsic to host-associated microbiomes, much less is known about the functional consequences of this variation, especially in...

Comeault, A. A., Orta, A. H., Fidler, D. B., Nunn, T., Ellison, A. R., Anspach, T. A., Matute, D. R. (2025). Phylogenetic and functional diversity among Drosophila-associated metagenome-assembled genomes. mSystems:e0002725 journals.asm.org/doi/10.1128/...

A chance insight into phosgene toxicity It has long been known that phosgene, a war gas and an industrial reagent, causes intense oxidative stress, but how it does so remains unclear. Here w…

Daplan, E., Rodriguez, E., Lane, N., Turin, L. (2025). A chance insight into phosgene toxicity. Free Radic Biol Med, 238:113-122 www.sciencedirect.com/science/arti...

Gut microbiome and host TOR pathway interact to regulate predator-induced aversive memory in Drosophila melanogaster | PNAS The gut microbiome has emerged as a key factor influencing a wide range of host physiological processes and behaviors, though the mechanisms behind...

Chen, H. Y., Wu, P. S., Li, Z., ...., Chiu, Y., Lee, W. T., Fan, S. Z., Wang, P. Y. (2025). Gut microbiome and host TOR pathway interact to regulate predator-induced aversive memory in Drosophila melanogaster. Proc Natl Acad Sci U S A, 122(25):e2422928122 www.pnas.org/doi/10.1073/...

Yoshino, J., Chiu, A., Morita, T., Yin, C., Tenedini, F. M., Sokabe, T., Emoto, K., Parrish, J. Z. (2025). Heat-off responses of epidermal cells sensitize #Drosophila larvae to noxious inputs. bioRxiv www.biorxiv.org/content/10.1...

A Drosophila ecdysone-deficient model to assess the endocrine disruptor activity of Bisphenol A Bisphenol A (BPA) is a well characterized endocrine disruptor that interferes with the activity of a variety of nuclear receptors. For this reason, BP…

Vega-Cuesta, P., Pulido, D., Abia, D., ...., Fernandez-Freire, P., Peropadre, A., de Celis, J. F. (2025). A Drosophila ecdysone-deficient model to assess the endocrine disruptor activity of Bisphenol A. Ecotoxicol Environ Saf, 300:118483 www.sciencedirect.com/science/arti...

Endosymbiont control through non-canonical immune signaling and gut metabolic remodeling Burgmer et al. show that the pattern recognition receptors (PRRs) PGRP-LC and PGRP-LE repress key metabolic functions in the Drosophila midgut. These regulations occur independently of canonical immun...

Burgmer, S., Meyer Zu Altenschildesche, F., Gyenis, A., Lee, H., Vilchez, D., Giavalisco, P., Fichant, A., Uhlirova, M., Storelli, G. (2025). Endosymbiont control through non-canonical immune signaling and gut metabolic remodeling. Cell Rep, 44(6):115811 www.cell.com/cell-reports...

Kolesnikova, T. D., Veselova, O. V., Pokholkova, G. V., Schubert, V., Klenov, M. S. (2025). Localization of rDNA-associated retrotransposons refines the heterochromatin map of the X chromosome in Drosophila melanogaster. Cytogenet Genome Res:1-20 karger.com/cgr/article-...

Pandey, S., Nguyen, A. T., Maricle, A. K., DiMario, P. J. (2025). Genetic Factors Linking Nucleolar Stress with R2 Retrotransposon Expression in Drosophila melanogaster. Int J Mol Sci, 26(12) www.mdpi.com/1422-0067/26...

An endogenous retroviral element co-opts an upstream regulatory sequence to achieve somatic expression and mobility Abstract. Retrotransposons, multi-copy sequences that propagate via copy-and-paste mechanisms, occupy large portions of eukaryotic genomes. A great majorit

Rubanova, N., Singh, D., Barolle, L., ...., M., Servant, N., Bardin, A. J., Siudeja, K. (2025). An endogenous retroviral element co-opts an upstream regulatory sequence to achieve somatic expression and mobility. Nucleic Acids Res, 53(11) academic.oup.com/nar/article/...

miR-137 targets Myc to regulate growth during eye development Summary: Identification and characterization of miR-137 as a post-transcriptional regulator of Myc that controls growth, offering insights into diseases such as neurodegenerative disorders and cancer,...

adma, R., Subramanian, M., Chimata, A. V., Rai, A., Yogi, S., Sangeeth, A., Kango-Singh, M., Singh, A. (2025). miR-137 targets Myc to regulate growth during eye development. Development journals.biologists.com/dev/article/...

Pianezza, R., Scarpa, A., Haider, A., Signor, S., Kofler, R. (2025). Spatiotemporal Tracking of Three Novel Transposable Element Invasions in Drosophila melanogaster over the Last 30 Years. Mol Biol Evol, 42(7) academic.oup.com/mbe/article/...

Retrotransposon 3S18 forms self-protective aggregates and prolongs mid-oogenesis Transposons are parasitic elements prevalent across nearly all species. Here, Shen et al. tracked the dynamics of a unique retrotransposon in live ovaries, showing that upon activation, this retrotran...

Shen, D., Xu, Y., Shi, Q., Li, C., Meng, Z., Wen, Q., Wang, C., Dou, K. (2025). Retrotransposon 3S18 forms self-protective aggregates and prolongs mid-oogenesis. Cell Rep, 44(7):115914 www.cell.com/cell-reports...

An endogenous retroviral element co-opts an upstream regulatory sequence to achieve somatic expression and mobility Abstract. Retrotransposons, multi-copy sequences that propagate via copy-and-paste mechanisms, occupy large portions of eukaryotic genomes. A great majorit

Rubanova, N., Singh, D., Barolle, L., ...., Bardin, A. J., Siudeja, K. (2025). An endogenous retroviral element co-opts an upstream regulatory sequence to achieve somatic expression and mobility. Nucleic Acids Res, 53(11) academic.oup.com/nar/article/...

miR-137 targets Myc to regulate growth during eye development Summary: Identification and characterization of miR-137 as a post-transcriptional regulator of Myc that controls growth, offering insights into diseases such as neurodegenerative disorders and cancer,...

Padma, R., Subramanian, M., Chimata, A. V., Rai, A., Yogi, S., Sangeeth, A., Kango-Singh, M., Singh, A. (2025). miR-137 targets Myc to regulate growth during eye development. Development journals.biologists.com/dev/article/...

Spatiotemporal Tracking of Three Novel Transposable Element Invasions in Drosophila melanogaster over the Last 30 Years Abstract. Transposable elements (TEs) are repetitive sequences capable of mobilizing within genomes, exerting a significant influence on evolution througho

Pianezza, R., Scarpa, A., Haider, A., Signor, S., Kofler, R. (2025). Spatiotemporal Tracking of Three Novel Transposable Element Invasions in Drosophila melanogaster over the Last 30 Years. Mol Biol Evol, 42(7) academic.oup.com/mbe/article/...

Retrotransposon 3S18 forms self-protective aggregates and prolongs mid-oogenesis Transposons are parasitic elements prevalent across nearly all species. Here, Shen et al. tracked the dynamics of a unique retrotransposon in live ovaries, showing that upon activation, this retrotran...

Shen, D., Xu, Y., Shi, Q., Li, C., Meng, Z., Wen, Q., Wang, C., Dou, K. (2025). Retrotransposon 3S18 forms self-protective aggregates and prolongs mid-oogenesis. Cell Rep, 44(7):115914 www.cell.com/cell-reports...

MLE (DHX9) Helicase Regulates the Expression of Constitutive and Inducible Isoforms of the Conserved Nuclear Receptor FTZ-F1 (NR5A3) - Molecular Biology Abstract In addition to participating in dosage compensation, the MLE helicase in D. melanogaster performs many functions in the regulation of gene expression, as does its human ortholog DHX9. Many of these functions are evolutionarily conserved and poorly explored. MLE has previously been shown to be involved in the regulation of inducible transcription of the ftz-f1 gene encoding the evolutionarily conserved nuclear receptor NR5A3. The ftz-f1 gene also encodes a constitutive transcript synthesized from an alternative promoter. The present work is devoted to the investigation of the role of MLE in the regulation of constitutive transcription of the ftz-f1 gene. This work shows that in S2 cell culture, MLE binds to the constitutive promoter and controls both inducible and constitutive transcription of the ftz-f1 gene. A novel MLE-binding cis-regulatory element of the ftz-f1 gene, enhancer 663, was identified. Using chromosome conformation capture technique the interaction of enhancer 663 with constitutive and inducible promoters of ftz-f1 gene in S2 cell culture was demonstrated. Examination of enhancer 663 histone H3 acetylation showed that it is involved in the activity of both promoters. Knockdown of MLE in S2 cell culture causes an increase in constitutive transcription. The effect of MLE on transcription beyond dosage compensation in vivo at the adult stage was shown for the first time. It was shown that at the adult stage MLE binds to both inducible and constitutive promoters and to enhancer 663. Mutation in the mle gene leads to increased expression of both transcripts of the ftz-f1 gene in females. The data obtained are important for understanding and further study of the evolutionarily conserved functions of MLE and its human ortholog DHX9.

Nikolenko, J. V., Georgieva, S. G. (2025). MLE (DHX9) Helicase Regulates the Expression of Constitutive and Inducible Isoforms of the Conserved Nuclear Receptor FTZ-F1 (NR5A3). Mol Biol (Mosk), 59(2):266-276 link.springer.com/article/10.1...

Dong, P., Li, Y., Wang, Y., Zhao, Q., Lu, T., Chen, J., Guo, T., Ma, J., Yang, B., Wu, H., Huang, H. (2025). Fat body-derived cytokine Upd2 controls disciplined migration of tracheal stem cells in Drosophila. Elife, 13 elifesciences.org/articles/100...

Integrins coordinate basal surface contraction and oriented cell growth to enable thickening of a curved epithelium Lancaster et al. show uniform thickening of a curved epithelium, such as the developing Drosophila retina, is achieved through the coordination of oriented cell growth, which promotes cell elongation,...

Lancaster, C., Manhart, A., Pichaud, F. (2025). Integrins coordinate basal surface contraction and oriented cell growth to enable thickening of a curved epithelium.. Curr Biol, www.cell.com/current-biol...

Roles played by Enhancer of split transcription factors in Drosophila R7 photoreceptor specification When a cell receives multiple developmental signals simultaneously, the intracellular transduction pathways triggered by those signals are coincidenta…

Arias, R. A., Mavromatakis, Y. E., Tomlinson, A. (2025). Roles played by Enhancer of split transcription factors in Drosophila R7 photoreceptor specification. Dev Biol, 526:38-51 www.sciencedirect.com/science/arti...

Chatterjee, M., Yu, X. Y., Brady, N. K., Amendola, C., Hatto, G. C., Reed, R. D. (2025). mirror determines the far posterior domain in butterfly wings. Elife, elifesciences.org/articles/96904

Muscle growth by sarcomere divisions When muscle cells enlarge during developmental growth, they divide their contractile sarcomeres into daughter sarcomeres.

Rodier, C., Estabrook, I. D., Chan, E. H., Rice, G., Loreau, V., Raunser, S., Gorlich, D., Friedrich, B. M., Schnorrer, F. (2025). Muscle growth by sarcomere divisions. Sci Adv, 11(28):eadw9445 www.science.org/doi/10.1126/...

Akarsu, G., MacCharles, K. R., Wong, K. K. L., Richman, J. M., Verheyen, E. M. (2025). Robinow syndrome DVL1 variants disrupt morphogenesis and appendage formation in a Drosophila disease model. Dev Dyn, anatomypubs.onlinelibrary.wiley.com/doi/full/10....

Neuronal glycogen breakdown mitigates tauopathy via pentose-phosphate-pathway-mediated oxidative stress reduction - Nature Metabolism Bar et al. show that neuronal glycogen feeds the pentose phosphate pathway and contributes to redox homeostasis, thus counteracting tauopathy-associated oxidative stress in Drosophila melanogaster.

Bar, S., Wilson, K. A., Hilsabeck, T. A. U., ...., Schilling, B., Ellerby, L. M., Kapahi, P. (2025). Neuronal glycogen breakdown mitigates tauopathy via pentose-phosphate-pathway-mediated oxidative stress reduction. Nat Metab www.nature.com/articles/s42...

Bayar, S., Seep, L., Doubkova, K., ...., Tavosanis, G., Hasenauer, J., Mass, E. (2025). Developmental programming by maternal obesity alters offspring lifespan and immune responses in a diet- and sex-specific manner. Cells Dev:204040 pubmed.ncbi.nlm.nih.gov/40653017/

Mushroom bodies tiny regulates Sidekick localization to tricellular adherens junctions Highlighted Article: Localization of the cell-adhesion molecule Sidekick to tricellular adherens junctions requires specific extracellular domains and its intracellular binding partner, the PAK4 kinas...

Read the article ‘Mushroom bodies tiny regulates Sidekick localization to tricellular adherens junctions’ here: doi.org/10.1242/dev....

Colon cancer cells evade drug action by enhancing drug metabolism - Oncogene Oncogene - Colon cancer cells evade drug action by enhancing drug metabolism

Cong, B., Thakur, T., Uribe, A. H., Stamou, E., Gopinath, S., Sansom, O., Maddocks, O., Cagan, R. (2025). Colon cancer cells evade drug action by enhancing drug metabolism. Oncogene www.nature.com/articles/s41...

Krabbenhoft, S. D., Masuda, T. E., ...., Lewis, P. W., Harrison, M. M. (2025). Investigating oncoprotein-mediated chromatin dysregulation in Drosophila melanogaster uncovers novel modifiers of the developmental impact of H3 K27M and EZHIP. bioRxiv, www.biorxiv.org/content/10.1...

Epigenetic reprogramming induced by key metabolite depletion is an evolutionarily ancient path to tumorigenesis Summary: Human tumors with similar metabolic signatures to fly tumors have lower mutational load. Depletion of key metabolites may be an evolutionarily ancient driving force for tumorigenesis.

Chen, Z., Zhang, X., Deng, M., Li, C., Nguyen, T. T., Liu, M., Dou, K., Ishibashi, T., Wang, J., Yan, Y. (2025). Epigenetic reprogramming induced by key metabolite depletion is an evolutionarily ancient path to tumorigenesis. Dis Model Mech, 18(6) journals.biologists.com/dmm/article/...

Kong, D., Li, X., Zhao, S., ...., Jin, B., He, L., Ma, X. (2025). Adipose tissue-secreted Spz5 promotes distal tumor progression via Toll-6-mediated Hh pathway activation in #Drosophila. www.embopress.org/doi/full/10....