Adam Pavlinek

Early career group leaders We appoint researchers from across biology and biomedicine to set up their first groups at the Crick.

The @crick.ac.uk is recruiting Early Career Group Leaders

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- All areas of biology

Deadline 27 Nov

www.crick.ac.uk/careers-stud...

A very well-written perspective on our understanding of Autism for a lay audience 👇

Facing the possibility of consciousness in human brain organoids The authors challenge recent claims that the emergence of consciousness in human brain organoids (HBOs) remains implausible in the near term. Drawing on neuroscientific evidence, developmental paralle...

Sensationalist claims by certain organoid researchers about the degree of similarity or complexity of neural organoids to the in vivo human brain can lead to profound misunderstandings about the capabilities of organoids by researchers and the lay public, this opinion piece being a prime example.

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AlphaGenome deepmind.google/discover/blo... 🧬🖥️🧪
API: github.com/google-deepm...
Docs: www.alphagenomedocs.com
Community: www.alphagenomecommunity.com

I believe that the current framework is more than sufficient, brain organoids are nowhere near the complexity that would require extensive regulation anytime soon. I think involving the lay public with poor understanding in regulating iPSC work would lead to unnecessary restrictions on research.

I think it’s back up now!

Has NIH Bioart been shut down? 🧪

Revisiting sex and gender in the brain To conduct scientifically accurate and socially responsible research, think of “sex” as a complex, multifactorial and context-dependent variable.

ICYMI: Sex-related factors are important in two ways, writes Marija Kundakovic @kundakoviclab.bsky.social. They are more precise variables than sex, and they are gender-independent. Part of our series on sex differences in the brain.

#neuroskyence

www.thetransmitter.org/sex-differen...

President Trump has slashed funding for medical research, threatening a longstanding alliance between the federal government and universities that helped make the U.S. the world leader in medical science. Here are the nearly 2,500 grants that have been canceled or delayed. https://trib.al/iBstU2B

Brain tissues, assemble! Inside the push to build better brain models Nature - With organoids, assembloids and a growing toolkit of bioengineering tricks, scientists are stitching together models of the developing human brain — and pushing the limits of realism and...

With organoids, assembloids and a growing toolkit of bioengineering tricks, scientists are stitching together models of the developing human brain — and pushing the limits of realism and control

https://go.nature.com/44BRRtC

Astrocyte-secreted cues promote neural maturation and augment activity in human forebrain organoids | Nature Communications Brain organoids have been proposed as suitable human brain model candidates for a variety of applications. However, the lack of appropriate maturation limits the transferability of such functional tools. Here, we present a method to facilitate neuronal maturation by integrating astrocyte-secreted factors into hPSC-derived 2D and 3D neural culture systems. We demonstrate that protein- and nutrient-enriched astrocyte-conditioned medium (ACM) accelerates neuronal differentiation with enlarged neuronal layer and the overproduction of deep-layer cortical neurons. We captured the elevated changes in the functional activity of neuronal networks within ACM-treated organoids using comprehensive electrophysiological recordings. Furthermore, astrocyte-secreted cues can induce lipid droplet accumulation in neural cultures, offering protective effects in neural differentiation to withstand cellular stress. Together, these data indicate the potential of astrocyte secretions to promote neural maturat

Astrocyte cues boost neuron maturity in forebrain organoids, thanks to protein and nutrient-rich medium. From 2D to 3D cultures, neurons mature faster! PMID:40122897, Nat Commun 2025, @NatureComms https://doi.org/10.1038/s41467-025-58295-3 #Medsky #Pharmsky #RNA #ASHG #ESHG 🧪

Cool! Have been playing around with it. Definitely like the protocols.io integration, it has a lot of potential even just for formattting protocols. I think the default auto conversion method uses chatGPT at the moment.

This looks interesting, but no guarantee the information will be accurate - they say “almost” no hallucinations, I wonder what the error rate is compared to standard chatGPT. Also only trained on Springer Nature papers and protocols so missing out on a lot of information.

“Almost” zero hallucinations claimed - what does “almost” mean here? Any stats?

Congrats!

🧪 Check out my thread on our latest brain organoid preprint! Includes some videos of MEA activity! 🎥

Adam PAVLINEK | PostDoc Position | Doctor of Philosophy | King's College London, London | KCL | Research profile Adam PAVLINEK, PostDoc Position | Cited by 62 | of King's College London, London (KCL) | Read 4 publications | Contact Adam PAVLINEK

Hi Monica, would love to be added! I’m a Postdoc that works with brain organoids to study neurodevelopmental conditions and sex differences.
ORCID: 0000-0002-0138-8321
Research gate: www.researchgate.net/profile/Adam...

Thanks!

Thanks Rose! Making figures is my favorite part of paper writing 😁

Dissociated organoids have the benefit of initial 3D development as organoids and have more diverse cell composition compared to monocultures. We think this scalable approach could be applied to high-throughput studies of electrophysiological development! Go check out the preprint! 5/5

We did an in depth-characterization of functional connectivity in these networks using existing analysis tools and also used dimensionality reduction to integrate data across multiple MEA parameters, which can be great for visualizing the effects of treatments or comparing different conditions. 4/5

We analyzed development of activity over time across the different cell lines. The dissociated organoids had unsynchronized activity to begin with, but firing became synchronized across electrodes over time, and this happened across different cell lines. [Warning - video has flashing imagery.] 3/5

We grew unguided human neural organoids from four different ESC and iPSC lines and then dissociated them to 2D cultures that we plated onto micro electrode plates. We then recorded activity over time to characterize these networks and how that vary between lines and batches. 2/5

Excited to share our newest preprint, Electrophysiological development and functional plasticity in dissociated human cerebral #organoids across multiple cell lines 🧠🔬🧪: doi.org/10.1101/2025... This is based on some of the work I did in my PhD with the great @ncndgroup.bsky.social 1/5

Excited to share our latest preprint, presenting a multi-omic human neural organoid cell atlas of the posterior brain! 🧠🔬
doi.org/10.1101/2025...
Great work with @hsiuchuanlin.bsky.social @zhisonghe.bsky.social @graycamplab.bsky.social and Barbara Treutlein!

Neuroscientists have more direct access to brain than ever Model systems continue to offer us tremendous insight, but it’s time for basic researchers to train their sights on the human brain.

Contributing editor Josh Sanes explains why, if he were starting his neuroscience career today, he’d focus on human neurobiology.

www.thetransmitter.org/human-neurot...

In this week’s @nature.com, we publish a group effort to outline a framework for studies with neural #organoids, #assembloids and transplantation. More details below 1/n

Very concerning if this policy is changed. Luckily there is a lot more awareness now of SABV and my hope is more journals will step up and require this + good scientists will continue including females in studies.

Woah does this allow freezing of organoids that can be revived and cultured again?

Human iPSC-derived microglial cells protect neurons from neurodegeneration in long-term cultured adhesion brain organoids - Communications Biology Human iPSC-based modeling using microglia-containing long-term adhesion brain organoids reveals the role of microglia in supporting neuronal growth and function, as well as protecting neurons from neu...

Chen et al. of @cityofhope.bsky.social developed a platform for keeping brain #organoids in culture for >1 year! The new model is abundant in microglia & astrocytes, with great potential for neurodevelopmental & neurodegenerative research. 🧠 In @commsbio.bsky.social
www.nature.com/articles/s42...