Co-translational control of protein stability and quality in plants
Abstract. Proteostasis relies on the coordinated control of protein synthesis, folding, modification and degradation, and an increasingly clear picture is
My review “Co-translational control of protein stability and quality in plants” is now online at @jxbotany.bsky.social, in which I describe how co-translational processing and ribosome-associated quality control together establish protein stability and fate early in synthesis. tinyurl.com/5dhhz9h6
24.02.2026 09:45 —
👍 41
🔁 24
💬 0
📌 0
Indeed! Feel free to suggest here or to ISPT. Also, many speakers will be selected from Abstracts so this route is open.
23.02.2026 10:40 —
👍 0
🔁 0
💬 0
📌 0
Protein Termini 2026 – International Society for Protein Termini (ISPT)
8 days left to register | 28 invited speakers | Keynotes from F. Ulrich Hartl, Roland Beckmann and Michael Rapé | #chaperones #degradation #ubiquitin #cryoEM #acetylation #lipidation #ribosomes #proteins
proteintermini.org/meeting/
23.02.2026 09:16 —
👍 4
🔁 2
💬 1
📌 0
Actin N-terminal maturation can be blocked by a butynamide stereoprobe which acts as an ACTMAP inhibitor. Preprint by Xiong et al. Benjamin Cravatt and Bruno Melillo. @ispt-proteinterm.bsky.social
www.biorxiv.org/content/10.6...
23.02.2026 08:32 —
👍 9
🔁 5
💬 0
📌 0
Join us in Palermo!
FEBS Workshop Protein Termini 2026: the power of protein termini across bacteria, plants, and animals—from ribosome biology to proteostasis and applications. Deadline: 3 March 2026
proteintermini.org/meeting
#ProteinTermini #Proteostasis #FEBS #EMBO @iubmb.bsky.social
16.02.2026 15:10 —
👍 12
🔁 8
💬 1
📌 0
Join us at the @crick.ac.uk for the 2026 meeting of the UK proteostasis community!
We especially encourage students and postdocs to attend and share their work. All talks (except the keynotes) will be selected from abstracts.
13.02.2026 13:55 —
👍 19
🔁 15
💬 0
📌 0
Surprisingly: "Conditional stability of HY5 through the ATE N-degron pathway regulates environmental responses in Arabidopsis thaliana".
The shining bounds of N-degron pathway influence expands! @charlene-kunaka.bsky.social www.biorxiv.org/content/10.6...
12.02.2026 08:58 —
👍 15
🔁 14
💬 1
📌 0
Protein event of the year - sign up today! See you at beautiful Palazzo dei Normanni in Palermo for the FEBS 2026 Protein Termini Workshop.
#ProteinTermini #Proteostasis #ProteinModifications #StructuralBiology #PalazzoDeiNormanni #Palermo2026
proteintermini.org/meeting/
09.02.2026 20:49 —
👍 8
🔁 8
💬 1
📌 1
FEBS Workshop 'Protein termini 2026: From mechanisms to biological impact'
Remember to register for this year's Protein Termini FEBS Advanced course. Keynote by Roland Beckmann @beckmannlab.bsky.social "It takes more than two to tango: structural basis and coordination of co-translational N-terminal nascent chain modification" www.conferencecentral.org/webpage/view...
07.02.2026 15:49 —
👍 3
🔁 2
💬 0
📌 0
Functional divergence of the Arg/N-degron pathway between the crop Brassica rapa and the model plant Arabidopsis thaliana
The ubiquitin-dependent Arg/N-degron pathway relates the stability of a substrate protein to the nature of its N-terminal amino acid residue or its biochemical modifications, with some N-terminal residues being recognized by specific E3 ubiquitin ligases, resulting in the ubiquitylation and degradation of the substrate protein. Work in the model plant Arabidopsis thaliana has shown that the Arg/N-degron pathway is a key regulator of plant responses to hypoxia, which can be either physiological or a stress in the context of waterlogging or submergence. The role of the Arg/N-degron pathway in hypoxia response is mediated via the oxygen-dependent degradation of group VII ETHYLENE RESPONSE FACTOR (ERFVII) transcription factors, which act as the master regulators of the hypoxia response program in plants. Analysis of Arabidopsis mutants for different enzymatic components of the Arg/N-degron pathway has also revealed its roles in the regulation of responses to other abiotic stresses (e.g. salt stress), as well as to pathogens. Although much has been learned from studies in Arabidopsis about the functions of the Arg/N-degron pathway, very little is known about this pathway in crops, including in Brassica crops such as oilseed rape, cabbage or turnip. To determine functional similarities and divergence of the Arg/N-degron pathway between Arabidopsis and Brassica crops, we isolated and characterized the first Arg/N-degron pathway mutants in Brassica rapa (turnip, pak choi), a diploid Brassica crop closely related to oilseed rape. We focused on two enzymatic components, namely the arginine-transferases ( ATE s) and the E3 ubiquitin ligase PROTEOLYSIS6 ( PRT6 ). Our results show both similarities and divergence of function for these Arg/N-degron pathway components in B. rapa compared to Arabidopsis. Specifically, ATE mutants in B. rapa arrest their development at the seedling stage, which contrasts with the mild phenotypic defects of the equivalent Arabidopsis mutants. Double mutant lines for two of the three PRT6 genes in B. rapa indicated a constitutive activation of hypoxia response genes at the transcriptional level, as shown in the single prt6 mutant in Arabidopsis. However, contrary to Arabidopsis, the B. rapa double mutants were more sensitive to waterlogging and hypoxia, and did not show differential response to salt stress or to biotic stress compared to the wild type. The functional divergence identified likely reflects variability in each species in the substrate repertoire and/or in the regulation of pathways or targets downstream of Arg/N-degron pathway substrates. Such differences could be driven by direct selective pressures at N-termini (e.g. gain or loss of a destabilizing N-terminal residue), or by species-specific proteases that may generate destabilizing neo-N-termini after cleavage. These similarities and differences highlight the difficulties in translating research findings from Arabidopsis to crops, even within the same plant family (Brassicaceae) and highlight the need to study pathways in crops. ### Competing Interest Statement The authors have declared no competing interest. Science Foundation Ireland, https://ror.org/0271asj38, 13/IA/1870, 20/FFP-P/8433 Irish Research Council, https://ror.org/051xex213, GOIPG/2017/2
Protein degradation in Turnip - an adapted Arg/N-degron pathway. Preprint from Brian Mooney et al. Emmanuelle Graciet lab. www.biorxiv.org/content/10.6...
30.01.2026 14:09 —
👍 6
🔁 5
💬 0
📌 0
Complexity at the ribosomal polypeptide tunnel exit. The major N-terminal acetyltransferase NatA may form different complexes on the ribosome to facilitate protein maturation. By Marius Klein & Klemens Wild in Irmgard Sinning's lab BZH & Nina McTiernan in my lab. www.nature.com/articles/s41...
26.01.2026 11:24 —
👍 16
🔁 8
💬 0
📌 1
This is how co-translational N-terminal myristoylation occurs on the ribosome. NMT1 acts together with NAC, but after the release of MetAP. Cryo-EM study by Timo Denk et al. @beckmannlab.bsky.social @giglionelab.bsky.social www.nature.com/articles/s41...
26.01.2026 11:00 —
👍 17
🔁 6
💬 0
📌 0
🎉 As we celebrate #TIBS50, we're highlighting #CitedClassics and revisiting the top cited article from each of the last 50 years!
Check out this one from 1976! Read it here 👉 lnkd.in/egdN-AXt
13.01.2026 16:45 —
👍 3
🔁 1
💬 1
📌 1
1. Apply for one of the positions below (lab or theory) by Jan 18th
2. Learn interdisc skills and discover cool new things about how mitochondria move and socialise
3. Explore some of Norway's beautiful nature (both the below, Rundemanen and Gullfjellet, <10km from work)
09.01.2026 08:02 —
👍 5
🔁 8
💬 0
📌 0
Mechanism of cotranslational modification of histones H2A and H4 by MetAP1 and NatD
NAC enables cotranslational N-terminal processing of histones H2A and H4 by recruiting MetAP1 and NatD at the ribosomal tunnel.
Last X-Mas, the ribosome gave you methionine,
but the very next day, MetAP took it away.
This year, to save histones from tears,
NatD gives you an acetyl group. ⭐️
Explore our latest paper with the Deuerling lab @uni-konstanz.de and Shu-ou Chan lab @caltech.edu!
www.science.org/doi/10.1126/...
20.12.2025 00:34 —
👍 46
🔁 17
💬 0
📌 0
📢 Our December issue is now online! Access the full issue 👉 www.cell.com/trends/bioch...
🎨 The cover brings warm, summertime vibes ☀️ and highlights a Feature Review on #AromaticAminoAcid biosynthesis in 🌱 from Jorge El-Azaz and @hiroshi-maeda.bsky.social. Cover art credit to Tae Park.
(1/n)
04.12.2025 17:59 —
👍 11
🔁 2
💬 1
📌 2
We’ve made some new tools to manipulate N-recognins. Check them out in our preprint.
www.biorxiv.org/content/10.1...
29.11.2025 03:24 —
👍 18
🔁 13
💬 0
📌 0
FEBS Workshop 'Protein termini 2026: From mechanisms to biological impact'
Just registered for FEBS advanced workshop Protein termini 2026 in Palermo #proteintermini2026 (?) Looking forward to a great meeting. @ispt-proteinterm.bsky.social
www.conferencecentral.org/webpage/view...
03.12.2025 14:05 —
👍 2
🔁 4
💬 0
📌 0
Our study led by the extraordinary multitasking Daai Zhang shows here (tinyurl.com/3uhbkh54) that an additional O2 sensing mechanism based on histone methylation helps roots to prepare for potentially lethal hypoxic stress (such as in waterlogging)
27.11.2025 10:45 —
👍 21
🔁 8
💬 1
📌 2
📝 Online now - the Review "Emerging functions for nonhistone protein acetylation in budding yeast" from Michael Downey and colleagues.
#PostTranslationalModifications #DNADamageResponse #Proteostasis #Autophagy #MetabolicRegulation
Read it here: authors.elsevier.com/a/1m4nM3S6Gf...
12.11.2025 14:28 —
👍 3
🔁 1
💬 0
📌 0
Want to assess N-terminal acetyltransferases without radioactive labelling or mass spec? Look here! @ispt-proteinterm.bsky.social @cellsignal.com
27.10.2025 20:25 —
👍 4
🔁 3
💬 1
📌 0
See you at Palazzo dei Normanni in Palermo for the 2026 Protein Termini Conference | Co- and post-translational modifications | Proteolytic processing and degradation pathways | Microbes, plants, animals #ProteinTermini2026 #Proteostasis #ProteinModifications #PalazzoDeiNormanni #Palermo2026
26.10.2025 18:59 —
👍 7
🔁 7
💬 2
📌 1
Daniel Gibbs Lab
Welcome to the lab website of Professor Daniel Gibbs
@ University of Birmingham
We are advertising a PhD project investigating a cellular mechanism that ensures protein quality control during mRNA translation in plants. Please get in touch on here or via email if you are interested and I can provide further details!
Lab website: sites.google.com/site/danielg...
14.10.2025 14:07 —
👍 38
🔁 41
💬 0
📌 0
📢 Our October issue is now online. Access the full issue 👉 www.cell.com/trends/bioch....
🎨 The cover highlights a Review from @chiosislabmskcc.bsky.social and co on how PTMs reprogram chaperones into epichaperomes.
06.10.2025 14:40 —
👍 7
🔁 4
💬 1
📌 1
