Thomas Arnesen

NAA60 facilitates LRRC8A- and LRRC8D-mediated platinum drug uptake - Communications Biology Genome-wide CRISPR/Cas9 screening identifies the N-terminal acetyltransferase NAA60 to facilitate cis- and carboplatin drug sensitivity, most likely by neutralizing Nt acetylation of the LRRC8A/D VRAC subunits regulating substrate permeability.

Uptake of anti-cancer drugs depends on N-terminally acetylated anion channels | @ispt-proteinterm.bsky.social www.nature.com/articles/s42...

📢 Our October issue is now online. Access the full issue 👉 www.cell.com/trends/bioch....

🎨 The cover highlights a Review from @chiosislabmskcc.bsky.social‬ and co on how PTMs reprogram chaperones into epichaperomes.

And now for something completely different in co-translational targeting In two recent papers in Cell, Zhu et al. and Luo et al. use selective ribosome profiling to identify the substrates and timing of co-translational protein targeting to mitochondria. The results sugges...

And now for something completely different in co-translational targeting: Molecular Cell www.cell.com/molecular-ce...

A Cell-Potent Bisubstrate Inhibitor to Probe NatD Acetyltransferase Activity N-terminal acetyltransferase D (NatD) is a highly selective enzyme that acetylates the α-N-terminal amine of histones H4 and H2A, which share the SGRGK motif. Elevated NatD expression has been observe...

A Cell-Potent Bisubstrate Inhibitor to Probe NatD Acetyltransferase Activity | The inhibitor effectively reduced cellular Nα-acetylation on histone H4, leading to reduced migration in lung cancer cells | @acs.org ACS Chemical Biology @ispt-proteinterm.bsky.social pubs.acs.org/doi/10.1021/...

Protein N-terminal acetylation regulates the growth of rice plants | #NAA30 #NatC #rice #acetylation www.sciencedirect.com/science/arti...

Two new volumes of Methods in Enzymology with 29 chapters/papers on Protein termini methods and reviews are now out! Thx to all authors!🙌 @ispt-proteinterm.bsky.social #N-degron #prenylation #myristoylation #formylation #arginylation #oxidation #acetylation www.sciencedirect.com/bookseries/m...

Are you excited?? Because I am!

25 years ago I was 10 and had just started secondary school yet our journal was already covering groundbreaking science, long before I'd even heard of molecular cell biology 🧐
Safe to say I'm fairly familiar with it now 🤭

Enjoy our issue!!!!!!!!!!!

Developing leaves form a spatiotemporal oxygen gradient that is sensed through the oxygen sensing machinery and directs morphogenesis | New preprint from @daanweits.bsky.social and @syno2xis.bsky.social labs #N-degron #Cysteine #hypoxia @ispt-proteinterm.bsky.social www.biorxiv.org/content/10.1...

Anatomy and habitat shape the oxygen sensing machinery of angiosperms Hypoxia sensing via the Cys/Arg branch of the N-degron pathway (Cys-NDP) is central for flooding responses in plants, yet how evolutionary and ecological factors have shaped the core oxygen sensing me...

Anatomy and habitat shape the oxygen sensing machinery of flowering plants | new preprint from Licausi lab @syno2xis.bsky.social #hypoxia #N-degron @ispt-proteinterm.bsky.social www.biorxiv.org/content/10.1...

The generation and consequences of N-terminal proteoform diversity N-terminal proteoforms are protein variants with altered N termini that arise from RNA-driven processes, such as alternative promoter usage, splicing,…

New review on the generation and consequences of N-terminal proteoform diversity from Evan Morrison and Olivia Rissland | #N-degron #RNA @cp-cellreports.bsky.social www.sciencedirect.com/science/arti...

🎉 As we prep for #TIBSCelebrates50, I'd love for participation from YOU! Share your creativity and have your artwork featured on the cover of @cp-trendsbiochem.bsky.social!

📆 Deadline is Sept 30!!

Targeted degradation of pathogenic TDP-43 proteins in amyotrophic lateral sclerosis using the AUTOTAC platform Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease characterized by the progressive loss of motor neurons and the cytoplasmic aggregation of misfolded proteins in the spinal cord...

Targeted degradation of pathogenic TDP-43 proteins in amyotrophic lateral sclerosis using the AUTOTAC platform | Yong Tae Kwon lab | #N-degron www.biorxiv.org/content/10.1...

A synthetic ERFVII-dependent circuit in yeast sheds light on the regulation of early hypoxic responses of plants Plants face hypoxic conditions either chronically, as particular tissues are characterized by fluctuating or stable low oxygen levels, or acutely, when flooded. In vascular plants, transcriptional ada...

A synthetic ERFVII-dependent circuit in yeast sheds light on the regulation of early hypoxic responses of plants | Preprint from @mikellavi.bsky.social @beagiuntoli.bsky.social @syno2xis.bsky.social #N-degron #hypoxia www.biorxiv.org/content/10.1...

Glad to share the final version of our story about the UBR4 complex, an E4 ligase protein quality control hub @science.org. Now with more cryo-EM structures and a deeper dive into substrate recognition, especially escaped mitochondrial proteins @clausenlab.bsky.social www.science.org/doi/10.1126/...

Co-translational ribosome pairing enables native assembly of misfolding-prone subunits - Nature Communications Protein complexes are essential for cell function. Here, authors show that paired ribosomes can help each other’s nascent chains fold correctly, enabling proper dimer assembly and preventing misfoldin...

Very cool stuff on the co-translational folding of dimers by Sander Tans and his team @amolf-nl.bsky.social:

www.nature.com/articles/s41...

Want to know how lipidation of some nascent chains takes place by NMT2-- Check our latest work on how NAC couples Protein Synthesis with Nascent Polypeptide Myristoylation on the Ribosome out today @embojournal.org‬: www.embopress.org/doi/full/10....

Structural basis for the recognition and ubiquitylation of type-2 N-degron substrate by PRT1 plant N-recognin
nature.com/articles/s41...
#plantscience

Principles of cotranslational mitochondrial protein import Selective ribosome profiling reveals that nearly 20% of mitochondrial proteins in human cells are imported during translation on cytosolic ribosomes. Cotranslational import requires an N-terminal pres...

Emmanuel Levy @elevylab.bsky.social has joined BlueSky 🌟 with a fantastic Cell paper with Shu-ou Shan, showing the interactome of the TOM complex and how cotranslational mito import prioritizes large globular domains. Beautiful science!
Give him a warm welcome 🎉
link: www.cell.com/cell/fulltex...

Nitric oxide promotes cysteine N-degron proteolysis through control of oxygen availability | PNAS Selected proteins containing an N-terminal cysteine (Nt-Cys) are subjected to rapid, O2-dependent proteolysis via the Cys/Arg-branch of the N-degro...

Nitric oxide promotes cysteine N-degron proteolysis through control of oxygen availability | Important finding by Kim, Tian, Ratcliffe and Keeley @ox.ac.uk @ispt-proteinterm.bsky.social #N-degron www.pnas.org/doi/10.1073/...

Gene Editing of a Susceptibility LncRNA Enhances Broad‐Spectrum Disease Resistance in Rice without Developmental Trade‐Offs Editing susceptibility lncRNAs offers a strategy to enhance disease resistance without yield loss. RESIS, a domestication-acquired, pathogen-induced lncRNA, promotes protein N-terminal acetylation th...

Disease resistance in rice steered by a long non-coding RNA via protein N-terminal acetylation | @ispt-proteinterm.bsky.social advanced.onlinelibrary.wiley.com/doi/10.1002/...

Fine mapping and prediction of a candidate gene for wrinkled rind in melon (Cucumis melo L.) - Theoretical and Applied Genetics Key message The Cmwr locus was fine mapped to chromosome 2 in wrinkled-rind melon, and MELO3C010304.2 (CmNaa35, encoding an N-α-acetyltransferase) was identified as the most likely candidate gene for ...

Melon wrinkles linked to protein N-terminal acetylation. link.springer.com/article/10.1...

Global profiling of N-terminal cysteine–dependent degradation mechanisms | PNAS Hypoxia, a condition characterized by insufficient oxygen supply, challenges cellular homeostasis and energy production, triggering adaptive respon...

Global profiling of N-terminal cysteine–dependent degradation mechanisms | PNAS | @ispt-proteinterm.bsky.social www.pnas.org/doi/10.1073/...

Repost appreciated:
Open #PhDposition in structural virology in my group! Join us in Umeå, Sweden to uncover how arboviruses remodel infected cells. In situ #cryoET and #cryoEM combined with #virology, #cellbiology, and #biophysics.
Deadline 7 September. More info: www.carlsonlab.se/join/

💡Interested in our recently advertised @embl.org-style group leader positions but still not sure about it? Maybe this clip will convince you to join us👇!

The evolutionary rise of oxygen sensing in land plants Land plants evolved from aquatic green algal ancestors to dominate terrestrial ecosystems approximately 480 million years ago during the Ordovician period (Hammarlund et al., 2020). This major event i...

Our 'Spotlight' discussing an excellent recent paper by @lauradc.bsky.social @n-end-rules.bsky.social & @syno2xis.bsky.social et al on the evolution of oxygen signalling in land plants is now online at Molecular Plant.

Spotlight: tinyurl.com/7ydn6p4a

Original paper: tinyurl.com/yw5yd4f8

📢 Our August issue is online!

🎨 The cover highlights a Feature Review from Stephanie Rosswag de Souza, @gzaffagnini.bsky.social, and @bokelab.bsky.social on how proteostasis functions in cellular dormancy across a number of examples, from yeast to adult quiescent stem cells and vertebrate oocytes.

Zebrafish model shows that NAA15 and N-terminal acetylation defects cause heart disease | Independently confirms the causal link between pathogenic NAA15 variants and congenital heart disease | Burns lab @bostonchildrens.bsky.social www.biorxiv.org/content/10.1...

Mechanism for voltage-gated channel inactivation uncovered | N-terminal acetylation of the intracellular domain of the Shaker potassium channel is key for plugging the pore | By Kenton Swartz lab in @nature.com @ispt-proteinterm.bsky.social www.nature.com/articles/s41...

How to define degrons and E3 ligase specificities by peptide pulldowns | Methods in Enzymology | Kwon and Ji labs | #KCMF1 #Cysteine #Arg/N-degron @ispt-proteinterm.bsky.social www.sciencedirect.com/science/arti...

Using a specific antibody to detect proteins carrying N-terminal formylation | Methods in Enzymology | Hwang lab @ispt-proteinterm.bsky.social www.sciencedirect.com/science/arti...