Artem Minin

A 3D-Printed Compliant Micro-Manipulator - XYZ Positioning down to 1µm YouTube video by Diffraction Limited

An awesome thing that YouTube recommended to me. Partially printed 3D stage with micron precision. Partially: the flexure member is made of metal string.

www.youtube.com/watch?app=de...

github.com/0x23/MicroMa...

#DIY #Microscopy

Or is this deliberate trolling of Trump by soldiers who definitely don't want to be here.

Well, since you've read this far, we're already quite familiar with each other. So I'm ready to introduce you not only to spheroids, but also to sausageoids and 3d_cell_culture of ... other shapes.

I was greatly inspired to start this project by
@vsaggiomo.bsky.social, who has a whole series of such DIY projects. @senger.bsky.social and @alunellab also expressed interest at various stages.

Thank you.

8/n

The work turned out to be as open source as possible, all models were made in
@freecadnews.bsky.social, microscope images were processed in napari, ImageJ and CellProfiler

7/n

If you have a logical question: does the shape approximated by voxels produced by printers interfere with the cells? No, it does not interfere. I was also surprised, but the spheroids form perfectly.

Blue in the video is the CAD model, red is in the slicer, green is what actually comes out.

6/n

I tried to make everything as accessible as possible and specifically checked the reproducibility by distributing 3D models and printed samples of stamps to biologists I know. Everyone succeeded.

Models on GitHub can also be modified to suit specific tasks.

5/n

The required feature size for manufacturing spheroids is in the order of hundreds of micrometres, which is quite realistic for an MSLA printer. I mainly used an old Anycubic Mono, but other printers work just as well.

4/n

The MSLASpheroidStamp project includes 3D printed stamps that can be used to make indentations in agarose for #cell_culture #spheroids directly, and silicone molds (similar to MicroTissue).

3/n

You will need an MSLA 3D printer (around 200–400 USD), molding silicone, and a corner in the laboratory that will get dirty.

The article was published in Bioprinting: tinyurl.com/yjvuj64u
All protocols and models are available on GitHub:
tinyurl.com/3tm69k75

2/n

So, I'm on holiday, so it's the perfect time to post a thread that's been sitting around for a while.

How difficult is it to make your own spheroid production system comparable to the expensive MicroTissue or AggreWell systems?

Easy, actually.

1/n

#DIY #biology #science #3d_printing

I've tried a lot of options. It's not reliable and effective enough, so I'm just going to have to live with it.

Plant Biology Exploratory Research Microgrant | Experiment For Science!

I am ***absurdly*** excited to announce the launch of a new funding opportunity through Experiment dot com!!! You have one month to pitch something wonderful in the realm of plant biology/biotech research and education. $10,000 max grant. PLEASE RE-POST + SHARE!!! 1/3

experiment.com/programs/pla...

Hi! My name is Joyce and I love building and using tools to manipulate the #cytoskeleton of cells to better understand their function. 🔬
Below you can see my movie of #opto-katanin ✂️(SspB-mCh-p60) being used to locally disassemble #microtubules (labelled by SiR-tubulin) inside a neuron.

Was flagged on pubpeer, but I think for nothing! Great article and a brilliant scientific breakthrough!

www.sciencedirect.com/science/arti...

BlueSky Science-Related Feeds

New to Bluesky and looking for science feeds to follow? Check out this list created and updated by @markrubin.bsky.social. Thanks Mark!
docs.google.com/spreadsheets...

Axons ❤️ ECM

Let the first post here be a teaser for a paper I'm going to submit to a journal pretty soon. Redundant #DIY, #MSLA 3d printing and #3d_cell_cultures