@jchodera.bsky.social :)

20.06.2025 19:16 — 👍 0    🔁 0    💬 1    📌 0

🧬 𝐊𝐑𝐀𝐒 𝐁𝐢𝐧𝐝𝐢𝐧𝐠: 𝐀 𝐒𝐭𝐞𝐩 𝐓𝐨𝐰𝐚𝐫𝐝 𝐒𝐦𝐚𝐫𝐭𝐞𝐫 𝐈𝐧𝐡𝐢𝐛𝐢𝐭𝐨𝐫𝐬
Different KRAS mutations shape the accessibility of the #switch-II pocket—a key target for modern small-molecule inhibitors.
www.linkedin.com/posts/parenp...

20.06.2025 19:15 — 👍 0    🔁 0    💬 1    📌 0

𝐈𝐃-𝐓𝐞𝐬𝐭𝐢𝐧𝐠 𝐁𝐢𝐨𝐥𝐨𝐠𝐢𝐜𝐬 𝐌𝐚𝐧𝐮𝐟𝐚𝐜𝐭𝐮𝐫𝐢𝐧𝐠 - 𝑭𝒊𝒍𝒍 & 𝑭𝒊𝒏𝒊𝒔𝒉
See how Dr Marius Müller (Johnson & Johnson Innovative Medicine) employs unfolding curves like a fingerprint to confirm product identity.

Sign up here---> lnkd.in/evyFxgSJ
More here tinyurl.com/yc5z5wmf

#Biomanufacturing #Formulation #Biologics

05.03.2025 21:15 — 👍 0    🔁 0    💬 0    📌 0

Thank you @gwendolynawu.bsky.social What a breath of fresh air :)

20.02.2025 20:45 — 👍 0    🔁 0    💬 0    📌 0

@jchodera.bsky.social
IF you were able to generate 1536 data points for in-solution binding affinity in <10 minutes, what would be the impact on "fine tuning" of biomolecular simulation models?? :)

20.02.2025 20:34 — 👍 2    🔁 0    💬 1    📌 0

There's a new toy in the lab! I’ve been dreaming of acquiring a Prometheus for a long time (since my PhD), and we finally managed to purchase one at the #LaJollaInstitute. I can't wait to start characterizing our glycoproteins with it! #biophysics #cryoem #proteinfolding

17.11.2024 06:52 — 👍 35    🔁 2    💬 2    📌 0

A microscale thermophoresis-based enzymatic RNA methyltransferase assay enables the discovery of DNMT2 inhibitors Communications Chemistry, Published online: 03 February 2025; doi:10.1038/s42004-025-01439-9RNA methyltransferases (MTases) are drug targets that catalyze RNA methylation, a reaction associated to disease, but medium and high-throughput screening assays…

Just out: A microscale thermophoresis-based enzymatic RNA methyltransferase assay enables the discovery of DNMT2 inhibitors

04.02.2025 03:13 — 👍 5    🔁 1    💬 0    📌 0

Figure 5. Elongator is a tubulin polymerase selective for polyglutamylated monomers. (A, B) Elp456 binds preferentially to polyglutamylated tubulin. (A) Affinity measurement between eGFP-Elp456 and indicated αβ-tubulin heterodimers using microscale thermophoresis. Estimated dissociation constant (Kd) values are indicated (mean ± s.d.; n = 3). (B) Estimated dissociation constants for the indicated tubulins. Elp456 binds with similar affinity to recombinant Drosophila α1β1-tubulin, pig brain tubulin, mouse brain tubulin and mouse brain tubulin lacking K40 acetylation. However, the binding to tubulin purified from TTLL1/7-double KO mouse brains (lacking polyglutamylation) and HeLa S3 cells (lacking polyglutamylation, K40 acetylation and Δ2 deletion is similar and ~18 times lower than other tubulins. (C) Experimental assay to detect polyglutamylation in polymerized microtubules in vitro. (D) Microtubules polymerized in the presence of Elongator are significantly enriched in polyglutamylated tubulin when using a 50:50 pig brain tubulin:HeLa S3 tubulin mix. Correspondingly, when using HeLa S3 tubulin, which is not polyglutamylated, no effect is observed. P values for an Ordinary one-way ANOVA test followed by two-tailed Turkey multiple comparison test are indicated (E–G) Elongator depletion results in decreased polyglutamylation levels at the spindle. mean ± standard deviation. P values for an Ordinary one-way ANOVA test on independent experiments (N = 3) followed by two-tailed Turkey multiple comparison test are indicated. (E) Drosophila S2 cells depleted for Elongator have significantly decrease levels of polyglutamylated tubulin at the mitotic spindle.

During revisions, we've added lots of exciting new data, so be sure to check out the paper! Most importantly, we now demonstrate that Elongator regulates polyglutamylation levels of spindle microtubules, both in cells and in vivo.

17.01.2025 09:46 — 👍 5    🔁 1    💬 0    📌 0

Prometheus Panta dramatic angle photo

Nanotemper #Prometheus looks so cool 😎

19.11.2024 23:07 — 👍 8    🔁 1    💬 1    📌 0

I’m a standing member on the Chemical Biology and Probes (CBP) NIH study section that was supposed to meet tomorrow. It just got postponed this afternoon with a date yet to be determined….

19.02.2025 20:22 — 👍 23    🔁 12    💬 3    📌 1

𝐀 𝐍𝐞𝐰 𝐄𝐫𝐚 𝐢𝐧 𝐓𝐚𝐫𝐠𝐞𝐭𝐞𝐝 𝐏𝐫𝐨𝐭𝐞𝐢𝐧 𝐃𝐞𝐠𝐫𝐚𝐝𝐚𝐭𝐢𝐨𝐧
𝐂𝐲𝐜𝐥𝐢𝐧 𝐊 - 𝑯𝒐𝒘 𝒂𝒏 𝒊𝒏𝒉𝒊𝒃𝒊𝒕𝒐𝒓 𝒘𝒂𝒔 𝒕𝒓𝒂𝒏𝒔𝒇𝒐𝒓𝒎𝒆𝒅 𝒊𝒏𝒕𝒐 𝒂 𝒎𝒐𝒍𝒆𝒄𝒖𝒍𝒂𝒓 𝒈𝒍𝒖𝒆

tinyurl.com/3khpakrs

19.02.2025 16:59 — 👍 3    🔁 0    💬 0    📌 0