Nathan Fraikin

Expanding the genetic toolbox of the obligate predatory bacterium Bdellovibrio bacteriovorus with inducible gene expression and CRISPR interference Inducible gene expression and CRISPR interference-mediated depletion in the predatory bacterium Bdellovibrio bacteriovorus.

🚨New paper out! #MicroSky
Studying obligate predators like Bdellovibrio bacteriovorus is tricky—essential genes for predation are also essential for survival.
We expanded its genetic toolbox:
🧬promoters to fine-tune expression
🧬IPTG-inducible system
🧬CRISPRi for rapid knockdown
bit.ly/46GUn2c
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Exclusion systems preserve host cell homeostasis and fitness, ensuring successful dissemination of conjugative plasmids and associated resistance genes Abstract. Plasmid conjugation is a major driver of antibiotic resistance dissemination in bacteria. In addition to genes required for transfer and maintena

Exclusion systems, far from slowing down plasmid spread, are in fact essential to preserve host cell viability and thereby ensure the successful dissemination of conjugative plasmids and antibiotic resistance genes.

@nfrk92.bsky.social
@narjournal.bsky.social

academic.oup.com/nar/article/...

Biofilm architecture determines the dissemination of conjugative plasmids | PNAS Plasmid conjugation is a contact-dependent horizontal gene transfer mechanism that significantly contributes to the dissemination of antibiotic res...

Biofilm architecture determines the dissemination of conjugative plasmids: We measured the spatiotemporal spread of plasmids in biofilms and found that plasmids only spread in some biofilm regions!

Collab with lab of @sbigot.bsky.social & Christian Lesterlin
www.pnas.org/doi/10.1073/...

And a complete heresy to mix it in cocktails !

www.addgene.org/browse/artic... (Available from Addgene @addgene.bsky.social )

A palette of bright and photostable monomeric fluorescent proteins for bacterial time-lapse imaging Bright and photostable monomeric fluorescent proteins enable long-term time-lapse imaging in E. coli.

Our set of new prokaryote-optimized fluorescent proteins is now published ! #microbiology #microsky #microscopy 🧫🦠🔬
www.science.org/doi/10.1126/...

MMSB - Molecular Microbiology and Structural Biochemistry @mmsb-lyon.bsky.social is now on Bluesky!
👉 Follow us to know about our last publications, prizes, job offers, seminars, events,...

Something with 1-10 mM Mg2+

WHAAAAAT, we do !!!

New preprint from the lab!! Check it out!

Genetic Determinants of pOXA-48 Plasmid Maintenance and Propagation in Escherichia coli Conjugative plasmids are the main drivers of antibiotic resistance dissemination contributing to the emergence and extensive spread of multidrug resistance clinical bacterial pathogens. pOXA-48 plasmi...

New preprint from our Lesterlin Lab! Yannick Baffert and @nfrk92.bsky.social, in collaboration with @macerwan.bsky.social and @val-meve.bsky.social, shed light on the molecular mechanisms driving pOXA-48 plasmid maintenance and transfer. We reveal novel TA system, regulators, and T4SS components!

which recombination pathway and which species are we talking about ?

a man in a lab coat is sitting at a desk with the words moi je suis une star des maladies infectieuses written below him ALT: a man in a lab coat is sitting at a desk with the words moi je suis une star des maladies infectieuses written below him

fr.wiktionary.org/wiki/Raoulte...

A great paradigm shift for the proximal-distal expression gradient in (super)integrons. Bravo Paula, Jose & team !

RIP Pierre. You were one of the best. Thank you for all you did for our field but more importantly, for me and all the people you helped. I will miss you. ❤️

Gram-pos envelope is like a thick layer of homogenous porous material while gram-neg is more like a composite that keeps all kind of stuff out of the cell. My money's on gram-neg.

weapons allowed ?