Reyes-Lamothe lab

Now in @mdpiopenaccess.bsky.social Bacillus subtilis DinG/XPD-like paralogues, DinG and YpvA, have been implicated in overcoming replication stress. DinG possesses a DEDD exonuclease and DNA helicase domains, whereas YpvA lacks the DEDD exonuclease domain

www.mdpi.com/1422-0067/26...

Dynamics and interplay of gene expression and chromosome organization across a predatory lifecycle The obligate predatory bacterium Bdellovibrio bacteriovorus alternates between a motile attack phase and a growth phase inside another bacterium, during which it undergoes multiple rounds of DNA repli...

📝New preprint! #MicroSky

🕰️We chart the full, synchronized lifecycle of a predatory bacterium using Hi-C, RNA-seq, ChIP-seq & microscopy.

🧬We uncover dramatic shifts in nucleoid architecture & transcription, tightly coordinated with predation.

www.biorxiv.org/content/10.1...

Engineering Spatial Control of Bacterial Organelles Bacteria were once thought to lack organelles, but it is now clear they confine cellular reactions using an array of membrane- and protein-based compartments. A central question, however, is how bacte...

New PrePrint from the Vecchiarelli and Mosalaganti (@shyamalm.bsky.social) labs! 🧵

Collaboration with the Giessen and Pi labs

Congratulations to co-first authors Y Hoang (@hoanghamyy.bsky.social) and Pankaj Jadhav!

www.biorxiv.org/content/10.1...

The eukaryotic replisome intrinsically generates asymmetric daughter chromatin fibers DNA replication is molecularly asymmetric, due to distinct mechanisms for lagging and leading strand DNA synthesis. Whether chromatin assembly on newly replicated strands is also asymmetric remains un...

Some (+)ve news to lighten another heavy weekend: our latest preprint (c/o Mattiroli + Ramani labs) is up!
www.biorxiv.org/content/10.1...
A tour-de-force by 1st authors Bruna Eckhardt & @palindromephd.bsky.social, focusing on chromatin replication. RTs welcome; tweetorial in 3,2...(1/n)

A guide to single-particle tracking - Nature Reviews Methods Primers Single-particle tracking is a method for analysing different motion behaviours of individual proteins and protein complexes in live cells. This Primer outlines the experimental setup and labelling tec...

Really enjoying reading this primer on single particle tracking #microscopy by @svanteeffelen.bsky.social and colleagues www.nature.com/articles/s43... (pdf on Sven's website)

New paper out! We show that RecBC remodels single-stranded DNA gaps to protect them from ExoI (sbcB) 3′-5′ exonuclease degradation during replication of damaged DNA.

doi.org/10.1073/pnas...

The B. subtilis replicative polymerases bind the sliding clamp with different strengths to tune their activity in DNA replication Abstract. Ring-shaped sliding clamp proteins are essential components of the replication machinery across all domains of life. DNA polymerases bind the cla

Excited to share another study from my lab @fordhamchem now out @narjournal.bsky.social‬: doi.org/10.1093/nar/.... Most bacterial DNA polymerases must interact with the sliding clamp DnaN for their function, but we show that it’s more complicated in B. subtilis DNA replication. (1/4)

Postdoctoral Fellow - Costa Lab Salary for this Role: From £45,500 with benefits, subject to skills and experience. Job Title: Postdoctoral Fellow - Costa Lab Reports to: Alessandro Costa Closing Date: 03/Aug/2025 23.59 GMT Job Desc...

Less than two weeks left to apply!

Postdoc positions in my lab to study

1. initiation of DNA replication.

2. chromatin replication/epigenetic inheritance.

Great for biochemists, biophysicists & structural biologists.

Deadline 3 August 2025.

crick.wd3.myworkdayjobs.com/External/job...

CDK-driven phosphorylation of TRAIP is essential for mitotic replisome disassembly and MiDAS Abstract. Disassembly of the replication machinery (replisome) from chromatin is an active process driven by two ubiquitin ligases Cul2LRR1 and TRAIP, whic

It is finally out 👍😀. Great job of the team (mostly absent from Bsky) and @m-saponaro.bsky.social

CDK-driven phosphorylation of TRAIP is essential for mitotic replisome disassembly and MiDAS url: academic.oup.com/nar/article/...

McGill University is seeking to recruit an internationally recognized, interdisciplinary scientist with a strong track record in innovation and research leadership to direct a new program in climate change, environment, and health that is supported by a prestigious CERC award. The CERC program supports Canadian universities in their efforts to build on Canada's growing reputation as a global leader in research and innovation. The program supports world-renowned researchers and their teams to establish ambitious research programs at Canadian universities. These awards are among the most prestigious and generous available globally. We seek an internationally recognized scholar whose research addresses the interconnected impacts of climate change, biodiversity loss, and/or ecosystem disruption on human health. The successful candidate will lead a bold, solutions-oriented research program that spans the life, environmental, and health sciences, with particular emphasis on bridging ecology, population health, and climate resilience through interdisciplinary tools and methods. The CERC Chairholder will be mandated to build a multidisciplinary research team that reflects a diversity of lived experiences, identities, and career stages. Their research may include emerging climate-health threats, biodiversity-related determinants of well-being and/or health, and interventions to reduce vulnerability and promote health equity in a changing environment. The Chairholder will also be expected to establish strong links with government agencies, civil society, and industry partners, as appropriate, to ensure equitable access to the knowledge produced and that social and health benefits that will flow from that knowledge.

Great opportunity for an established scientist to move and grow their research group in beautiful Montreal!

The Canada Excellence Research Chair (CERC) in Climate Change, Environment and Health at @mcgill.ca

Apply by Aug. 8.

Please share widely!

mcgill.wd3.myworkdayjobs.com/en-US/McGill...

Expanding the diversity of bacterial DNA partitioning: A CTP-independent ParABS system for plasmid partitioning in Streptomyces | PNAS The ATP- and CTP-dependent ParA-ParB-parS segrosome is a macromolecular complex that segregates chromosomes/plasmids in most bacterial species. CTP...

Finally published! Many thanks to the reviewers and editor whose comments & suggestions improved this work significantly more than the first preprint version!!!

www.pnas.org/doi/10.1073/...

Histone-mediated chromatin organization in prokaryotes and viruses Histones are fundamental chromatin-organizing proteins in eukaryotes and archaea, where they assemble into (hyper)nucleosomes that wrap DNA. Recent studies have expanded the known repertoire of histon...

A must-read for histone afficionados: our new review on 'Histone-mediated chromatin organization in prokaryotes and viruses' www.cell.com/trends/bioch... @samuelschwab.bsky.social @vikramalva.bsky.social

Congrats Nitin!

Spatiotemporal orchestration of mitosis by cyclin-dependent kinase - Nature Experiments in fission yeast show that cyclin-dependent kinase is first activated in the nucleus, from which the mitotic signal is propagated from CDK at the centrosome to the cytoplasm.

I'm very excited that my first postdoc paper is out now at @nature.com!
www.nature.com/articles/s41...

I developed new single cell CDK sensors to investigate how CDK orchestrates mitosis including investigating bistability/hysteresis, how activity is propagated, and the role of centrosomes.

Cell division protein CdpA organises and anchors the midcell ring in haloarchaea - Nature Communications Cell division in many archaea requires the coordinated activities of two distinct FtsZ proteins, which are part of the midcell division ring. Here, Liao et al. show that an additional protein, CdpA, o...

CdpA-an archaeal anchor for FtsZ! Excited to be part of this. (FtsZ is easily my favourite protein and the whole larger family in Archaea are intriguing).

www.nature.com/articles/s41...

FtsW protein-protein interactions visualized in live Staphylococcus aureus cells by FLIM-FRET The bacterial cell cycle relies on the coordinated and dynamic interactions between division proteins and those involved in peptidoglycan (PG) synthesis. However, visualizing these interactions in viv...

Check out this tour de force by Nils Meiresonne and colleagues, establishing FLIM-FRET as a reliable way to study protein interactions in live bacteria with spatiotemporal resolution! We open the door to exploring divisome protein interactions during cell division.

www.biorxiv.org/content/10.1...

Third track model for coordination of septal peptidoglycan synthesis and degradation by FtsN in Escherichia coli - Nature Microbiology Partitioning of FtsN and the septal peptidoglycan synthesis complex between an active synthesis track and an inactive denuded glycan track coordinates opposing peptidoglycan synthesis and degradation ...

Our new paper on FtsN is out! Now there are three tracks for organized cell wall constriction-- the old, boring E. coli always manages to surprise us.

A special shout-out to all the people who made this work possible, especially Dr. Jason Lyu, and Dr. David Weiss.

www.nature.com/articles/s41...

Single-molecule analysis reveals the mechanism of chromatin ubiquitylation by variant PRC1 complexes Single-molecule experiments show that active conformation formation controls chromatin ubiquitylation kinetics by variant PRC1.

Our new study of chromatin ubiquitylation by variant PRC1 on the single-molecule scale: We visualize directly how vPRC1 ubiquitylates neighboring nucleosomes during a single binding event, showing a potential mechanism how H2Aub domains are established.
www.science.org/doi/10.1126/...

A shared alarmone–GTP switch controls persister formation in bacteria - Nature Microbiology Alarmone synthesis depletes GTP levels leading to a GTP-dependent switch that controls triggered, spontaneous and antibiotic-induced persister formation in Bacillus subtilis.

www.nature.com/articles/s41...

Most human DNA replication initiation is dispersed throughout the genome with only a minority within previously identified initiation zones - Genome Biology Background The identification of sites of DNA replication initiation in mammalian cells has been challenging. Here, we present unbiased detection of replication initiation events in human cells using ...

New paper! Most human DNA replication initiation is dispersed throughout the genome with only a minority within previously identified initiation zones

genomebiology.biomedcentral.com/articles/10....

Identifying human DNA replication initiation sites on single molecules by @nanoporetech.com 1/7

I got hit by some rather sudden and extreme financial hardship so if anyone is in need of remote wetlab contract research, strictly BSL1, do let me know. Currently scrambling for gigs.

Plant, Bacterial, Archaeal Non-model Bioeng
Custom Lab Hardware
Turn Key Genetic Design

Please repost for reach 💚

Biofilm architecture determines the dissemination of conjugative plasmids | PNAS Plasmid conjugation is a contact-dependent horizontal gene transfer mechanism that significantly contributes to the dissemination of antibiotic res...

Biofilm architecture determines the dissemination of conjugative plasmids: We measured the spatiotemporal spread of plasmids in biofilms and found that plasmids only spread in some biofilm regions!

Collab with lab of @sbigot.bsky.social & Christian Lesterlin
www.pnas.org/doi/10.1073/...

A palette of bright and photostable monomeric fluorescent proteins for bacterial time-lapse imaging Bright and photostable monomeric fluorescent proteins enable long-term time-lapse imaging in E. coli.

Our set of new prokaryote-optimized fluorescent proteins is now published ! #microbiology #microsky #microscopy 🧫🦠🔬
www.science.org/doi/10.1126/...

Evolutionary Constraints on RNA Polymerase Gene Positioning in the Genome of Fast-Growing Bacteria.. The influence of gene order within the chromosome on cellular homeostasis and genome evolution remains unclear. Bacterial chromosomes are structured along the replication origin ( oriC )-terminus ( te...

And its out! Last work of the lab: "Evolutionary Constraints on RNA Polymerase Gene Positioning in the Genome of
Fast-Growing Bacteria."
We show how the genomic location of RNA polymerase impacts cell physiology. www.biorxiv.org/cgi/content/...

We are looking for a postdoc with microbiology background for #superresolution #singlemolecule #ERC tracking project. Apply by 27.4.2025. 🦠🔬🧫
Pls share!

www.aalto.fi/en/open-posi...

Molecular basis of ParA ATPase activation by the CTPase ParB during bacterial chromosome segregation DNA segregation by bacterial ParABS systems is mediated by transient tethering interactions between nucleoid-bound dimers of the ATPase ParA and centromere (parS)-associated complexes of the clamp-for...

Interested in the bacterial ParABS DNA segregation system? Then have a look at our latest preprint... 🧬

Thanks to the Hennig and Bange groups for the great collaboration!

doi.org/10.1101/2025...

Alt : Structure of the apo Lamassu protein complex. Schematic model of the active LmuA tetramer nuclease (top right). Lamassu icon (top left).

Glad to share the work of @yli18smc.bsky.social and co on Lamassu, a bacterial defense system related to Rad50/Mre11 (RM). While RM carefully trims DNA ends for repair, Lamassu chops up the host chromosome. Our study reveals how it is regulated to minimize damage, activating only during infection.

Excited to share my first postdoc paper - a tool to engineer mtDNA deletions in human cells and study their pathogenic impact ✂️🧬🧫

Rescuing bacterial genome replication: essential functions to repair a double-strand break and restart DNA synthesis Many antibiotics indirectly generate reactive oxygen species (ROS) that can damage bacterial genomes. Oxidised nucleobases become genotoxic when they are targeted for repair through excision, generati...

Ever wonder what happens when a bacterial replisome encounters a wee nick in the chromosome? Then check out this study by @winterhalterlab.bsky.social

What about a vertebrate replisome? Check out the work of @jamesmdewar.bsky.social

www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...

Side view of the structure of a monomer of type II Wadjet.

Happy to share the beautiful structure of a Wadjet SMC complex by @roisnehamelinf.bsky.social, with support from the DCI and @drhonsworth.bsky.social ! This is a type II Wadjet with unique characteristics including a tandem KITE subunit, distinct coiled coil architecture and a deviant hinge.