Steven Fanara

Broad application of plant protein tagging with ALFA tag for nanobody-based imaging and biochemical approaches Epitope tags are broadly used for detecting, modifying or purifying proteins of interest, but their breadth of application is often rather poor. Recently, the rationally designed ALFA tag and its ALFA...

Tired of struggling with different tags to generate transgenic plants expressing your favorite protein ? Check out our latest preprint about ALFA technology and its MANY applications for plant biology.
www.biorxiv.org/content/10.1...
@lrsv-toulouse.bsky.social @cnrsbiologie.bsky.social

20.03.2025 14:05 — 👍 44    🔁 30    💬 2    📌 0

I feel incredibly lucky to be surrounded by inspiring and driven colleagues and friends.

Science has never felt so fulfilling and balanced for me! 😀

16.02.2025 11:43 — 👍 2    🔁 0    💬 0    📌 0

My proposal was one of 1,966 submitted in the MSCA-LIF (Life Sciences) section this year. Despite my initial doubts, I am beyond thrilled to have been awarded this grant.

I am truly honored to have incredible supervisors who guide me in the right direction.

16.02.2025 11:42 — 👍 16    🔁 0    💬 2    📌 2

Quite a few new entries on the CRISPR list incl. @nevillesanjana.bsky.social @grunewald.bsky.social @sdomcke.bsky.social @rosswilsonlab.bsky.social @leopoldparts.bsky.social @bkleinstiver.bsky.social @mgdurrant.bsky.social

go.bsky.app/DEwe6ZD

19.11.2024 21:42 — 👍 22    🔁 12    💬 15    📌 0

Thank you very much! 😊

26.11.2024 16:47 — 👍 1    🔁 0    💬 0    📌 0

Hello! Thank you so much for this starter pack! Would it be possible to add me to it? I am working on RNA splicing (SR proteins) in Arabidopsis.

26.11.2024 14:04 — 👍 1    🔁 0    💬 1    📌 0

Thank you very much. 😊

26.11.2024 14:01 — 👍 1    🔁 0    💬 0    📌 0

Hi! Thank you for those starter packs! Can I please be added to the #1 and #4? 😊

25.11.2024 21:31 — 👍 1    🔁 0    💬 1    📌 0

🚨 Update for #RNA science fans:
I checked on #Bsky registry, and here is a 'condensate' (😆) of all #RNA #RNAsky starter packs so far:
1. General RNA biology #1
go.bsky.app/RN6HnZp

21.11.2024 08:08 — 👍 134    🔁 56    💬 29    📌 4

A profile picture of Douglas Prasher from Martin Chalfie's Nobel Lecture

Do you know who Douglas Prasher is? Many don't, even though he is the person who cloned the original #GFP gene in the late 1980s. In my short history of plant light #microscopy I also cover a bit of his story - & why he is relatively unknown today, despite the importance of his work. See this 🧵👇

20.05.2024 14:19 — 👍 285    🔁 149    💬 3    📌 24

When I performed SELEX assays with in vitro synthetized RNAs, I was using RiboSafe RNase Inhibitor from Bioline. I frequently checked the RNAs quality on gel and by measuring the A260/A230 ratio in quartz cuvettes. It never disappointed me.

For RNA-Seq samples, I used the RiboLock from Thermo.

17.11.2024 09:06 — 👍 2    🔁 1    💬 1    📌 0