klbr

At what resolution in cryo-EM do you see clear holes in aromatics? I see in some X-ray crystallography literature people are saying 1.6Å, but in cryo they seem quite visible at lower resolutions than this.

Employee directory lists them as "non-government," so they may not actually be "working for" NIH OD.

life achievement: replaced my chainsaw chain for the first time

The description of this system (and even many of the specific details) was terrifyingly familiar to me in NJ. I traipse through too many stories like this as a co-director of our local interfaith shelter. The enormity of the "wrongness" of it is difficult to sit with.

I live near Great Swamp but my first visit was underwhelming compared to nearby spots. Any recommendations of how to best experience it?

DOGE is going to part the Red Sea with AI

She couldn't resist the opportunity to explicitly say she's punishing Harvard for hiring former elected or appointed Democrats. This level of explicitly partisan score-settling is wrong and un-American.

I know I haven't been active on here much lately but...I am hiring a core facility postdoc to own the Krios, do a lot of cryoET jobs.rutgers.edu/postings/249...

this is in question

I know one Nobel laureate who wrote a novel that could fairly be described in this way but I think Narbonic was definitely funnier

I was thinking to myself "what were the best years so far for USA? maybe 1998 and 2014?"
This leads to my new hypothesis (nonserious) that the best years are going to be an inflection point in the median income (PPP-adjusted) with positive first derivative.

seems like you stole this from Twitter...used to be somebody's pinned in another time long long ago...

Trafficking studies that rely on following virus microinjected into the cytoplasm aren't valid if the virus in native cells would be inside a membranous organelle.

and yet New Jersey doesn't have a DMV: for the last 20+ years we've instead had a Motor Vehicles Commission (MVC)

exactly, so if the media is expired you will need to add more glutamine

well, it definitely will need glutamine supplementation

afaik Wolfson's chapter organized or participated in 3 protests against Trump's policies last month with 1 scheduled next week but not this Gaza action.

foiled!

maybe you wanted to drink from the chalice but it would take you so long to swallow that you have to intinct now!

We just got to the point where we couldn't do the experiments we absolutely needed to do without on-site 300kV, so we added a Krios on top of our Tundra & Arctica. If we'd had the money 8 years ago, we would've just started with the Krios, but things unfold in circuitous paths.

It's actually a big issue...service contracts are so expensive that if we can't use enough of the 'scope time, I may have to cut staff.

Now is the time for solidarity between people (nominally of all parties) who believe NIH should be significantly reworked or cut and people who believe we should do the same but moreso, since the enemy of both is radicals who believe in smashing everything for the sake of revenge/malice/incompetence

I think that many reasonable & knowledgeable people disagree on that count, but the whole point of the article is that people who /do/ think ICR should be reformed ought to refrain from giving ammo to their own enemies since the admin. is not making good-faith efforts at reform.

Since I got a third cryo-transmission electron microscope, I have more 'scope time than I know what to do with. If you have a project that requires a large amount of data collection, get in contact because we can probably work something out.

This is also a huge part of being a facility director, but instead of 100 charging cables it is 100 miscellaneous communications cables with ends you can't quite identify as any commercially-available connector.

As one of the ESI about to be affected (MRAE for me, so I don't think it's a conflict of interest for me to be commenting on your post...), thank you for expressing your solidarity. It is some comfort to know that more senior investigators see the special impact on people trying to launch programs.

Yuval Levin: The deeper question raised by the NIH grant overhaul Is the Trump administration’s goal to run the government or oppose it?

Conservative writer Yuval Levin agrees with you and I think his essay on this is well-crafted and worth a read: www.thenewatlantis.com/publications...

"Scientific facts don't care about your feelings"

Don’t be a useful idiot puppet for the regime I say this to myself regularly in the past weeks, mostly when it comes to sitting on my hands about my regular NIH-related and academic science career-related hobby horse issues as they arise. We h…

Don't be a useful idiot puppet for the regime drugmonkey.wordpress.com/2025/02/25/d...

For someone outside the field, your post is an exciting and clear. It's really cool to see the accumulation of evidence shape our understanding of these events.