Giorgia Tosoni

Activation of transposable elements is linked to a region- and cell-type-specific interferon response in Parkinson's disease Parkinson's disease (PD) is a common age-related neurodegenerative disorder involving a neuroinflammatory response, the cause of which remains unclear. Transposable elements (TE) have been linked to i...

New preprint from my lab! We describe how transposable elements are activated in Parkinson’s disease, which is linked to an interferon response. We believe this study significantly advances our understanding of transposons and their role in human brains.

www.biorxiv.org/content/10.1...

LINE-1 retrotransposons mediate cis-acting transcriptional control in human pluripotent stem cells and regulate early brain development Adami et al. demonstrate that evolutionarily young L1s are expressed in human pluripotent stem cells and are dynamically regulated throughout neural differentiation. The study examines the role of L1s...

New paper from our lab! We found that LINE-1 transposons contribute to early human brain development.

Funded by @asapresearch.parkinsonsroadmap.org

www.cell.com/cell-genomic...

An integrated single-nucleus and spatial transcriptomics atlas reveals the molecular landscape of the human hippocampus - Nature Neuroscience The topographical organization of cells in the hippocampus reflects its ability to regulate mood and cognition. Here the authors generate a spatially resolved gene expression map in the human hippocam...

Excited to share this huge team effort collaboration supervised by Stephanie Page in Translational Neuroscience @lieberinstitute.bsky.social and @stephaniehicks.bsky.social @jhubiostat.bsky.social to generate a spatio-molecular resource of the hippocampus in human 🧠

www.nature.com/articles/s41...

The elderly brain is less mature than you think and that could protect you from Alzheimer’s Disease - Netherlands Institute for Neuroscience - Master the Mind

Why do some people with Alzheimer’s do not show symptoms of Alzheimer's?

During her PhD, @giorgitos.bsky.social explored this question. Part of the answer led her to an unexpected group of brain cells: underdeveloped neurons.

Read the full article on our website: nin.nl/news/the-eld...

Important perspective on a fascinating study from the Frisén group, which used machine learning to identify neural progenitors in the adult human brain, a fresh lens on a long-standing debate 💥
Always exciting to see this field evolve, and grateful to be part of it @labsalta.bsky.social

Hora est 🎓
Last Thursday, I defended my PhD at the University of Amsterdam, @nin-knaw.bsky.social—graduated cum laude ✨ Huge thanks to my amazing supervisor @labsalta.bsky.social, co-promoters Paul Lucassen, Inge Huitinga & @fitzsimonslab.bsky.social. Thanks to all who made the day unforgettable!

I'm proud of contributing to @nkrauth.bsky.social @clemmensenc.bsky.social @olekiehn.bsky.social discovery of the role of an hypothalamic-brainstem circuit for prioritization of safety. Really impressive amount of work led by @nkrauth.bsky.social

Unique transcriptional profiles of adult human immature neurons in healthy aging, Alzheimer’s disease, and cognitive resilience The existence and functional significance of immature neurons in the adult human brain, particularly in the context of neurodegenerative disorders, remain controversial. While rodent studies have high...

@labsalta.bsky.social identified persistent populations of immature neurons that were associated with ‘juvenile’ cellular functions, suggesting that these immature populations may actively contribute to maintaining homeostasis within the aged human hippocampus.
www.biorxiv.org/content/10.1...

Our paper has sparked a lot of people asking me – so Keri, what’s the tldr – is there, or isn’t there, new neurons in the human hippocampus?

It's a little more complicated than a yes or no IMO.

Excited to share our preprint led by Ain Chung
& @jasonalipio.bsky.social showing how immature adult-born DGCs preferentially recruit inhibition of CA2 and CA3 to disproportionately affect hippocampal network properties and social memory. We began by asking 1/n
www.biorxiv.org/content/10.1...

Graphical abstract from the paper depicting the overall study design

Updated from the preprint, our paper, "Spatiotemporal analysis of gene expression in the human dentate gyrus reveals age-associated changes in cellular maturation and neuroinflammation" is now published.

www.cell.com/cell-reports...

very interesting!!

Comparative molecular landscapes of immature neurons in the mammalian dentate gyrus across species reveal special features in humans Immature dentate granule cells (imGCs) arising from adult hippocampal neurogenesis contribute to plasticity, learning and memory, but their evolutionary changes across species and specialized features...

Check out our latest preprint, where we cross-compared the molecular landscapes of immature neurons in the hippocampus in different mammalian species👨🐵🐷🐭, revealing specialized features in humans. www.biorxiv.org/content/10.1...

I've been developing a semantic search tool that covers not just bioRxiv and medRxiv, but the entire PubMed database. This means you can search across a massive collection of biomedical research using keywords, questions, hypotheses, or even full abstracts. Try it out: mssearch.xyz

The NINclusion team launched the NIN Gym to support mental and physical health within the institute. Over the next 10 weeks, employees can join alternating Yoga and Bootcamp sessions led by personal trainer Ties
(@tiesverbeekpt.bsky.social) on Thursdays. The first session took place last week! 💪

All NIH study sections canceled indefinitely. This will halt science and devastate research budgets in universities.

@labsalta.bsky.social @nin-knaw.bsky.social @codingdilara.bsky.social

12/ Huge thanks to all the co-authors, brain donors, and their families—this work wouldn’t have been possible without you! 🙏 We would be super grateful to hear your thoughts on our findings (don’t hold back, we’re all ears)! #Neurogenesis #Alzheimers #resilience

11/ Our work suggests immature neurons actively support hippocampal plasticity and resilience. Could targeting these cells help unlock new strategies for combating AD? 🏥💡We hope to provide a resource to help explore the molecular basis of aging, AD, and resilience. 🛠️

10/ ImN also showed decreased (in silico) intercellular communication in AD brains. In resilient individuals, they maintained crosstalk with other cells critical for anti-inflammatory and neuroprotective signaling. 🤝

9/ 🛡️ Resilient individuals—cognitively intact despite AD pathology—harbored ImN enriched for anti-inflammatory, anti-amyloidogenic, and neuroprotective transcriptional programs. These cells may actively buffer against AD progression. 💡

8/ We took extra steps to validate our findings using RNAScope in adult and fetal human brain & human iPSC-derived 3D cultures. Key marker combinations could detect ImN, and increased with hippocampal neuronal differentiation, indicating a role in human hippocampal neurogenesis. 🔬

7/ Yet, are these cells born in adulthood, passively waiting to mature when they ‘grow up’ or lingering remnants of embryonic neurogenesis? Maybe neither: ImN showed “youthful” transcriptional traits vs mature neurons, suggesting they may actively support hippocampal homeostasis.

6/ So, what did we find? Our quality control pipeline revealed the presence of immature neurons in adult GC! 🥳Enriched for immature markers, these populations were validated by trajectory inference and integration with the fetal neurogenic dataset.

5/ Back to the adult cohort: To screen for ImN signatures in adult GC and avoid false positives & negatives, we had to apply a rigorous pipeline: Ambient RNA removal, astrocyte and GC subsetting, empty droplet artifact filtering, unspliced RNA re-clustering. 🧹

4/ But what’s a good positive control for something that has not yet been thoroughly characterized? 🤔
We used fetal hippocampal tissue as a reference. In the fetal dentate gyrus, we observed clear neurogenic trajectories: neural stem cells → progenitors → ImN.

3/ We had to enrich: We developed a new isolation protocol for sampling the dentate gyrus (granular cell layer & subgranular zone). This boosted granule cell (GC) detection to 54% (vs. 7–23% in prior studies) and enabled deep profiling of rare ImN signatures using snRNA-seq. 🎯

2/ What’s the deal with adult human ImN? These rare cells are known for being harder to find than a needle in a haystack. We profiled 110,527 high-quality nuclei from the hippocampus of 24 (healthy, moderate & severe AD, and dementia-resilient) donors using snRNA-seq.

🧵 1/ Excited to share our new research on immature neurons (ImN) in the adult human brain and their roles in healthy aging, Alzheimer’s disease (AD), and cognitive resilience. Here’s what we discovered 👇

Big achievement for the green office (and not only!), finally LEAF is implemented in our Institute @nin-knaw.bsky.social 💚