Naomichi Takemata

Congrats!!!

Lab’s first paper is out!! We show the first structures of #Asgard #chromatin by #cryo-EM 🧬❄️
Asgard histones form closed and open hypernucleosomes. Closed are conserved across #Archaea, while open resemble eukaryotic H3–H4 octasomes and are Asgard-specific. More here: www.cell.com/molecular-ce...

This was a fun collaboration between Ophélie & the @seegerlab.bsky.social using sybody libraries to target the SMC complex in living bacteria. With a suprising finding: the 14 isolated sybodies bind to the middle of the SMC coiled coil rather than the more conserved ATPase heads.

Check out our paper on intricate nested interactions between viruses and virus satellites of haloarchaea and their nanosized DPANN symbionts. Excellent collaboration with @deemteam.bsky.social, @anagtz.bsky.social and Michail Yakimov
Free access link: rdcu.be/eLtCH
🧵 by @yifanzhou.bsky.social 👇

Temporal and spatial coordination of DNA segregation and cell division in an archaeon | PNAS Cells must coordinate DNA segregation with cytokinesis to ensure that each daughter cell inherits a complete genome. Here, we explore how DNA segre...

Our paper on chromosome segregation in the archaeon Sulfolobus Acidocaldarius has been published in PNAS! This has been a great collaboration with @joeparham19.bsky.social and the rest of @buzzbaum.bsky.social's team. Stay tuned for more to come!😉 @istaresearch.bsky.social

doi.org/10.1073/pnas...

Happy that the final version of our Lamassu work @yli18smc.bsky.social is now out:

www.nature.com/articles/s41...

Thanks again to our awesome collaborators @mblokesch.bsky.social and David and co and Mark Szczelkun and @steven-shaw.bsky.social and the DCI Lausanne @fbm-unil.bsky.social

Versatile NTP recognition and domain fusions expand the functional repertoire of the ParB-CTPase fold beyond chromosome segregation Nucleotide triphosphate (NTP)-dependent molecular switches regulate essential cellular processes by cycling between active and inactive states through nucleotide binding and hydrolysis. These mechanis...

new preprint from our group & Antoine Hocher: www.biorxiv.org/content/10.1...
A fantastic collaboration with Antoine, with Jovana Kaljevic' initiated the collaboration and drives the project.

How do SMC complexes load onto DNA to get ready for loop extrusion?

@roisnehamelinf.bsky.social & co discovered that Wadjet, an SMC complex involved in bacterial DNA immunity, performs some impressive molecular gymnastics 🤸‍♂️🤸‍♂️🤸‍♂️.

Check out the new paper: www.cell.com/molecular-ce...

Dynamics and interplay of gene expression and chromosome organization across a predatory lifecycle The obligate predatory bacterium Bdellovibrio bacteriovorus alternates between a motile attack phase and a growth phase inside another bacterium, during which it undergoes multiple rounds of DNA repli...

📝New preprint! #MicroSky

🕰️We chart the full, synchronized lifecycle of a predatory bacterium using Hi-C, RNA-seq, ChIP-seq & microscopy.

🧬We uncover dramatic shifts in nucleoid architecture & transcription, tightly coordinated with predation.

www.biorxiv.org/content/10.1...

Thank you to @tamarabasta.bsky.social for hosting my visit to I2BC @i2bcparissaclay.bsky.social in Paris! I truly enjoyed the inspiring scientific interactions and the beauty of the city.

Our Wadjet-II paper by @roisnehamelinf.bsky.social is out in polished form: www.cell.com/structure/fu...

With a great preview by @kevincorbett.bsky.social & @amardeeep.bsky.social highlighting the potential of Wadjets:

Wadjet—Keeping a watchful eye on circular DNA.

www.cell.com/structure/fu...

Our new preprint is out!
We combined single-nucleosome imaging and 3D-SIM to reveal:
🔹 Euchromatin forms condensed domains, not open fibers
🔹Cohesin loss increases nucleosome mobility without decompaction
🔹Cohesin prevents neighboring domain mixing

Full story & movies👇

Check out our paper in PLoS Genetics with @umbertorosani.bsky.social and Marco Gerdol on the highly dynamic pangenome of basal chordates. It is enriched in defence and immunity genes (like in prokaryotes) and follows Mendelian inheritanceis rules. @plos.org journals.plos.org/plosgenetics...

Archaea produce peptidoglycan hydrolases that kill bacteria Archaea regularly interact with bacteria but reports of archaea killing bacteria are very rare. This study shows that many archaea encode peptidoglycan hydrolases, which specifically target bacterial ...

Archaea are often surrounded by bacteria. But is there ever active conflict between the two? Can archaea kill bacteria? If so, how do they do it?

Work by @romainstrock.bsky.social shows that some archaea can kill bacteria by secreting peptidoglycan hydrolases. journals.plos.org/plosbiology/...

My attempt to become a professor was unsuccessful again but the diplonema tagging paper is now published in Open Biology. royalsocietypublishing.org/doi/10.1098/...

📣Huge congrats to our postdoc, Takaya Totsuka, on his new paper @currentbiology.bsky.social 🎉 He found that Ca2+ oscillations regulate oocyte spindle rotation! 🐭The project started during his PhD with Prof. Miho Ohsugi.

Free link: authors.elsevier.com/c/1lUqk3QW8S...

Strategies and mechanisms of contact-dependent predation in bacteria Predatory bacteria kill other bacteria to acquire nutrients. Among diverse strategies to kill and feed on prey, contact-dependent predation stands out…

#MicroSky Summer read!

Predatory bacteria kill and eat other bacteria. Their deadly actions come in many flavors, often via direct physical contact between predator &
prey 🧛

How do they do it?
Our review 📖 @coralietesseur.bsky.social @ysantin.bsky.social

www.sciencedirect.com/science/arti...

Congrats!!!

And the story is published now!🍾
www.nature.com/articles/s41...

Open access link: rdcu.be/exnOc

Many years in the making, great collaboration with @archaellum.bsky.social & @tunglejic.bsky.social

Thanks @ukri.org BBSRC & @leverhulme.ac.uk for funding, reviewers & editor!! 🙏
#microsky #archaeasky

🚨Our latest work on selfish centromeres is published @currentbiology.bsky.social 🐭🧬We found that the spindle checkpoint contributes to non-Mendelian segregation! Huge congrats to our lab manager, Zaak Walton, who led the project🎉

Free link:
authors.elsevier.com/a/1lQzN3QW8S...

Our new preprint 🧬🔗 www.biorxiv.org/content/10.1...
BRD4-NUT forms liquid-like condensates that locally constrain nucleosomes via BRD4-mediated crosslinking— physical control of #chromatin by #LLPS transcription condensates. @semeigazin.bsky.social @katsuminami.bsky.social @masaashimazoe.bsky.social

We found a new asymmetry in the large-scale chromosome structure: sister chromatids are systematically shifted by hundreds of kb in the 5′→3′ direction of their inherited strands! The work was led by Flavia Corsi, in close collaboration with the Daniel Gerlich lab.
www.biorxiv.org/content/10.1...
1/

The role of nucleoid-associated proteins in mediating responses to environmental changes Bacteria face diverse environmental challenges, such as changes in temperature, pH, and osmolarity, and exposure to antibiotics, which necessitate ada…

I am pleased to share our new review on the role of bacterial nucleoid-aasociated proteins in mediating responses to changes in environment. Important: some NAPs can directly ‘sense’ physico-chemical changes. www.sciencedirect.com/science/arti... @pingzhuangge.bsky.social

Fantastic opportunity to start your independent career at the JIC here. Great startup package. Repost = nice. Thank you!!!

Delighted to share a new review paper from the lab. We summarized/discussed recent key findings in enhancer biology. Thanks Dan Larson and @tinekelenstra.bsky.social for this opportunity!!

www.sciencedirect.com/journal/jour...

New preprint from Kelsey Fryer in the lab using ChAR-seq to identify RNAs that are bound to human centromeres. Lots of surprises! #centromere #RNA www.biorxiv.org/content/10.1...

Our new review paper is out@JMB!🧬
@katsuminami.bsky.social‬ @semeigazin.bsky.social‬ ‪@kakonakazato.bsky.social‬ discuss DNA accessibility in euchromatin and heterochromatin of live cells.
Free access: authors.elsevier.com/a/1lHgb54HFZ...
Also check our previous work: www.science.org/doi/10.1126/...

This reveals parallels with DNA end protection in eukaryotes and highlights the challenges of having linear chromosomes as well as functional repair of DNA double strand break.

www.biorxiv.org/cgi/content/...

Published now. doi.org/10.1242/jcs....

JAMSTEC Young Research Fellow (JYRF) | JAMSTEC - JAPAN AGENCY FOR MARINE-EARTH SCIENCE AND TECHNOLOGY JAMSTEC Young Research Fellow (JYRF)

Would you like to work with us?
At JAMSTEC, we are recruiting postdoctoral researchers through the Young Research Fellow program.
This program allows fellows to independently pursue their own research projects.
www.jamstec.go.jp/recruit/e/jy...