Brian Kleiboeker

and F30! www.grants.gov/search-resul...

Direct sensing of dietary ω-6 linoleic acid through FABP5-mTORC1 signaling Diet influences macronutrient availability to cells, and although mechanisms of sensing dietary glucose and amino acids are well characterized, less is known about sensing lipids. We defined a nutrien...

Direct sensing of dietary ω-6 linoleic acid through FABP5-mTORC1 signaling | Science www.science.org/doi/10.1126/...

It’s hard to focus lately and feels wrong to talk about science when the whole system is under threat, but I’ve been reading Hans Krebs’ autobiography and it’s excellent. I highly recommend for all scientists, especially biochemists

Looks like Emory is in the first listed association? Hopefully the suit is filed on behalf of all schools in the associations too bc Pitt is in the same boat

FSP1-mediated lipid droplet quality control prevents neutral lipid peroxidation and ferroptosis Lipid droplets (LDs) are organelles that store and supply lipids based on cellular needs. While mechanisms preventing oxidative damage to membrane phospholipids are established, the vulnerability of L...

Excited to share our new preprint! Exceptional work by @mikelangelipid.bsky.social identifies the first #LipidDroplet lipid quality control pathway! LD-localized FSP1 safeguards neutral lipids from peroxidation and prevents #ferroptosis. 🤩https://www.biorxiv.org/content/10.1101/2025.01.06.631537v1

Note that the "Fas" in FAF2 is the apoptosis Fas, not the fatty acid synthase Fas. Confusing, given the context!

CRISPR screening in epithelial cells suggests that peroxisome-derived ether lipids alter cellular viability in response to lipotoxic stress. Fas-associated factor family member 2 (FAF2) appears to be a key regulator of this process. This is an interesting preprint!

www.biorxiv.org/content/bior...

Aspirin modulates generation of procoagulant phospholipids in cardiovascular disease, by regulating LPCAT3 Enzymatically oxygenated phospholipids (eoxPL) from lipoxygenases (LOX) or cyclooxygenase (COX) are pro-thrombotic. Their generation in arterial disease, and their modulation by cardiovascular therapies is unknown. Furthermore, the Lands cycle acyl-transferases that catalyze their formation are unidentified.

To my reading, this paper suggests a role for 'classic' #ferroptosis mechanisms (namely LPCAT3 and oxPL) in ASCVD in humans

www.jlr.org/article/S002...

I learned so much from this episode when I listened a few months ago!

Ok, trying to bring my community back together... I created a starter pack for lipids and membrane protein aficionados!

Feel free to share, add and distribute, so we can come together again! 😍

go.bsky.app/AEeXh86

I have initiated this #StarterPack with #RedoxBio researchers.

Feel free to suggest other users, or yourself, to be included!

go.bsky.app/UoVRtXp

Congratulations and welcome to Pitt!

Waiting for the AI people to come say that this take just means you don’t know how to engineer prompts correctly 😂

#StartPack for the #Ferroptosis researchers community.

A small but growing list—let’s expand it together!

Please, feel free to reach out if you'd like to be added or removed. 🧪🔬

go.bsky.app/35UvtTi

tWiptophan is hilarious

🎨 In case anyone need…

The NIH BioArt Source is an awesome library of *free* professionally drawn illustrations for scientific presentations or figures. Downloadable in HD. Thank you NIH for this invaluable tool 🙏!

Check it out 👇
bioart.niaid.nih.gov

mTORC1 activity licenses its own release from the lysosomal surface The activation of mTORC1 is intricately linked to its subcellular localization, with the Rag GTPases controlling its lysosomal recruitment in response to nutrient cues. Acharya and Demetriades uncover...

mTORC1 activity licenses its own release from the lysosomal surface
www.cell.com/molecular-ce...

Will be interesting to see if it is as versatile as ggplot2! I’ve yet to find something I can’t do in ggplot, though sometimes it does take…a lot of lines of code

NLRP1 is activated by palmitic acid and induced in human metabolic dysfunction-associated steatohepatitis https://www.biorxiv.org/content/10.1101/2024.11.11.622769v1 Metabolic dysfunction-associated steatotic liver disease (MASLD) is the most prevalent cause of live

NLRP1 is activated by palmitic acid and induced in human metabolic dysfunction-associated steatohepatitis https://www.biorxiv.org/content/10.1101/2024.11.11.622769v1

I think that there is a lot more to learn at this interesting nexus of phospholipid and triglyceride metabolism in adipose tissue!

Adipose tissue peroxisomal lipid synthesis orchestrates obesity and insulin resistance through LXR-dependent lipogenesis - PubMed These studies reveal an unexpected role for peroxisome-derived lipids in regulating LXR-dependent lipogenesis and suggest that activation of lipogenesis, combined with dietary lipid overload, exacerba...

I helped with a related story in Irfan Lodhi's lab at WUSTL. We found a link between peroxisome-derived phospholipids and lipogenesis (involving LPCAT3) with effects on systemic metabolism.

pubmed.ncbi.nlm.nih.gov/38458567/

Dietary control of peripheral adipose storage capacity through membrane lipid remodelling Complex genetic and dietary cues contribute to the development of obesity, but how these are integrated on a molecular level is incompletely understood. Here, we show that PPARγ supports hypertrophic ...

This is a really interesting new preprint by Peter Tontonoz's group at UCLA. They discover a PPARγ–LPCAT3 pathway that links dietary n–6 PUFA intake to adipose expandability and systemic energy balance through unique storage of triglycerides.

www.biorxiv.org/content/10.1...

The PNPLA3 I148M variant increases ketogenesis and decreases hepatic de novo lipogenesis and mitocho... The PNPLA3 I148M variant is the major genetic risk factor for all stages of fatty liver disease, but the underlying pathophysiology remains unclear. W…

This was a good read - some very exciting translational science!

"The PNPLA3 I148M variant increases ketogenesis and decreases hepatic de novo lipogenesis and mitochondrial function in humans"

... a #lipidomic analysis of P. gingivalis and report several previously unidentified lipid families. Read the full, open-access story in JLR!

#massspec #massspectrometry #teammassspec

Lipidomic analysis of Porphyromonas gingivalis reveals novel glycerol bisphosphoceramide, phosphatid... Porphyromonas gingivalis, like other members of the phylum Bacteroidetes (synonym Bacteroidota), synthesizes several classes of dihydroceramides and p…

Porphyromonas gingivalis is a gram-negative anaerobic bacterium which is commonly found in the oral cavity and has been implicated in the pathogenesis of chronic periodontitis, but is also associated with other conditions such as bacterial vaginosis and Alzheimer’s disease. Here, we perform ...

I imagine that this is a ‘lower limit’ of sorts and that variability would only increase in real life scenarios where scientists are biased by their own hypotheses and trying to use the data in a publication!

This recent paper shows a role of MMD in forming a metabolon-like complex that serves to incorporate AA into PI phospholipids, thereby promoting ferroptosis. Very exciting stuff!

This summer I worked on a rotation project looking at the role of arachidonic acid (AA) remodeling into macrophage phospholipids in atherosclerosis, a topic about which there’s some interesting literature dating back to 1994 (PMID: 8181456)....

I’ve known ppl who couldn’t stay consistent lifting but fell in love with pickup basketball and now play 5days/wk. People who hated going to the gym but got a peloton at home and love the private yet structured format. And ofc the myriad irl classes takes time to explore but suits a lot of ppl also

Honestly, I think it’s just because I’ve found a type of exercise that I really enjoy. I go to the gym every day because I want to, not because I have to. imo, finding a type of exercise that’s enjoyable is the biggest factor for consistency, bc pure motivation/commitment an only take you so far.