Sdelci Lab

Big congrats to Sara Sdelci @sdelcilab.bsky.social for being as one of the 2025 @embo.org Young Investigators (YIPs), joining 28 outstanding early-career scientists recognised for excellence and originality in the life sciences. She is the eighth CRG scientist to join the prestigious programme!

I am (Sara Sdelci, @crg.eu) very happy to have been selected as an @embo.org YIP! Thank you for selecting our project on how nuclear metabolism shapes cellular proliferation and aging; we are truly excited for the beautiful science ahead!

We did it in cancer cell lines and validated the phenotype in cancer tissues

Not IN, they are compressing the nucleus from the outside ;)

Mitochondria-derived nuclear ATP surge protects against confinement-induced proliferation defects - Nature Communications The authors uncover a mechano-metabolic adaptation where confinement induces rapid mitochondrial relocalization to the nuclear periphery, generating localized nuclear ATP surges that support chromatin remodeling, DNA repair, and cell cycle progression.

Earlier this summer we have published this. Looks like a similar localization of mitochondria in the centre of the nucleus: www.nature.com/articles/s41...

Dr. Jane Goodall has passed away. Her groundbreaking work with chimpanzees and her lifelong dedication to conservation changed how we see and care for our planet.

Happy to share the Biodiversity Cell Atlas white paper, out today in @nature.com. We look at the possibilities, challenges, and potential impacts of molecularly mapping cells across the tree of life.
www.nature.com/articles/s41...

Science: only when you write up the manuscript, you realize what you should have done.

Being open to new ideas: Metabolic Enzymes in the Nucleus as Chromatin Instructors?

Merging
#chromatin
#epigenetics
#metabolism
To understand new cellular rules.

Uncovering how mechanical forces can influence nuclear ATP levels, with downstream effects on DNA damage response and cell cycle progression.

#Mechanobiology #NuclearMetabolism #CellFate #DNArepair #CellCycle

Mitochondria-derived nuclear ATP surge protects against confinement-induced proliferation defects - Nature Communications The authors uncover a mechano-metabolic adaptation where confinement induces rapid mitochondrial relocalization to the nuclear periphery, generating localized nuclear ATP surges that support chromatin...

🚀 We’re excited to share our latest publication in collaboration with Verena Ruprecht's group at @crg.eu

Mechanobiology meets Nuclear Metabolism

www.nature.com/articles/s41...

Fantastic to see this wonderful piece out! Happy to have contributed to this effort 💪

🔬 ¡Buscamos Técnico/a de laboratorio!
📋 Mantenimiento de colonia, procesamiento de muestras y más.
📍 Barcelona
📩+info: hospitalsantjoandedeu.talentclue.com/es/node/1129...

Please RT!

BIG THANKS to everyone involved, especially Toni for his amazing work!

Thanks to our sponsors:
@ageinves.bsky.social
@ERC_Research #ERCStG EPICAMENTE
@crg.eu

Open questions:
Is nuclear metabolism linked to #aging?
Can we reverse the aging process by restoring nuclear metabolism?
What else besides PIP2?

If you are interested in answering these questions, please contact us!

Our study links nuclear PIP2 metabolism to nucleoli structure, histone modification, and cell cycle progression. This provides evidence that nuclear metabolism oscillations can control cell cycle progression, which, when altered, can induce cell cycle arrest and cell senescence.

4) The aberrant localization of PIP2 in the nucleus/nucleoli and the alteration of the nucleoli structure lead to changes in the accumulation of histone marks. For example, H4K20me1 decreases, which subsequently affects proper cell cycle progression.

3) What happens to cells when we interfere with PIP2 metabolism in the nucleus? In addition to preventing cell cycle progression, the structure of the nucleolus is affected and the localization of PIP2 to the Fibrillarin-labeled nucleoli region is altered.

2) We used our lab's gold standard chromatome protocol to study the oscillation of metabolic enzymes on chromatin during the cell cycle. We identified a correlation between cell cycle progression and PIP2 metabolism on chromatin.

1) By integrating FUCCI reporters and classical proliferation/senescent markers, we identified a population of cells, which we termed G0-senescent, that had never before been identified using FUCCI.

Our initial question was: does the cell cycle control the association of metabolic enzymes with chromatin? The short answer is YES!
We adapted the FUCCI system and extensively characterized U2OS FUCCI using HT-microscopy and FACS.

As promised, here is our NEW preprint on CELL CYCLE ASSOCIATED PIP2 NUCLEAR METABOLISM by our SUPER postdoc Toni Gañez, who knows all the secrets of FUCCI reporters 👇
www.biorxiv.org/content/10.1...

Open questions:
Is nuclear metabolism linked to aging?
Can we reverse the aging process by restoring nuclear metabolism?
What else besides PIP2?

If you are interested in answering these questions, please contact us!

Our study links nuclear PIP2 metabolism to nucleoli structure, histone modification, and cell cycle progression. This provides evidence that nuclear metabolism oscillations can control cell cycle progression, which, when altered, can induce cell cycle arrest and cell senescence.

4) The aberrant localization of PIP2 in the nucleus/nucleoli and the alteration of the nucleoli structure lead to changes in the accumulation of histone marks. For example, H4K20me1 decreases, which subsequently affects proper cell cycle progression.

3) What happens to cells when we interfere with PIP2 metabolism in the nucleus? In addition to preventing cell cycle progression, the structure of the nucleolus is affected and the localization of PIP2 to the Fibrillarin-labeled nucleoli region is altered.

2) We used our lab's gold standard chromatome protocol to study the oscillation of metabolic enzymes on chromatin during the cell cycle. We identified a correlation between cell cycle progression and PIP2 metabolism on chromatin.

We adapted the FUCCI system and extensively characterized U2OS FUCCI using microscopy and FACS.
1) By integrating FUCCI reporters and classical proliferation/senescent markers, we identified a population of cells, which we termed G0-senescent, that had never before been identified using FUCCI.

Our initial question was: does the cell cycle control the association of metabolic enzymes with chromatin? The short answer is YES!

Merry Christmas from the Sdelci Lab!

Stay tuned for our last preprint before the end of the year... lots of FUCCI colors 🎄-style and metabolic enzymes on chromatin during cell cycle progression
COMING SOON 💥