Wilfried Rossoll

We’re hiring! Two postdoc positions are open in the Translational Neuroproteomics lab at Mayo Clinic Florida, focusing on tau and TDP-43 proteinopathies and neurodegeneration in ALS, FTD, and AD.
www.mayo.edu/research/lab...
Apply here:
1) tinyurl.com/2p9n59y5
2) tinyurl.com/em2zpu2j
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Direct interaction between TDP-43 and Tau promotes their co-condensation, while suppressing Tau fibril formation and seeding
@dormannlab.bsky.social
www.embopress.org/doi/full/10....

Engineering antisense oligonucleotides for targeted mRNA degradation through lysosomal trafficking Antisense oligonucleotides (ASOs) can modulate gene expression at the mRNA level, providing the ability to tackle conventionally undruggable targets and usher in an era of personalized medicine. A key...

We have developed an entirely new mechanism of action for gene knockdown -> trafficking of targeted mRNAs to the lysosomal, using lysosomal trafficking oligonucleotides (LyTONs)!

Published in @chemicalscience.rsc.org
Led by Disha Kashyap
#chemsky 🧪
pubs.rsc.org/en/content/a...

I heard that they are working on an adapter to defeat the pressure sensor present in the Flex (but not the OT-2) that prevents it from working with the tips…..

Opentrons was supposed to present their adapter to make it work on the Flex at ASMS. They say it is delayed and beta-testing is only for their 96-channel pipette now. They say they are still working on a solution for the 8-channel Pipette.

We are thrilled that Prof Philip Wong, Ph.D., of John Hopkins Medicine will be our next webinar speaker!

"An AAV gene therapy for #ALS-#FTD guided by a presymptomatic #biomarker"

May 29, 2025
1:30-2:30 PM (ET)
Host: @rossolllab.bsky.social
Register: bit.ly/4jRnCDa

Context-dependent Interactors Regulate TDP-43 Dysfunction in ALS/FTLD

TDP-43 APEX proximity labeling to identify interactors for cytoplasmic (ΔNLS) and RNA-binding deficient (5FL) TDP variants!

By Longxin Xie and @donnellylab.bsky.social.

www.biorxiv.org/content/10.1...

I was thrilled for the opportunity to present today #ADPD2025 the work @PrudencioLab, in the wonderful city of Vienna where I was lucky to get an awesome tour by Dr Wilfried Rossoll! @rossolllab.bsky.social

Research Fellow: Neuroscience - DEMENTIA RESEARCHER Join Dr Grinberg’s team at Mayo Clinic exploring sleep dysfunction in neurodegenerative diseases, with advanced training and exceptional career prospects.

Join Dr Grinberg’s team at Mayo Clinic exploring sleep dysfunction in neurodegenerative diseases, with advanced training and exceptional career prospects. Closing date: 25th May

www.dementiaresearcher.nihr.ac.uk/job/research...

Thank you!

Thank you!

Thank you!

Thank you!

Our study provides a first comprehensive overview of pTau-associated proteomes across tauopathies and uncovered several important findings. It is also a starting point for further ongoing ProPPr projects, where will put everything that we have learned during this long journey to good use.

Our further analysis demonstrated that infiltration of astrocytic plaques with FTL-positive microglia is a characteristic feature of CBD pathology. The observed complex co-localization patterns underscore the importance of using fluorescence microscopy to follow up on proximity proteomics findings.

To discover proteins with differential association with pTau lesions between AD, CBD, PiD and PSP, we performed statistical comparison of protein abundances with MiST. We found a strong pTau-association of ferritin light chain (FTL) for CBD but this did not identify cell types or pTau lesions.

We then selected a set of common or tauopathy-specific enriched proteins for further analysis with immunofluorescence and quantitative analysis. Represented in all tauopathies was the core retromer complex protein VPS35 that we found sequestered in several types of pTau lesions in all tauopathies.

We used AT8 phospho-tau (pTau) antibody as a probe to determine the composition of disease-specific pTau-associated proteomes in post-mortem frontal cortices of four major tauopathies in situ. We report 1,317 pTau associated proteins, with 409 being new, showing concordance with previous efforts.

To identify proteins that associate with tau pathology in these tauopathies, we developed an approach for in situ proximity labeling and isolation of aggregate-associated proteins using glass slides with standard 5μm thin formalin-fixed paraffin-embedded (FFPE) human brain tissue, termed ProPPr.

Here we addressed the great disease-specific morphologic and biochemical diversity of tau aggregates in Alzheimer’s disease (AD), corticobasal degeneration (CBD), Pick’s disease (PiD), and progressive supranuclear palsy (PSP), that remained to be explored with unbiased methods such as LC-MS/MS.

Probe-dependent Proximity Profiling (ProPPr) Uncovers Similarities and Differences in Phospho-Tau-Associated Proteomes Between Tauopathies - Molecular Neurodegeneration Background Tauopathies represent a diverse group of neurodegenerative disorders characterized by the abnormal aggregation of the microtubule-associated protein tau. Despite extensive research, the mec...

Our proximity proteomics study introducing Probe‑dependent Proximity Profiling (ProPPr) and applying it for profiling tau pathology‑associated proteins in tauopathies + @drneurochic.bsky.social & others is out now! @cziscience.bsky.social
molecularneurodegeneration.biomedcentral.com/articles/10....

A subcellular map of translational machinery composition and regulation at the single-molecule level Millions of ribosomes are packed within mammalian cells, yet we lack tools to visualize them in toto and characterize their subcellular composition. In this study, we present ribosome expansion micros...

“In neurons, RiboExM revealed a functional dichotomy between monosomes and polysomes. Monosomes were enriched in distal neurites at baseline, whereas polysomes formed dynamically in response to external stimuli…”

www.science.org/doi/10.1126/...

A new understanding of tissue biology from MS-based proteomics at single-cell resolution www.nature.com/artic...

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#proteomics #prot-paper

THE (real) single cell proteomics technique scSeq people love - NanoSplits- is out! Check out one of my favorite techniques of the last few years - the NanoSplits paper here!   The first preprint of this study is somewhere...

The nano splits paper is out! Single cell seq and single cell proteomics on the same single (one) normal sized human cell! proteomicsnews.blogspot.com/2024/12/the-...

Final episode of “what is a proteomics?” on #THEProteomicsShow with me and @proteomicsnews.bsky.social. This week we had Dorte Bekker-Jensen on talking about who can do proteomics. Obviously anyone, right?! Get it wherever you get fine podcasts.

Cryo-Et image of a brain extracellular vesicle

Benjamin Ryskeldi-Falcon’s @brfalcon.bsky.social group in the LMB’s Neurobiology Division, with Karen Duff's group at the UK DRI, have discovered that tau filaments are tethered to the membranes of extracellular vesicles in #Alzheimers

Read more: www2.mrc-lmb.cam.ac.uk/cryo-et-reve...
#LMBResearch

‘A place of joy’: why scientists are joining the rush to Bluesky Researchers say the social-media platform — an alternative to X — offers more control over the content they see and the people they engage with.

‘A place of joy’: why scientists are joining the rush to Bluesky

www.nature.com/articles/d41...

An impressive display of technologies was used to discover how cryptic #splicing causes #celldeath and leads to development of human #diseases.
www.science.org/doi/10.1126/...
It was a real joy to work together with @prieto-garcia.bsky.social and all other collaborators. Many thx 👍😀

Looks like PPS Silent Surfactant, Progenta anionic acid labile surfactant (AALS) I, AALS II, cationic acid labile surfactant (CALS) I, and CALS II are not commercially available anymore

Can someone please point us to a source for mass spectrometry compatible surfactants less pricey than Invitrosol, ProteaseMax, and RapiGest SF? DDM did not work that well in our hands for FFPE tissue extraction and proteomics.