Timo Rückert

Stepwise epigenetic signal integration drives adaptive programming of cytotoxic lymphocytes Lymphocyte differentiation during infection depends on the integration of antigen and cytokine signals, yet how the timing and sequence of these cues …

Excited to share my paper published today in @cp-immunity.bsky.social! We find that when CTLs integrate antigen and cytokine signaling, the timing of these cues matters - shedding light on an old question: Why do NK cells sometimes behave like CD8+ T cells?
www.sciencedirect.com/science/arti...

Less than one week left to apply 🔜

We are looking for motivated PhD candidates to study NK cell clonality and memory :)

Apply by December 10

Versatile and efficient non-viral integration of large transgenes in human T cells via CRISPR knock-in and engineered integrases Current gene transfer methods often lack the precision, versatility, or efficiency when integrating large transgenes, limiting the ability to engineer therapeutic T-cells with more complex payloads. H...

You like engineering T cells, but you are missing the right flavor?

Check out 'one-pot' PASTA recipe 🔥🍝: www.biorxiv.org/content/10.1101/2025.09.10.675267v1

We combined a unique mix of CRISPR knock-in with serine integrases for easy-to-adopt
𝐏rogrammable
𝐀nd
𝐒ite-specific
𝐓ransgene
𝐀ddition
🧪

Must read! Super exciting work and innovative approach.

📌

📌

📌

a baby is eating a piece of food while sitting on the ground . ALT: a baby is eating a piece of food while sitting on the ground .

Trying a slightly new model (for us at least) of #openscience. With @ronanchaligne.bsky.social and @karolisk.bsky.social, we pitted 10x Genomics Chromium GEM-X against Illumina PIPseq V head-to-head to understand these two platforms for single-cell genomics. Some thoughts off the bleeding edge: /1

📌

Reducing batch effects in single cell chromatin accessibility measurements by pooled transposition with MULTI-ATAC Large-scale scATAC-seq experiments are challenging because of their costs, lengthy protocols, and confounding batch effects. Several sample multiplexing technologies aim to address these challenges, b...

Happy to share that the preprint to our new scATAC-seq multiplexing method, MULTI-ATAC, is officially online:
www.biorxiv.org/content/10.1...

📌

📌

a cartoon says hey everybody an old man 's talking while bart simpson looks on ALT: a cartoon says hey everybody an old man 's talking while bart simpson looks on

Bluetorial: Avoiding becoming et al.

I decided not to do a postdoc with a Nobel laureate for personal reasons, but this decision had unanticipated benefits for my career.

📌

💥💥💥 !!! Please re-post !!! 💥💥💥

Hello Community,

We’re excited to announce that we’re moving to Groningen in January 2025 to embark on a new chapter in our research careers. For this, we need a house/flat and we hope to find a nice place with your help.

I'm incredibly honored to have received this recognition. A heartfelt thank you to everyone involved in making the evening so special :)

A short intro to the many new followers gained at 🦋:

🧪🔬we are a diverse team of researchers driven by curiosity, dedicated to understanding immune regulation in disease & cancer & exploring new strategies for immunotherapies 🩺⚕️

❤️ all things spatial; DCs, T cells, NK cells & single cell analyses.

We tackle these and other questions using different genomics and in vitro approaches applied, mostly analyzing human samples, so there's more to come hopefully soon!

Extrinsic and intrinsic drivers of natural killer cell clonality Clonal expansion of antigen-specific lymphocytes is the fundamental mechanism enabling potent adaptive immune responses and the generation of immune memory. Accompanied by pronounced epigenetic remod...

These findings raised a lot of questions on the factors driving activation, clonal selection, and persistence. We recently laid out some thoughts and perspectives in this review onlinelibrary.wiley.com/doi/10.1111/...

Clonal expansion and epigenetic inheritance of long-lasting NK cell memory - Nature Immunology Here, the authors use single-cell multiomics and profiling of mitochondrial mutations as endogenous barcodes to show that human adaptive NK cells induced by CMV persist as clonal expansions that inher...

To introduce myself and what I do: in my PhD @romagnanilab.bsky.social, I found that human NK cells maintain clonal immune memory to CMV infection www.nature.com/articles/s41...

Early emergence of T central memory precursors programs clonal dominance during chronic viral infection - Nature Immunology T cell memory formation is often described as occurring during the chronic phases of infection. Buchholz and colleagues use the phenomenon of ‘memory inflation’ following cytomegalovirus infection to ...

To populate my Bluesky and people know what I do: I am an MD turned immunology "basic" researcher. In the past I did adoptive T cell therapy and then fate mapping in the Buchholz lab in Munich:
CD8+ T cells in MCMV:
www.nature.com/articles/s41...

Postdoctoral Scientist (d/f/m) in Life Sciences/Genetics - Berlin (DE) job with Berlin Institute of Health in der Charité (BIH) | 12831190 The mission of the Berlin Institute of Health at Charité (BIH) is medical translation: transferring biomedical research findings into novel approac...

🚨 We’re hiring! 🚨
Join the Gaebler & Ibrahim Labs for an exciting postdoc position at the intersection of virology & gene regulation! Help us uncover mechanisms behind HIV latency using cutting-edge genome engineering. More info: www.nature.com/naturecareer...