Andi Brunner

Our work on NPC biogenesis is now out! Wanlu Zhang investigated the role of FG-Nups in NPC reassembly, pore dilation, and the nuclear envelope remodelling using acute depletions, correlative EM, and MINFLUX imaging 💫.
Thanks, Wanlu and all the collaborators!

Congratulations Wanlu!! 🚀🚀

YouTube video by EU Debates | eudebates.tv Mario Draghi’s Full Speech: Europe’s Harsh Reality — Powerless on the Global Stage! -ITALIAN-

Just got round to watching Mario Draghi's speech in Rimini in the original Italian. It is easily the most important speech on Europe so far this year and I hope someone (@grandcontinent.bsky.social?‬) publishes it in English and other EU languages soon.

www.youtube.com/watch?v=YMME...

Indeed as Ilya says, BrdU should only be added for a single S-phase. The longer you incubate with BrdU (the more replication cycles incorporate BrdU) the less available DNA you will habe after the RASER-FISH procedure :)

Nanoscale 3D DNA tracing in non-denatured cells resolves the Cohesin-dependent loop architecture of the genome in situ - Nature Communications A nanoscale 3D DNA tracing workflow visualizes Cohesin-dependent loops in single, structurally well-preserved cells. Computer simulations based on the tracing data give further insight into how Cohesi...

Our new non-denaturing nanoscale 3D DNA tracing technology provides single-cell (and sister-chromatid specific!) readout of chromatin structure with 10kb genomic resolution - Check it out!

www.nature.com/articles/s41...

Congratulations to Kai and Oyvind!!

Indeed difficult! Only the replicated strands are available for degradation, do ideal would be to BrdU-label in the S-phase prior to fixation. I guess for zebrafish embryos there is no uptake from outside?

BrdU incorporation during S-phase, UV-induced single-strand nicking after cell fixation and Exonuclease based digestion of the nicked DNA strand! :)

YouTube video by Rederei FM Igor Levit über Margot Friedländer beim Deutschen Filmpreis 2025 -

"Nicht einen Millimeter überlassen..."
Ich habe das jetzt erst gesehen und was soll ich sagen, was Igor Levit da anlässlich des Todes von Margot Friedländer aus dem Stand heraus sagt, ist das Berührendste, Wahrste und Richtigste, was ich seit ewig im deutschen Fernsehen gehört habe.

🎉 Excited to share our new work: “Adhesion-driven tissue rigidification triggers epithelial cell polarity”, now on @biorxivpreprint.bsky.social !

A huge thank you to @nicolettapetridou.bsky.social, Bernat, @crisp-c.bsky.social, Adrián, and everyone involved! 🙌

🧵⤵️

DWD-Fakten zur aktuellen #Trockenheit in #Deutschland: Noch nie seit Beginn der Auswertung 1931 war es in Deutschland im Zeitraum von Anfang Februar bis Mitte April so trocken wie 2025. Mehr Details auch zu #Europa in der heutigen Pressemitteilung unter www.dwd.de/presse

Spoiler: It is absolutely market manipulation

Our new findings on how chromosomes get ready for cell division are now published in @cellpress.bsky.social!

Congratulations, Kai, @andibrunner.bsky.social and everyone else involved! 🤩

www.sciencedirect.com/science/arti...

How chromosomes shape up for cell division | EMBL EMBL scientists have shown how overlapping loops of DNA stack upon each other in dividing cells to give rise to rod-shaped chromosomes

Did you know that DNA must form overlapping loops to give rise to the well-known X-shape of chromosomes? 🧬🧪

New research from EMBL's Ellenberg Group provides new insights into the process of chromosome formation.

@ellenberglab.bsky.social
@andibrunner.bsky.social

www.embl.org/news/science...

How chromosomes shape up for cell division | EMBL EMBL scientists have shown how overlapping loops of DNA stack upon each other in dividing cells to give rise to rod-shaped chromosomes

This has been an excellent collaboration together with Kai Beckwith in the @ellenberglab.bsky.social @embl.org. Thanks a lot to Kai, Jan, Natalia, and our collaborator Ralf Jungmann!!

A summary of the work can also be read at the @embl.org press release:
www.embl.org/news/science...

We thus propose that mitotic chromosome shape is dictated by Condensin-driven extrusion of long and overlapping loops up to a density set by chromatin self-repulsion.

Our model perfectly recapitulated our tracing data & the Condensin localization pattern as quantified recently in Walther et al. 2018.
rupress.org/jcb/article/...

Feature 1: Condensins pass each other. This leads to the formation of large, extensively overlapping loops with sizes up to 6-8 Mb.

Feature 2: DNA self-repulsion. This leads to self-organization and stacking of loops into the rod-shape.

Using a dynamic polymer constrained only by available quantitative Condensin chromatin-binding data, we could recapitulate the formation of native mitotic chromosome rods!

Two features were essential for that ->

We found that Condensins are also responsible for the backfolding of DNA at much larger genomic distances: up to 6-8 megabases.

Analyzing these traces, we found evidence for continuously growing loops, and our degron-based experiments showed that these loops are built by the Condensin complexes.

Kai and I used the recently established LoopTrace technology to trace the structure of single mitotic chromosomes in human cells in a very mild & structure pre-serving manner.

Mitotic chromosomes form through long, overlapping and self-repelling DNA loops, generated by the Condensin complexes!

Check out our newest paper here, and see below for a short Bluetorial!
www.cell.com/cell/fulltex...

SO excited to see this out!

We are #hiring!

We have two open postdoc/research scientist positions to join our #ERC funded project MITOFOLD to resolve the organization of mitotic chromosomes!

1) experimental biologist shorturl.at/LjGEM
2) data scientist shorturl.at/nDdhQ
Please share! 🙌

We look forward to your application! 🤩

History will remember this day— when an American President and Vice President abandoned all we stand for.

Zwei Tage vor der der Wahl haben Friedrich Merz und die Union eine Anfrage gestellt, die man in dieser Form sonst nur von der AfD kannte. In 551 (!) Fragen stellen sie die Gemeinnützigkeit zahlreicher NGOs in Frage, darunter auch des @netzwerkrecherche.org. dserver.bundestag.de/btd/20/150/2...

🇩🇪 ist die drittgrößte Volkswirtschaft der Welt. Morgen wählen wir - entweder für eine moderne, klimaneutrale Wirtschaft, oder für die Rückkehr zu Technologien des letzten Jahrhunderts. Lasst euch nicht für dumm verkaufen mit Scheinlösungen, die nur das fossile Geschäftsmodell noch etwas verlängern.

In today‘s talk on „Autocracy vs Democracy“ in Heidelberg, Prof. Dr. Aurel Croissant said that 7/9 landmark developments towards #autocracy have been made in the US.

A change back to more #democracy becomes increasingly unlikely.

At the same time, the white house posted this on X, citing #Trump.

YouTube video by Robert Habeck Zu meiner Dissertation | Robert Habeck

Ich rechne damit, dass heute, Vorwürfe gegen meine Doktorarbeit, die ich vor 25 Jahren geschrieben habe, veröffentlicht werden. Ich habe mich entschieden, das Ganze transparent zu machen. Denn ich kenne die Vorwürfe – und konnte sie vorab prüfen lassen.

www.youtube.com/watch?v=mYyA...

Erasure.
At the National Institute of Health, posters of women and scientists of color have been taken down. Shared by @ivancalcantara.bsky.social