Constantinos Patinios

Dr C. Patinios Awarded EMBO Installation Grant to Advance Genome Editing Research in Lithuania Vilnius University is a prestigious institution of science and studies in Lithuania, which develops world-class science and develops science-based international studies.

🔗 Discover more: bit.ly/3LUzM2U
#EMBO #GenomeEditing #CRISPR #SyntheticBiology #Microbiology #Biotechnology #Vilnius #LifeSciences

This work will drive new genome editing technologies and uncover fundamental DNA repair mechanisms, with impact across microbiome research, synthetic biology, industrial biotechnology, and healthcare.

🧬 My project, “Pause-Repair-Edit,” aims to move beyond current CRISPR tools by developing safer genome editing approaches that pause DNA replication to activate natural repair pathways—opening genome editing to many microorganisms that are currently hard to engineer.

🚀 Excited to share that I’ve been awarded an EMBO Installation Grant to establish my research group at the EMBL Partnership Institute, Vilnius University Life Sciences Center! The grant provides €50,000/year for 5 years to build an internationally competitive lab and advance genome editing research.

RNA-triggered Cas12a3 cleaves tRNA tails to execute bacterial immunity - Nature Cas12a3 nucleases constitute a distinct clade of type V CRISPR–Cas bacterial immune systems that preferentially cleave the 3′ tails of tRNAs after recognition of target RNA to induce growth arres...

Our paper is out! Hiding in plain sight among Cas12a nucleases, Cas12a3 cleaves not its RNA target but the 3′ ends of tRNA. Huge thanks to all who made this possible, especially the Beisel lab, Biao & Dirk for the structure, & @sebastianglatt.bsky.social for all things tRNA. doi.org/10.1038/s415...

Openness means more than access.

We’re pleased to announce that we now publish Replication Studies, helping make science more reliable, transparent, and trustworthy.
buff.ly/Gebypvq

Thank you Jens

Thank you Nicholas 😊

Amazing Martin!! All the best with the new lab and the amazing research you do!!!

Targeted DNA ADP-ribosylation triggers templated repair in bacteria and base mutagenesis in eukaryotes - Nature Biotechnology Append editing of ADP-ribosyl to thymine is used for precise modifications in bacteria and eukaryotes.

Targeted DNA ADP-ribosylation triggers templated repair in bacteria and base mutagenesis in eukaryotes - @helmholtz-hiri.bsky.social go.nature.com/4n7zDpG

Big shout-out to first authors Darshana Gupta, (myself :) ) & Harris V. Bassett, senior author Chase L. Beisel, and project initiator Scott P. Collins 🙌

This opens up brand-new possibilities for precision editing and studying DNA repair!

We developed append editing, a genome-editing strategy that installs ADP-ribose groups onto DNA to unlock new repair outcomes:
🔹 In bacteria → precise, scar-free edits via homologous recombination
🔹 In eukaryotes → unique T→A or T→C base substitutions not possible with current editors

Targeted DNA ADP-ribosylation triggers templated repair in bacteria and base mutagenesis in eukaryotes - Nature Biotechnology Append editing of ADP-ribosyl to thymine is used for precise modifications in bacteria and eukaryotes.

🚀 New publication alert! 🚀

We just published in @natbiotech.nature.com:
👉 Targeted DNA ADP-ribosylation triggers templated repair in bacteria and base mutagenesis in eukaryotes
doi.org/10.1038/s415...

The Laboratory of Dr. Constantinos Patinios VU Gyvybės mokslų centras jungia tris VU padalinius – Biomokslų, Biotechnologijos ir Biochemijos institutus. Centras įkurtas vystant integruotą mokslo, studijų ir verslo centrą (slėnį) „Saulėtekis“.

Learn more ➡️ www.gmc.vu.lt/en/lsc-embl/...

Deadline: Open until a suitable candidate is found.
📧 Send your CV + Motivation Letter to: constantinos.patinios@gmc.vu.lt

🧬 Our work focuses on next-generation genome editing tools for bacteria, yeasts, and phages — with a special focus on DNA replication stalling and repair!

🌍 Based in beautiful Vilnius, Lithuania
🔬 Access to state-of-the-art equipment and facilities
💼 Competitive salary, personalized mentorship, and cutting-edge research!

If you’re passionate about genome editing or synthetic biology…
If you want tailored mentorship to drive independent research and launch your career…
This is the place for you! ✨

🚀 Join the Patinios Lab at the VU LSC - EMBL Partnership Institute for Genome Editing Technologies!
We are looking for a Postdoctoral Researcher to join our growing team! 🔎

Good news on this 35th anniversary of the #Restoration of #Independence in #Lithuania!

A new ERC project from a Lithuanian researcher can now go ahead, as they were able to secure alternative EU funding for her ERC Consolidator Grant.

How did this happen? Find out in the thread 🧵👇

Excited to see our SIBR work out in Trends in Biotechnology :)
doi.org/10.1016/j.ti...

This is the first study I have coordinated from start to finish, and it brings me immense satisfaction to see it published.

For a brilliant description of the tech check this: www.linkedin.com/feed/update/...

Huge thanks to Simona Della Valle, Enrico Orsi and Sjoerd Creutzburg for driving this work.

Happy editing :)

Google Colab

If you want to use SIBR2.0 for your gene/protein of interest, you can visit the SIBR Site Finder site: colab.research.google.com/drive/162gIZ...

SIBR2.0 can virtually control the expression of any gene/protein of interest, in a wide range of (if not all) mesophilic prokaryotes

By using SIBR2.0-Cas12a we achieved ~70% editing efficiency, in just 48h after electroporation

We show that SIBR2.0 is great for inhibiting the translation of the T7 RNAP in E. coli and then we used it to tightly and inducibly express the Cas12a protein in C. necator

his means that even with the alternative translation site present in the SIBR sequence, a non-functional and split protein will be produced

This is where magic happened!! We developed SIBR2.0 :)
SIBR2.0 includes the introduction of SIBR within the coding sequence of the gene of interest, splitting it into 2 distinct exons

The alternative site for translation appeared to be gene- and organism- specific