Theo Mantamadiotis

Delighted to present our research at the #Spatial Imaging Workshop, hosted by Biological Optical Microscopy Platform (BOMP) @ The University of Melbourne & supported by Leica Microsystems & Light Microscopy Australia. The multiplex IHC image shows cell signaling activity using phospho-Abs in #GBM

The Who's Who of spatial biology in Australia & SE Asia at the WEHI Spatial Technology Symposium in Melbourne. A fabulous lineup of speakers & platform leaders highlighting the fast moving research & tech in #spatial #biology

The two newest members of the Cure Brain Cancer Foundation, Scientific Advisory Committee

I’m delighted to join the Cure Brain Cancer Foundation Scientific Advisory Committee & look forward to working with the CBCF, consumer advocates & researchers, to advance our understanding of the mechanisms underpinning brain cancer, to ultimately help patients and their families.

T

Multiomics Blueprint of Extreme Human Lifespan: “The picture…emerges…that extremely advanced age & poor health are not intrinsically linked & that both processes can be distinguished & dissected at the molecular level”

Santos-Pujol et al., doi.org/10.1101/2025...

Vaccine-induced T cell receptor T cell therapy targeting a glioblastoma stemness antigen - Nature Communications Vaccination in glioblastomas does lead to the emergence of tumour-antigen-specific T cells but T cell dysfunction, poor tumour infiltration and persistence hinder efficient tumour killing. Here author...

🚀 New publication! We developed a PTPRZ1-specific TCR-T cell therapy for glioblastoma.

🔬 Key findings:
✅ Identified a PTPRZ1-reactive TCR from a vaccinated patient
✅ Kills glioblastoma cells with no off-target effects
✅ Eliminates tumors in murine brain models

🔗 www.nature.com/articles/s41...

The University of Melbourne, Brownless Biomedical Library this morning & as it was: For me, it was the place to leaf through the latest hardcopy journal issues on the display stands, or search the journal archives for that elusive article required for my thesis

#GBM #TME #spatial analysis: incredible #cellsignaling heterogeneity at single cell resolution, predicts cell function. Differential PI3K-MAPK-CREB pathway activation/co-activation correlates with proliferation, cell density, angiogenesis & proximity to ECM-rich stroma: see go.unimelb.edu.au/52z8

While updating my CV for another grant application, I am yet again amused by the title of our review article (PDF version), from 2011 (the title is correct on PubMed & the journal web site). I never followed up to fix this because it's great as it i; at the cutting edge of science & mythology:

Dynamic effects of bilingualism on brain structure map onto general principles of experience-based neuroplasticity - Scientific Reports Scientific Reports - Dynamic effects of bilingualism on brain structure map onto general principles of experience-based neuroplasticity

Bilingualism is associated with a bigger brain. Similar to other cognitively demanding skills, it leads to dynamic subcortical structural adaptations which can be nonlinear, in line with expansion-renormalisation models of experience-dependent neuroplasticity. www.nature.com/articles/s41...

Gliomagenesis mimics an injury response orchestrated by neural crest-like cells - Nature A study using glioblastoma mouse models, serial magnetic resonance imaging and single-cell profiling details changes in the identity and balance of cellular states from initiation of tumorigenesis to ...

Great story; Gliomagenesis mimics an injury response orchestrated by neural crest-like cells www.nature.com/articles/s41...

Fascinating study!

Merry Christmas!
Καλά Χριστούγεννα!
Frohe Weihnachten!

Unraveling the functional complexity of the tumor microenvironment via #spatialbiology. The 2 images show rGBM tissue with cell signaling cell plot (left) & cell-type mIHC (right). Neoplastic, vascular & immune cells show distinct signals which correlate with cell clustering & proximity to ECM

In Carcinogenesis: shorturl.at/6pvy6 Male mice with nestin-targeted PI3K & PTEN mutations develop tumors which resemble an aggressive form of sex cord stromal tumors. We describe the cell-of-origin, the complex histopathology & using #spatialbiology, the immune cell infiltration of this rare cancer

Toward precision immunotherapy using multiplex immunohistochemistry and in silico methods to define the tumor immune microenvironment - Cancer Immunology, Immunotherapy Recent developments in cancer immunotherapy promise better outcomes for cancer patients, although clinical trials for difficult to treat cancers such as malignant brain cancer present special challeng...

Links to the papers:

doi.org/10.1007/s134...

doi.org/10.1038/s414...

doi.org/10.1007/s002...

doi.org/10.1016/j.ad...

doi.org/10.1016/j.im...

Well done Samuel! Last week, Samuel Widodo submitted his PhD thesis: "Signalling and Transcriptional Regulation of Tumour Associated Macrophage Polarisation in Brain Cancer" @UniMelb & has published 12 papers, so far, with some shown here. #glioblastoma #GBM #spatialbiology #OMICS #macrophages

Wow! That is an amazing body of work. Well done!

Thanks Ioanni! As a keen & active SpatialBiol user, I’d be happy to added on your SpatialBiol list

Whole-brain spatial transcriptional analysis at cellular resolution Recent advances in RNA analysis have deepened our understanding of cellular states in biological tissues. However, a substantial gap remains in integrating RNA expression data with spatial context acr...

Amazing: 3D organ-wide spatial analysis. (Almost) all of it: real-time methods for 4D analysis next? www.science.org/doi/10.1126/...

HE stained testis exhibiting a sex cord stromal-like tumor on the left & in the right, a spatial map showing tumor & immune cells (see color key)

Mice with an inducible nestin-CreER transgene targeting PI3K & PTEN develop aggressive brain tumors doi.org/10.1093/neuo... ~20% of the mice also show tumours affecting the reproductive organs. Using #spatialbiology we describe the testis tumors, in the bioRxiv pre-print: doi.org/10.1101/2024...

To learn about #braincancer & the innovative use of mIHC #spatialbiology tech to understand key molecular & cellular mechanisms regulating the #tumormicroenvironment, go to: shorturl.at/Nafcw #GBM #brain #cancer

1st Bsky post pushed from you know where - Following several presentations on how we use #spatialbiology to investigate the #immunology of #braincancer - also see our recent review on this topic shorturl.at/7pwV1