Andrea Caldarola

Google AI search reports C32 melanoma cells are grown at 32 degrees C because they have C and 32 in their name

PhD-level intelligence

I am genuinely impressed by large language models - they can absorb disparate components of text into some consolidated view, they can produce extremely good language and - with the right model - translate pretty well between languages and they are an excellent text based UI for humans to use. But..

A Photoswitchable HaloTag for Spatiotemporal Control of Fluorescence in Living Cells Photoswitchable fluorophores are critical for advanced bioimaging. Here, we develop a photoswitchable self-labeling HaloTag that can reversibly modulate the emission of a bound fluorogenic dye via a ....

Our photoswitchable HaloTag is out in Angewandte Chemie @angewandtechemie.bsky.social ! We now also show that this system can be used to control emitter density in SMLM. Congrats to Franzi, Bego, and our amazing collaborators. Check it out: onlinelibrary.wiley.com/doi/full/10....

This #microscopymonday is a cleaning day. Watch how a #microglia cell mops up and phagozytoses debris and junk in a very messy neuronal co-culture. BTW, it was a proper cleaning job → the neurons made it and formed nice networks
Phase contrast imaging started at DIV1. One frame per 20 min.

Optogenetic control of PLC-γ1 activity polarizes cell motility https://www.biorxiv.org/content/10.1101/2025.10.09.681531v1

Right wing populist government does something to pander to their voter base while being of no actual benefit to anyone, many such cases!

For #FluorescenceFriday : 3 cells start at the same Ca2+ concentration but undergo 3 different Histamine responses. Imaged using the lifetime-based sensor Tq-Ca-FLITS.

Optogenetic construction of de novo integrin-adhesion complexes reveals role for biocondensation in adhesion nucleation https://www.biorxiv.org/content/10.1101/2025.10.08.681107v1

Cabinet to make 10 years minimum to obtain Dutch citizenship According to the government, the Dutch cabinet plans to double the minimum residency requirement for naturalization from five to 10 years, extending the time foreigners must live legally and continuou...

“We are making sure that people have a stronger bond with our Kingdom and are well integrated and able to fully participate in society before becoming Dutch citizens” : Dutch society is obviously too alien & bizarre for a silly foreigner to integrate within 5 years, we'll take your tax money though!

Paper in Nature 150 years ago. Beats reading any single-cell experiment…

Optogenetic storage and release of protein and mRNA in live cells and animals - Nature Communications Spatiotemporal control of biomolecules is a key challenge in cell biology. Here, authors introduce RELISR, an optogenetic condensate system enabling reversible, light-dependent sequestration and relea...

Optogenetic storage and release of protein and mRNA in live cells and animals | Nature Communications share.google/gz8nYXZ3hUVh...

#spatialbiology #imagingtools #fluorescencemicroscopy

Also a great supervisor!! In my entirely unbiased opinion

Pretty nice

Three-photon in vivo imaging of neurons and glia in the medial prefrontal cortex with sub-cellular resolution - Communications Biology Three-photon in vivo imaging provides access to medial prefrontal cortex, hippocampus and spinal cord in mice at up to 1.7 mm depth of neurons, dendrites, spines, astrocytes and microglia.

It is out! Read how you achieve 1.7 mm deep 3-photon imaging in prefrontal cortex with dendritic spine resolution over a week.
www.nature.com/articles/s42...

High-performance genetically-encoded green and red fluorescent biosensors for pyruvate Pyruvate is the end-product of glycolysis and a central metabolite involved in many biochemical pathways. However, a lack of high-performance (i.e., Δ F / F > 10) single fluorescent protein (FP)-base...

New tools! GreenPy and ApplePy are fluorescent biosensors for pyruvate with huge responses (20–40x in vitro) and a range of affinities (10s of μM to mM). These should be game changers for imaging of metabolism! Great work by Shosei Imai @sikmys.bsky.social and team 👏. www.biorxiv.org/content/10.1...

president tweeted "no hot dog" and i lost $5 trillion dollars but today he tweet "hot dog back" and i got 5 trillion hot dog

Nonlinear sound-sheet microscopy: Imaging opaque organs at the capillary and cellular scale Light-sheet fluorescence microscopy has revolutionized biology by visualizing dynamic cellular processes in three dimensions. However, light scattering in thick tissue and photobleaching of fluorescen...

Sound-sheet microscopy - in this study, we introduce nonlinear sound-sheet microscopy (NSSM), a method to image targeted biological functions across cubic centimeters of opaque living tissue.

www.science.org/doi/10.1126/...

Fast & Fair peer review A new initiative offering high-quality peer review within 7 working days of submission Biology Open

We are excited to be launching the next phase of our Fast & Fair peer review initiative: offering high-quality peer review within 7 working days. #fastandfairpeerreview
Read the Editorial by EiC Daniel Gorelick @danielgorelick.bsky.social at: bit.ly/4kYD1mL

So happy I finally made the ILID-SspB Optogenetic system work in 🐸. Though not perfect and need some optimisations, it worked. Got the constructs from @joachimgoedhart.bsky.social and made it into a frog expression system. Here is the Opto-PH-Rho in action. It's real, guys!! #FluorescenceFriday

Dear all, Because of your help shining light on this all 15 were reinstated today. This is a huge win for science - When We Fight We Win!!!

magneticScarlet3

📢 #Preprint Alert: For #fluorescentfriday we report minimally-sized photoactivatable PaX dyes with red and far-red emission for #STED, #SMLM, and multiplexed #MINFLUX #SuperResolution #Microscopy! A huge thanks to the Optical #Nanoscopy team at @mpi-mr.bsky.social! 🔬

www.biorxiv.org/content/10.1...

Gen Z from lab, reflecting wistfully upon the passage of time in grad school: “dang rip”

A million times the man that Trump or Putin will ever be.