Jalal Uddin

Large-scale CRISPR screening in primary human 3D gastric organoids enables comprehensive dissection of gene-drug interactions Nature Communications - CRISPR-Cas9-based screens have allowed the study of gene-drug interactions. Here, the authors develop CRISPR-Cas9 knock-out, activation and repression screens in human...

rdcu.be/eA0FK

Congrats to postdoc Zhe Zhou on her paper out today in @cp-cellhostmicrobe.bsky.social showing that gut bacteria cross-feed a common dietary antioxidant to produce energy under anaerobic conditions. Thanks to collaborators Angela Jiang and Xiaofang Jiang at NIH.
www.sciencedirect.com/science/arti...

Gas Phase Separation of Modified Peptides for Activity-Based Protein Profiling Profiling the reactivity of the proteome with amino-acid level resolution requires the identification and quantification of reacted peptides in the presence of abundant unmodified peptides. Affinity-b...

An inspiring paper by the group of Jacob Geri in Analytical Chemistry. They present a method for residue-specific #chemoproteomics without the need for an enrichment step. To achieve this, they developed reagents that shift the ion mobility for gas phase separation. pubs.acs.org/doi/full/10....

Probing the proteome - Nature Biotechnology Chemical proteomics has brought rigor to covalent drug discovery and drugs to the clinic. Can it deliver a new generation of drug targets?

A new exciting article in @natbiotech.nature.com highlights the power of chemical proteomics to find druggable pockets on difficult high-value targets. Great insights into applications in covalent and non-covalent drug discovery including clinical development. (1/5) www.nature.com/articles/s41...

Congratulations to Dr. Stavroula Hatzios on this well-deserved recognition! 👏

Activity-Based Protein Profiling of Serine Hydrolases in Bacteria: Methods and Protocols Activity-based protein profiling (ABPP) is a chemoproteomic technique that uses functionalized covalent enzyme inhibitors known as activity-based probes (ABPs) to detect, quantify, and identify active...

Pleased to share our lab-optimized chapter in Methods in Molecular Biology: “Activity-Based Protein Profiling of Serine Hydrolases in Bacteria.” Thanks to @christianslentz.bsky.social .
doi.org/10.1007/978-...

Identification of covalent inhibitors of Staphylococcus aureus serine hydrolases important for virulence and biofilm formation - Nature Communications Staphylococcus aureus is a leading cause of bacteria-associated mortality worldwide. New tools are needed to both image and treat this pathogen. We previously identified a group of S. aureus serine hy...

Our work identifying new covalent inhibitors of Staphylococcus aureus serine hydrolyses from fragment libraries is out! great work from @tulsiupadhyay.bsky.social and our many collaborators, including @christianslentz.bsky.social, @mfellnerlab.bsky.social. #chembio
link.springer.com/article/10.1...

Chemical strategies for targeting lipid pathways in bacterial pathogens Microbial pathogens continue to plague human health and develop resistance to our current frontline treatments. Over the last few decades, there has b…

If you are interested in lipids in bacteria, check out this awesome new review written by my talented student Alyssa Carter, super postdoc Emily Woods and me. #chembio
www.sciencedirect.com/science/arti...

Identification of a secreted protease from Bacteroides fragilis that induces intestinal pain and inflammation by cleavage of PAR2 Protease-activated receptor 2 (PAR2) is a central regulator of intestinal barrier function, inflammation and pain. Upregulated intestinal proteolysis and PAR2-signaling are implicated in inflammatory ...

Excited to report our studies that identified a new serine protease secreted by the gut microbe Bacteroides fragilis that regulates pain and inflammation through PAR2 activation. #ChemBio. Check it out!https://www.biorxiv.org/content/10.1101/2025.01.15.633241v1

Thanks a lot @monaj66.bsky.social 😊

Our paper. Thanks a lot @christianslentz.bsky.social 😊