Michalis Averof

We'll be looking for a postdoc to bring these approaches together, funded for 3+ years. If you are interested please get in touch; if you know someone who might be interested please share this message. 3/3

for light sheet microscopy w. Flamingo team @metteh-thorsager.bsky.social, genetic barcoding w. @mgrillo.bsky.social, and a video game for cell tracking w. @jytinevez.bsky.social. We'll apply these tools to discover the progenitors for sense organ regeneration in Parhyale. 2/3

We've just been awarded a grant to study the cellular basis of regeneration – to track the progenitors of sensory organs in the context of leg regeneration, in our favourite crustacean tinyurl.com/parhyale, based on live imaging and cell tracking. The project involves some cool collaborations... 1/3

including "panoramic" key regulators, a new term in the hype toolkit ;)

John Maynard Smith on natural history.

(London Review of Books, April 1982)

diverse body shapes of Trichoplax, from small pancake to irregular spaghetti

Expression of clock genes tracks daily and tidal time in brains of intertidal crustaceans Eurydice pulchra and Parhyale hawaiensis Intertidal organisms, such as the crustaceans Eurydice pulchra and Parhyale hawaiensis, express daily and tidal rhythms of physiology and behavior to …

In the brain of intertidal crustaceans (Eurydice and Parhyale), circadian clock genes are expressed with a daily rhythm (24h) in some cells and a tidal rhythm (12.4h) in other cells. Two different clocks working in the same brain, using the same components.

I think you can also get eduroam in swedish airports

Trichoplax adhaerens is one of the simplest and most enigmatic animals on earth. Its body shows incredible fluidity, changing shape within minutes. In a recent visit by Andrea Pasini we had the chance to host and observe these amazing animals live.
[movie accelerated 3x; animal 0.5 to 1 mm in size]

Cartoon of autonomous and light-modulated spwning cycles in Clytia IZ-D

Intriguing findings from our Sendai collaborators Ruka Kitsui and Ryusaku Deguchi. While C. hemispherica spawns at dawn, Clytia IZ-D species spawns at dusk. This is not triggered by dark but, unexpectedly, by light-mediated extension of an autonomously-running 20h cycle. doi.org/10.1101/2025...

"To show our solidarity with our colleagues in the United States who live in fear, and to raise awareness of the abuses that are taking place, fortunately on a much smaller scale but still worrying, in Europe and France."

#StandUpForScience Lyon France

Yes, one can count many scientists among those who fought fascism. I like the example of Monod because he was an active researcher at the same time as he participated in the resistance. I think Jacob fought in WWII and became involved in research later.

I prepared these as banners for the #StandUpForScience demonstration on Friday. Happy to share the high-res files, if anyone wants to use them.

Do you have experience in generating transgenic tools and fluorescent markers? Are you interested in developing new tools in a non-conventional model, to observe and genetically manipulate cells during leg regeneration? We are recruiting!

www.averof-lab.org/pages/3560

Nothing to do with martyrdom or boys clubs, just remembering how others coped in the darkest days of fascism at different times. With you for focusing on the present.

Rita Levi-Montalcini defied fascism, which excluded her from university due to her jewish ancestry, by setting up a clandestine lab in her own bedroom. During 1940-42 she carried out experiments that set the foundations for her Nobel-prize-winning research a decade later.
nautil.us/a-lab-of-her...

yes, nice book, which also describes Monod's connection with Albert Camus

As the world of science wakes up to modern fascism, it's worth remembering older generations who stood up for humanity in much harsher circumstances. Jacques Monod worked actively for the resistance in WWII, while pursuing early stages of his research in occupied Paris. @standupforscifr.bsky.social

you can actually download the contents of your account before deleting

Do you write hemoglobin or haemoglobin? The greek "αι" used to be transcribed as "ae", but lately it's simplified to an "e". A matter of taste I guess.

😂 that's true

Please, show me your boundaries - the Node from Irene Karapidaki, Béryl Laplace-Builhé and Michalis Averof What is this? These are crustacean embryos injected with mRNA encoding a red fluorescent

thenode.biologists.com/please-show-...

and please do encourage anyone else interested (Woods Hole people etc.) to write to me – I'm happy for everyone use these constructs, whether they join our screen or not

We don't have anyone doing zebrafish yet, so that would be great! Please send me an email.

If you can you deliver mRNA into those cells, screen should be easy (if not, you'll have to transfer the constructs into an appropriate vector). Send me an email if you'd like me to send you the constructs.

Happy to send you the constructs and discuss if you want to join us in screening these tags across species. Please email me.

Current work by a great team: Irene Karapidaki (Parhyale), Tsuyoshi Momose (Clytia) and Marie Zilliox (light sheet microscopy). Grateful for support from the Agence Nationale de la Recherche and the Fondation pour la Recherche Medicale.

Plasmids available at www.addgene.org/browse/artic... 4/4

Would you like to join us in screening these tags in diverse species? Please get in touch. We're happy to share the constructs for generating mRNA, and will be happy to include new species –and new co-authors– in our comparative study. 3/4

The tags employ different mechanisms for plasma membrane localisation, including prenylation, myristoylation, palmitoylation, GPI anchoring, lipid-binding domains, signal peptides and transmembrane domains for trafficking to the plasma membrane. All fused to mScarlet3 FP. 2/4