katja bekcic

1H R1ρ Relaxation Identifies a Hidden Intermediate in DNA Base-Pairing 1H R1ρ Relaxation dispersion (RD) NMR experiments provide valuable atomic-level insights into transient, high-energy conformational states of biomolecules. However, cross-relaxation artifacts can hamp...

new #DNA #excitedstate just dropped💥 when my colleagues investigated how to avoid cross-relaxation artifacts in proton R1ρ experiments, which are used to measure #dynamics of #biomolecules, they discovered an excited state of DNA that may have a role in binding anticancer drugs
🔗 bit.ly/4o5m7U2

have you ever worried about your DNP MAS #solid-stateNMR experiments on #nucleicacids being too sensitive? well my colleagues have, so they used site-directed spin labelling to link a DNP-active biradical to RNA, which allows enhancement of the RNA but not pesky background signals!
🔗: bit.ly/4nmAl2K

Optimising in-cell NMR acquisition for nucleic acids - Journal of Biomolecular NMR Understanding the structure and function of nucleic acids in their native environment is crucial to structural biology and one focus of in-cell NMR spectroscopy. Many challenges hamper in-cell NMR in ...

Super cool #RNAdynamics have been discovered by NMR. Next, we want to observe these dynamics in their native environment with #in-cellNMR. Current limitations include low sensitivity. My colleagues help address this by measuring selective T1 to shorten acquisition time.
🔗 bit.ly/4doL8FD #scicommUU25

Base-pair conformational switch modulates miR-34a targeting of Sirt1 mRNA - Nature Repression of a messenger RNA by a cognate microRNA depends not only on complementary base pairing, but also on the rearrangement of a single base pair, producing a conformation that fits be...

Here is a phenomenal example of why we care about #RNAdynamics. My group shows an excited state of a miRNA:mSirt1 complex is involved in downregulation of Sirt1, a tumour promoter. Identifying these excited states can help guide design of miRNA mimics to treat cancer.
🔗 bit.ly/4kcBKI4 #scicommUU25

RNA Dynamics by NMR Spectroscopy RNA is a flexible molecule: This is what makes it functional, but also difficult to study, especially when the goal is to access those dynamic, often low-populated and short-lived conformers. Here th...

One misconception about RNA is that it’s static. RNA is actually ✨dynamic✨ and exists as a ground state in exchange with higher energy excited states. These #RNAdynamics allow RNA to perform functions and can be studied at the atomic level using NMR. Read more here 👉 bit.ly/43kbBQ3 #scicommUU25

Hi BSKY!💫 I'm Katja, a 2nd year PhD student at Uppsala University in the Petzold group. My projects focus on improving #in-cellNMR of RNA & using #solid-stateNMR to investigate RNA:protein interactions #scicommUU25