Hugo Brandao

This is great, thank you!

16.12.2024 19:00 — 👍 1    🔁 0    💬 0    📌 0

Fascinating work! Question: from the two example traces, it seems the time intervals become longer with each successive SMC step. Is the rate of extrusion dependent on the DNA torsion? Or, would any slowing down be attributed to the tension (i.e., DNA stretching) imparted by the magnetic tweezers?

14.12.2024 05:46 — 👍 0    🔁 0    💬 2    📌 0

All eukaryotic SMC proteins induce a twist of −0.6 at each DNA loop extrusion step Measuring single DNA loop extrusion steps of individual eukaryotic SMCs revealed that ATP binding induces a DNA twist of −0.6.

Today, we report a new discovery about this - a new twist to the story!

These SMC motor proteins do not only extrude the DNA into a loop. While doing so, they also put TWIST into the extruded DNA loop!

See our paper today in Science Advances: www.science.org/doi/10.1126/...

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13.12.2024 19:13 — 👍 43    🔁 9    💬 3    📌 0