Jeff Mold

It’s why I got on twitter then here - to try and promote visibility of my own science and more often others work I really liked

Visibility. I think at the end of the day that’s what everyone I know cares most about. That’s the power of the profit journals. People read and share them. They are good at marketing

What’s the best generalist non-profit? Does Science count?

The strain on scientific publishing Abstract. Scientists are increasingly overwhelmed by the volume of articles being published. The total number of articles indexed in Scopus and Web of Science has grown exponentially in recent years; ...

If I could change one thing about #ScientificPublishing I'd ask funding bodies to stipulate all work they fund be published in non-profit journals.

The knock-on effects would alleviate most of the strain on #AcademicSky.

This isn't hard. It's big, but actually, it's pretty easy.

1/n

I wish profit journals would turn into places which only published very good overviews/reviews of the most interesting trends in science highlighting preprints and providing a historical perspective

I think it takes incredible talent to get to a place where some luck comes into play… think of tom Brady. He may not be tom Brady if not for ending up on the patriots and the starting qb getting hurt at the right time. But he is immensely talented

🤣 I see why it’s hard to digest

One of the best English sentences ever written - anybody got others?

For a transitory, enchanted moment man must have held his breath in the presence of this continent, compelled into an aesthetic contemplation he neither understood nor desired, face to face for the last time in history with something commensurate to his capacity for wonder”

This is human testes stained with an antibody of unknown specificity hence my question. Are there neurons that live here?

What sorts of largeish cells live in between the semineferous tubules at about 1-10% frequency like this:

Escherichia coli with a 57-codon genetic code The near-universal genetic code uses 64 codons to encode the 20 canonical amino acids and protein synthesis. Here we designed and generated Escherichia coli with a 4 Mb synthetic genome in which we re...

Love this lab’s work www.science.org/doi/10.1126/...

YouTube video by Science Communication Lab Susan Lindquist (Whitehead, MIT / HHMI) 3: Prions: Protein Elements of Genetic Diversity

Even better one: m.youtube.com/watch?v=pgVs...

Intratumoral Treg ablation is sufficient to mediate tumor control systemically without autoimmunity Regulatory T cells (Tregs) infiltrate most tumors, yet whether they suppress immune responses directly within tumor tissues is untested. We used intratumoral (IT) delivery of diphtheria toxin (DT) in ...

www.biorxiv.org/content/10.1...

This looks like a fascinating study (and well done) www.biorxiv.org/content/10.1...

It’s cause people don’t read the papers. They read the abstract or just the title. It’ll get worse with AI

Hence why fetal cd4s produce abnormal tnf. Who knows why…

*fetal

Fetal and adult hematopoietic stem cells give rise to distinct T cell lineages in humans - PubMed Although the mammalian immune system is generally thought to develop in a linear fashion, findings in avian and murine species argue instead for the developmentally ordered appearance (or "layering") ...

My take home from reading this is the same as my old paper - feral and adult T cells are fundamentally different and comparing individual features is difficult.. pubmed.ncbi.nlm.nih.gov/21164017/

The notion that fetal treg express less foxp3 or cd25 or that there are substantial numbers of non-treg effector/memory cells in fetal tissues certainly is at odds with what we saw in our previous work. Maybe tissue specific differences. I’d like to see a cd4 vs foxp3 stain scatterplot here

For example see the overview of the data: what is the odd population only seen in fetal cd4?! (My guess fetal tregs). And there’s barely any signs of effectors/memory

I’m not a big fan of mass spectrometry results shown as heatmaps and UMAPs. I think lots of cytof data I’ve seen (and generated) have difficult to interpret differences between positive and negative signals but maybe that’s just me…. But beware the dead cells in fetal cultures…

The reason why naive T cell activation by alloantigen leads to a large treg expansion is partly because we saw the tregs had a survival advantage over the non treg. We also found the fetal treg were highly stable and very good at dividing in vitro with IL2.

This is a bit of weird analysis - and looking through it I don’t necessarily recall seeing the same stuff. Fetal effector T cells are prone to death in vitro and there are relatively few in vivo. Most are treg (foxp3+) www.pnas.org/doi/10.1073/...

One of the best lecturers and most creative thinkers I’ve encountered on this earth. I love her explanation of prion biology

YouTube video by iBiology Techniques Prion Disease - Susan Lindquist (MIT/HHMI)

www.youtube.com/watch?v=60H5...

High-resolution spatial mapping of cell state and lineage dynamics in vivo with PEtracer Charting the spatiotemporal dynamics of cell fate determination in development and disease is a long-standing objective in biology. Here we present the design, development, and extensive validation of...

I generally like anything these folks do - but it usually takes me a week to digest it. This one looks really nice too. And lung cancer to boot.

www.science.org/doi/10.1126/...

My grandfather was one of the scientists in charge of identifying carcinogens in tobacco smoke in the 1960s and 70s in the employ of cigarette companies… www.sourcewatch.org/index.php/Ja...

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There’s an incredible story about that in the later part of this book: www.audible.co.uk/pd/B00PR0ERU...

How has the data held up on tobacco carcinogens as adducts that induce hotspot mutations? I’ve always thought the larger culprit was general cellular stress caused by generic rather than specific damage from smoking