Paraskevi Kritsiligkou

Global analysis of translocon remodeling during protein synthesis at the ER - Nature Structural & Molecular Biology The authors use selective ribosome profiling to define how and when factors for N-glycosylation and membrane insertion engage and disengage from the core Sec61 translocation channel during biogenesis ...

This was a fun new collaboration using selective ribosome profiling to understand how the subunit composition of the ER translocon is tailored by the nascent chain. Congratulations to everyone involved!

www.nature.com/articles/s41...

YouTube video by EMBO - excellence in life sciences How to apply for an EMBO Postdoctoral Fellowship: Tips from the Committee

Want to apply for an EMBO Postdoctoral #Fellowship? 🧪

Here are tips from the EMBO Fellowship Committee:
www.youtube.com/watch?v=xY7c...
#LifeSciences #grant #funding

Translation landscape of stress granules Cryo–electron tomography visualizes stress granules in situ, revealing their spatial interplay with translation machinery.

What's the role of stress granules in translation? Here we provide a clue: we characterized the distribution of ribosomal complexes within SGs and reproduced this landscape with a phase-field model, pointing to a new regulatory mechanism. #TEAMTOMO www.science.org/doi/10.1126/...

🚀 Our new paper is out @natmethods.nature.com!

Kuffer & Marzilli engineered conditionally stable MS2 & PP7 coat proteins (dMCP & dPCP) that degrade unless bound to RNA, enabling ultra–low-background, single-mRNA imaging in live cells.

🔗 www.nature.com/articles/s41...
🧬 www.addgene.org/John_Ngo/

Mapping the genetic landscape of iron metabolism uncovers the SETD2 methyltransferase as a modulator of iron flux Genetic screens using endogenous reporters map key modulators of cellular iron, including the transcriptional regulator SETD2.

Happy to share latest work from the lab. Led by talented first author @awmartinelli.bsky.social. Mapping the genetic landscape of iron metabolism uncovers the SETD2 methyltransferase as a modulator of iron flux | Science Advances www.science.org/doi/10.1126/... Congratulations to all the team.

Rapid remodeling of NTP levels enables immediate translational adaptation to energy stress in yeast Bexley et al. report that rapid metabolic shifts mediate immediate, protective changes in RNA metabolism following energy depletion. In yeast, protein production is regulated by tuning enzymatic const...

www.cell.com/molecular-ce...

#RNAsky
💜💜💜💜💜💜💜

It was a super cool talk 🎉

On my way back from a truly brilliant yeast meeting! With more than 500 participants, so much cool science and loads of fruitful discussions, this week was remarkable! #yeast2025

I am in Paris this week for #yeast2025!

Lipoyl deglutarylation by ABHD11 regulates mitochondrial and T cell metabolism Nature Chemical Biology - Glutarate rewires cell metabolism through conjugation to proteins or fatty acids, but how glutarylation is regulated is unclear. ABHD11 was identified as a...

Delighted to share our latest work on ABHD11 as a novel deglutarylating enzyme, and its role in T cell metabolism @natchembio.nature.com rdcu.be/ewiYZ. Many congratulations to co-first authors Guine Grice, Eleanor Minogue and @hudsonwilco.bsky.social! Great team effort with Randall Johnson's lab.

Congratulations! 🎉

ROS transfer at peroxisome-mitochondria contact regulates mitochondrial redox Maintenance of mitochondrial redox homeostasis is of fundamental importance to cellular health. Mitochondria harbor a host of intrinsic antioxidant defenses, but the contribution of extrinsic, nonmito...

www.science.org/doi/10.1126/...

Our new preprint: how photoprotection is spatially coordinated with energy metabolism. Photosystem I is major source of radicals. We report EM structure of PSI in complex with superoxide dismutase SOD: direct link between energy conversion & oxidative stress defence
www.biorxiv.org/content/10.1...

Enhancing autophagy by redox regulation extends lifespan in Drosophila - Nature Communications Redox signalling is emerging as an important regulator of metabolism and physiology, which is dysregulated in ageing and disease. Here, the authors show that redox regulation of a key redox sensitive ...

🎉Excited to share our latest paper, published in Nature Communications!🎉

"Enhancing autophagy by redox regulation extends lifespan in Drosophila"

#redox #metabolism #ageing #Drosophila

@natcomms.nature.com @mrc-lms.bsky.social

doi.org/10.1038/s414...

High-performance genetically-encoded green and red fluorescent biosensors for pyruvate Pyruvate is the end-product of glycolysis and a central metabolite involved in many biochemical pathways. However, a lack of high-performance (i.e., Δ F / F > 10) single fluorescent protein (FP)-base...

New tools! GreenPy and ApplePy are fluorescent biosensors for pyruvate with huge responses (20–40x in vitro) and a range of affinities (10s of μM to mM). These should be game changers for imaging of metabolism! Great work by Shosei Imai @sikmys.bsky.social and team 👏. www.biorxiv.org/content/10.1...

Localization of WDS proteins and fimbrin in three fungal species.

How do cells switch on the actin assembly at endocytic sites? How can this on-switch evolve? Check out our new preprint!
www.biorxiv.org/content/10.1...

1/27 We have a new paper out! Turns out that snowflake yeast have been hiding a secret from us - they've evolved a (very!) crude circulatory system. Not with blood vessels or a heart, but through spontaneous fluid flows powered by their metabolism. 🧪🔬

www.science.org/doi/full/10....

Are you looking for a postdoc?
I will be looking for 2-4 people after the summer to join the lab !!
Do not hesitate to reach out and disseminate !!

Congratulations to Dr Aphrodite Vasilaki and Dr Paraskevi Kritsiligkou @parikki.bsky.social who won the Leopold Flohé Redox Pioneer Young Investigator Award and Catherine Pasquier Award respectively at the 2025 Society for Free Radical Research International meeting
▶️ bit.ly/4ebZ9XN

Spent the day listening to some amazing talks from first and second year PhD students in our Biochemistry, Cell and Systems Biology Department! I loved how confident and knowledgeable our students are! Can’t wait for my students to give their talks next year 🥹

Thanks Ian! Glad you made it to the talk 🙂

I am extremely honoured to have received the 2025 Catherine Pasquier Award from SFRR-E in recognition for Extraordinary Scientific Achievements and Great Future Promise! 🥹 Inspired to follow so many past awardees that are now leaders in the field. Truly thankful for the support. ❤️

Had a great time at the SFRR-I meeting! I listened to some exciting new science, spent time with my friends that live far away and met my fellow @sfrre-ecr.bsky.social in person!❤️ We successfully organised networking events and a sunrise seminar and it is clear we are building a great community 🎉

Outstanding talk by @parikki.bsky.social , recipient of the CATHERINE PASQUIER AWARD LECTURE at SFFR-I Biennial Meeting in Galway @uniofgalway.bsky.social.

Are ROS generated in nanodomains / condensates in cells?

Want to know more, please read her paper
lnkd.in/dq_6Rnaw

@sfrre-ecr.bsky.social

Liverpool's @parikki.bsky.social giving the Catherine Pasquier Award lecture at the SFRRI Biennial Meeting in Galway. Fantastic talk 👏🏻

@liverpooluni.bsky.social @livuni-ismib.bsky.social
@livunihls.bsky.social

Probing condensate microenvironments with a micropeptide killswitch - Nature Targeting a non-natural micropeptide ‘killswitch’ to several biomolecular condensates altered condensate compositions and revealed condensate functions in human cells

www.nature.com/articles/s41... From Denis Hnisz lab

Attending the Careers beyond Gender session this morning! Hozumi Motohashi is an inspiration to us all! ❤️

In Galway for the SFRR-I meeting!With >400 delegates, this week is gonna be epic! If you are an ECR, don’t miss out on the activities the @sfrre-ecr.bsky.social committee is organising! Today join us at 18:30 for a fun meet the professor session and at 19:30 for our annual ECR networking event!