Ah, interesting! If the amplicon data comes from a complex sample, the different ASV proportions could result from non-isolated strains in the microbiome sample, sharing one V4 copy, but not the other and therefore skewing the V4-copy-ratios? then, the least relabund ASV would be the most realistic?
19.09.2025 21:29 โ ๐ 2 ๐ 0 ๐ฌ 1 ๐ 0
Once, I just used vmatchPattern to find the ASV fasta in the isolate fasta. Theoretically, different 16S sequences of the same bug should yield different ASVs. Or do I not get the problem?
19.09.2025 17:09 โ ๐ 1 ๐ 0 ๐ฌ 1 ๐ 0
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22.08.2025 09:29 โ ๐ 2 ๐ 0 ๐ฌ 0 ๐ 0
Hey @enirenberg.bsky.social, my favorite online immunologist!
Do you have an opinion on the immunological surrogates like the Il-10/Il-17 ratio in studies like this. share.google/CvnewT1FoN6D...
I mean apart from the small effect size, do we know this corresponds to meaningful endpoints?
12.07.2025 12:04 โ ๐ 0 ๐ 0 ๐ฌ 0 ๐ 0
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