Troy McDiarmid

Google and Meta search both report that Cape Breton Island has its own time zone 12 minutes ahead of mainland Nova Scotia time because they are both drawing that information from a Beaverton article I wrote in 2024

10.06.2025 00:50 — 👍 6705    🔁 1863    💬 110    📌 219

A multi-kingdom genetic barcoding system for precise clone isolation - Nature Biotechnology A barcoded CRISPR base editing system isolates target clones from complex mammalian, yeast and bacterial populations.

CloneSelect published in Nature Biotechnology
@natbiotech.nature.com. This retrospective clone isolation method using CRISPR base editors is a powerful tool in broad biology. A history of Soh in the Yachie lab. www.nature.com/articles/s41...

21.05.2025 13:56 — 👍 28    🔁 13    💬 3    📌 0

Congrats @nzmyachie.bsky.social and co.! Beautiful work.

21.05.2025 16:59 — 👍 1    🔁 0    💬 1    📌 0

CAGT Poster featuring Keynote, Dr. Calin Plesa, of DropSynth fame!

⏰Abstract deadline for the Cascadia Advanced Genomic Technologies Meeting is April 30!⚠️ We're hoping this will be the first of many, catalyzing collaboration and leveraging our regional strength in advanced genomics technologies! See you there!
de-boer-lab.github.io/CAGT_meeting/

28.04.2025 18:05 — 👍 4    🔁 4    💬 0    📌 0

Massively parallel characterization of transcriptional regulatory elements - Nature Lentivirus-based reporter assays for 680,000 regulatory sequences from three cell lines coupled to machine-learning models lead to insights into the grammar of cis-regulatory elements.

Published in today's issue! OPEN ACCESS article, FREE massive dataset, FREE code, FREE pre-trained AI/ML model. #OpenScience was a mountain of work to make this happen: check it out if you haven't already 😉

13.03.2025 19:32 — 👍 18    🔁 7    💬 0    📌 0

the word irony is written on a white background ALT: the word irony is written on a white background

I have been trying to find the time to move away from the polical hellscape we find ourselves in to finish and share a bluetorial about science.

This helps me remember what this is all about.

Ironically, it is about the treatment of pain.

22.02.2025 10:19 — 👍 332    🔁 91    💬 10    📌 36

I think people both within science and outside science don’t fully appreciate the miracle that were the Covid-19 vaccines. How much courage it took to get the sequence out, how much work had already gone into figuring out the best viral antigen and how best to present it. 1/

23.02.2025 07:23 — 👍 777    🔁 188    💬 9    📌 12

This was a really fun collaboration with @amarderstein.bsky.social where we explored some of the interesting relationships between context-specific non-coding variant effects, disease, and evolution using deep learning models of chromatin accessibility in the brain and heart.

19.02.2025 19:11 — 👍 15    🔁 8    💬 1    📌 0

Beware! AAV libraries prone to length & homology dependent chimerism! Our latest preprint, from the brilliant @jblalanne.bsky.social‬, was a true surprise as related phenomena are textbook for lentivirus (highly relevant to HIV & Perturb-seq) yet to our reading not known for AAV tinyurl.com/5amc562a

17.01.2025 18:17 — 👍 26    🔁 10    💬 2    📌 0

Multiplex generation and single-cell analysis of structural variants in mammalian genomes Studying the functional consequences of structural variants (SVs) in mammalian genomes is challenging because (i) SVs arise much less commonly than single-nucleotide variants or small indels and (ii) ...

Now out in @science.org w/ @jshendure.bsky.social we present 'Genome-shuffle-seq': a method to shuffle mammalian genomes and characterize the impact of structural variants (SVs) with single-cell resolution in one experiment.

www.science.org/doi/10.1126/...

31.01.2025 19:41 — 👍 128    🔁 55    💬 3    📌 4

Important PSA / technical note for any using AAVs for experiments that rely on linked elements/barcodes (e.g. multiplexed reporters, MPRAs, certain single cell CRISPR screens).

In work led by @jblalanne.bsky.social - we saw AAVs unlink/swap barcodes when packaged as a pool - design aware!

14.02.2025 20:42 — 👍 3    🔁 1    💬 0    📌 0

RNA polymerase II at histone genes predicts outcome in human cancer Genome-wide hypertranscription is common in human cancer and predicts poor prognosis. To understand how hypertranscription might drive cancer, we applied our formalin-fixed paraffin-embedded (FFPE)–cl...

This is amazing work, I have no words!! This is inspiring and this is the goal !!!

www.science.org/doi/10.1126/...

14.02.2025 04:40 — 👍 30    🔁 11    💬 0    📌 0