Claudio Alfieri

Amazing work from my neighbour's lab! Many congrats to all the people involved! 👏👏👏👏

Part of something that we have been working on for a while-A 3.1 Å map of a flexibly tethered 38 kDa domain. Hopefully preprinted soon along with a few other structures. #CryoEM

This is super cool and great investigative work! Thanks Oli and co! #smallparticles #cryoEM

High-throughput investigation of cyclin docking interactions reveals the complexity of motif binding determinants - Nature Communications Many protein–protein interactions depend on Short Linear Motifs (SLiMs). In this study, the authors use large-scale binding assays, deep mutational scanning, and structural analysis to map SLiMs recog...

Just published: Our paper on high-throughput analysis of cyclin docking interactions, led by the group of Norman Davey @icr.ac.uk and with some small-complex-plus-peptide #cryoEM by @natalia-mg.bsky.social from my lab. Congratulations, everyone!
www.nature.com/articles/s41...

We’ve uncovered Asgard chromatin structures formed by a Hodarchaeal histone : closed hypernucleosome conserved in archaea and an open form resembling the H3-H4 eukaryotic octasome. Fantastic work by @harshranawat.bsky.social!
www.biorxiv.org/content/10.1...
#Chromatin #Asgard #Archaea #cryoEM

Our integrative approach using #MD and #cryoEM data to construct structural ensembles of #RNA just published on @natcomms.bsky.social doi.org/10.1038/s414... Lead by Elisa Posani, with @magistratolab.bsky.social @bonomimax.bsky.social @pjanos.bsky.social @navtejtoor.bsky.social and Daniel Haack 🎉

A snapshot of the HLp tetramer (purple) wrapping ~60 bp of DNA (green).

Excited to share our new preprint on HLp—a bacterial histone from Leptospira perolatii that forms stable tetramers and wraps ~60 bp of DNA: "DNA Wrapping by a Tetrameric Bacterial Histone" www.biorxiv.org/content/10.1...

@mpi-bio-fml.bsky.social

Many Congrats Sebastian and Team! 🥳🥳🥳

Determining the effects of pseudouridine incorporation on human tRNAs | The EMBO Journal imageimagetRNAs harbour a complex set of modifications which are important for their function. Here, single particle cryo-EM, biophysics, and computational analyses of the effects of pseudouridine inc...

We are very excited about our latest work, which just came out @embojournal.org – www.embopress.org/doi/full/10..... The work @MCB_UJ was driven by Anna Biela and Ting-Yu Lin 林亭妤 in collaboration with @jmbujnicki.bsky.social…a short🧵

New preprint on 3D heterochromatin architecture in human cells! Great collab with @sergiocruzleon.bsky.social & @johannesbetz.bsky.social from @hummerlab.bsky.social, @marinalusic.bsky.social & the Turoňová lab. Many thanks to my supervisor @becklab.bsky.social. bioRxiv: tinyurl.com/3a74uanv 🧵👇

#CryoSPARC v4.7 is out! 🚀 
Featuring a new automatic Micrograph Junk Detector that labels and rejects contaminants, new tools for curating subsets of particles, performance and stability fixes, and more!
Full changelog: cryosparc.com/updates

#cryoEM

🚨New paper🚨Our new study with the lab of Phil Cole uncovers how SIRT6 dynamically adapts structurally and enzymatically to target diverse histone acylations (e.g., H3K27ac) within nucleosomes. @jonmarkert.bsky.social
doi.org/10.1016/j.jb... #Chromatin #SIRT6 #CryoEM

Excited to share our new preprint on co-transcriptional splicing! U1 snRNP is recruited to RNA polymerase II through multiple interaction sites, with elongation factor SPT6 binding directly to the U1 snRNP protein.

www.biorxiv.org/content/10.1...

#cryoEM #transcription #splicing

Many congratulation Suyang and coauthors!

Structural basis for transcription activation through cooperative recruitment of MntR - Nature Communications MntR is a protein that can repress the expression of Mn2+ uptake transporters and activate the expression of efflux proteins. Here, the authors use cryo-EM and other biochemical approaches to establis...

How does MntR function as both repressor and activator with different Mn(II) sensitivity and cooperativity?

Led by the remarkable Prof. Shivani Ahuju (Reed), our long-time collaborator Arthur Glasfeld, and Reed undergraduates!
#MicroSky #ChemSky #cryoEM

www.nature.com/articles/s41...

Sad to know about the passing of Béla Novák. This review from Tyson, Chen and Novak was such a revelation for me as an introduction to control theory in biological systems. A classic that will remain relevant for many years to come. www.sciencedirect.com/science/arti...

📢 We’re looking for a new Team Leader!

@enasequence.bsky.social is recruiting a Team Leader to shape the strategy and direction for the ENA and Biosamples open data resources.

Interested?👇

Today in @nature.com we share our back-to-back stories with Ning Zheng’s lab revealing chemical-genetic convergence between a molecular glue degrader & E3 ligase cancer mutations. 1/5

Making intrabodies from antibodies just got easier! Learn how we made 𝟭𝟵 intrabodies to bind and light up peptides and histone modifications in live cells. And thanks to Academia, all sequences are freely available. (video credit: Yuko Sato @YukoSatoT2) (1/15)
www.biorxiv.org/content/10.1...

The mechanics of a continuous self-assembling surface-layer aids cell division in an archaeon

Tight collaboration with @buzzbaum.bsky.social, Sulfolobus cryo-ET from @idocaspy.bsky.social

www.biorxiv.org/content/10.1...

Cryo-EM captures early intermediate steps in dynein activation by LIS1 Cytoplasmic dynein-1 (dynein) is an essential molecular motor in eukaryotic cells. Dynein primarily exists in an autoinhibited “Phi” state and requires conformational changes to assemble with its cofa...

Today in #journalclub, we looked at the new study from Nguyen et al. showing how Cryo-EM captures early steps in dynein activation by LIS1. They identified a novel ‘Pre-Chi’ intermediate, shedding light on how LIS1 relieves dynein autoinhibition!
www.biorxiv.org/content/10.1...

Postdoctoral Training Fellow in Chelsea | The Institute of Cancer Research View details and apply for this Postdoctoral Training Fellow vacancy in Chelsea . Salary: Dependent on relevant Postdoctoral experience. Future progression is based on annual performan...

At #EMBOinsitu and looking for a motivated postdoc joining an interdisciplinary project in my lab:
jobs.icr.ac.uk/vacancies/11...
the-alfieri-lab.co.uk
#cryoem #cryotem

Our #CryoET paper on ciliary rootlet ultrastructure from mouse retina is now out in @elife.bsky.social! Have a peek with this video or dive deeper at bit.ly/3ZJzhgc. Big thanks to @carter-lab.bsky.social and #teamtomo.

#CryoET #CryoEM #Cilia #Rootlet #Centriole #Research #Science

High-throughput discovery and deep characterization of cyclin-CDK docking motifs Cyclin-CDKs are master regulators of cell division. In addition to directly activating the CDK, the cyclin subunit regulates CDK specificity by binding short peptide “docking” motifs in CDK substrates...

New pre-print! To support a CDK-cyclin docking motif discovery and characterisation project led by Norman Davey and colleagues, @natalia-mg.bsky.social in my lab determined the #cryoEM structures of three peptide-bound CDK2-cyclin A complexes. Check it out!
www.biorxiv.org/content/10.1...

Twenty-seven scientists become EMBO Young Investigators – Press releases – EMBO The group leaders join an international network of nearly 800 life scientists and receive financial support

Thrilled to share that I have been selected as one of this year's new EMBO Young Investigators. I look forward to meeting many of the other Young Investigators in the coming years, and I am most grateful to the members of my laboratory at the ICR who made this possible.
www.embo.org/press-releas...