The EMBO Journal

Thrilled to share our work on the role of Ataxin-3 in lysosome regeneration published in @embojournal.org . Here we show that Atxn3 targets K48-K63 branched ubiquitin chains on damaged lysosomes and mediates restoration of lysosomal function via microautophagy.

www.embopress.org/doi/full/10....

Making sense of oxygen sensing - Thomas Keeley & Peter Ratcliffe describe challenges encountered in measuring and manipulating cell monolayer oxygenation in vitro, and innovative approaches to overcome them
www.embopress.org/doi/full/10....

The small adipocyte-specific transmembrane protein, Nrac, interacts with scavenger receptor CD36 to fine-tune fatty acid uptake and lipid clearance
Siegfried Ussar and collaborators @www.helmholtz-munich.de
www.embopress.org/doi/full/10....

Simultaneous visualization of mRNA-protein interactions and translation status in live cells reveals ZNF598 E3 as limiting factor in resolution of collided ribosomes
Ana De La Cruz, Bin Wu and collaborators
www.embopress.org/doi/full/10....

This cover highlights the article Lysine myristoylation mediates long-term potentiation via membrane enrichment of synaptic plasticity effectors by Benjamin Matthews, Tristan P. Wallis, Frédéric A. Meunier and colleagues. Chemical LTP (cLTP) robustly promotes the formation of new dendritic spines in hippocampal neurons. We detect a marked increase in spine number and an enlargement of spine heads. However, this plasticity is completely blocked by inhibiting either DDHD2 or N-myristoyltransferase (NMT1/2). These findings demonstrate that DDHD2 and myristoylation are key regulators of synaptic plasticity. DIV21 primary mouse hippocampal neuron transfected with cytosolic GFP to visualise dendritic processes and spines. 3D z-stack image acquired 2 hours following chemical LTP showing the number and size of dendritic spines is significantly increased with potentiation. Inhibition of DDHD2 and NMT1/2 abolishes this effect. Scientific image by Sevannah Steeves, University of Queensland

Review in issue 15:
Midbrain organoid models of dopaminergic system
WIP1 affects the mitotic timer
Legionella affects autophagy by SNARE phoshoribosyl-ubiquitination
Dysfunctional mitochondria trap proteins between their membranes
Cover by @meunierlab.bsky.social
www.embopress.org/toc/14602075...

Bacterial IgA proteases –potential therapeutic targets in IgA nephropathy– have distinct specificities for intact or pre-digested antibody molecules - revealed by biochemical and structural work of Beatriz Trastoy and colleagues
www.embopress.org/doi/full/10....

ATXN3-p97 facilitates #lysosomal restoration after damage by deubiquitinating LAMP2 and targeting it for degradation via microautophagy
Hemmo Meyer and coworkers @hemmo-lab.bsky.social
www.embopress.org/doi/full/10....

A new type of #macrophage cellular protrusion: Changyuan Fan et al find that dendrite-like pseudopods form upon massive infection with gram-negative bacteria and enhance bacterial ingestion
www.embopress.org/doi/full/10....

🥁Out on @embojournal.org
👉Optochemical profiling of NMDAR molecular diversity at synaptic and extrasynaptic sites
🔗 doi.org/10.1038/s44318-025-00498-x
👏Congratulations to Antoine Sicard, Pierre Paoletti*, @laetitiamony.bsky.social and all authors and collaborators!

@skamrad.bsky.social @kiranrpatil.bsky.social show xenobiotics impact growth of gut bacteria and their metabolic homeostasis by affecting amine metabolism ➡️ www.embopress.org/doi/full/10....

Ying Yu, Danyang Tian and colleagues show how macrophage-derived #prostaglandins can regulate adipose tissue remodeling: via a signaling cascade initiated by EP3 receptor and impinging on DNA methylation
www.embopress.org/doi/full/10....

The #6mA reader YTHDF3 recruits ssDNA-specific #ALKBH1 demethylase to genomic DNA and allows it to remove 6mA from double-stranded DNA regions
Guang-Rong Yan, Xiao-Feng Gu and coworkers
www.embopress.org/doi/full/10....

Like TDP-43, other ALS-associated RNA-binding proteins MATR3, FUS, and hnRNPA1 also promote UNC13A transcription, with REST downregulation as a common mechanism
Yasuaki Watanabe, Keiko Nakayama et al
www.embopress.org/doi/full/10....

Our new work in @embojournal.org ! 🎉

PARP7 creates an ADP-ribosyl degron on chromatin-bound AR, leading to DTX2-mediated degradation and negative feedback in androgen signaling in prostate cancer.

Full: www.embopress.org/doi/full/10....

#ProstateCancer #PARP7 #AndrogenReceptor #TheEMBOJournal

🚨It’s out @embojournal.org 🚨
Cardiolipin inhibits Caspase-4/11!
Huge thanks to my brilliant supervisors Kate & Pablo, to @s-burgener.bsky.social & Mercedes and all the @inflammasomelab.bsky.social, it was a joy working and laughing with you, and to @brozlab.bsky.social & Si Ming lab.

Deletion of EP3 #prostaglandin receptor in murine macrophages aggravates diet-induced obesity by suppressing SPARC
www.embopress.org/doi/full/10....

The registration deadline is approaching for @fusionconf.bsky.social #Neuroepi25! Book your place by August 7th - great speaker line-up plus @embojournal.org editor Ioannis Papaioannou will attend and present a poster prize.

Details & Registration: bit.ly/3XHb4pm

Scientific image by Constantin Cretu, Tobias Moser, and Julia Preobraschenski

How do ferlins move and engage with membranes? This stunning @embojournal.org journal cover captures two distinct forms of human myoferlin—one floating free, one anchored and locked in action.
👉 Dive into the full breakdown: www.peeref.com/notes/a797b2...

New Research Identifies Disrupted Protein Aggregation as Potential Early Trigger in Alzheimer’s Disease The research, led by Professor Deborah Goberdhan (Nuffield Department of Women’s & Reproductive Health) and Professor Clive Wilson (Department of Physiology, Anatomy and Genetics) and published in…

Just out in @embojournal.org Oxford researchers uncover how Alzheimer’s may begin at the cellular level, with Aβ disrupting protein aggregation in secretory compartments. A new clue in the fight against neurodegeneration.

buff.ly/VkrHhLY

#Alzheimers #OxfordResearch #EMBOJournal #Neuroscience

How does nutrient-responsive #mTORC1 sense the key amino acid #glutamine?
Gianluca Figlia, Aurelio Teleman et al @telemanlab.bsky.social identify a combination of GCN2-dependent processes triggered by secondary depletion of asparagine in glutamine-starved cells
www.embopress.org/doi/full/10....

Human fetal kidney organoids model early human nephrogenesis and Notch-driven cell fate
Benjamin Dekel and coworkers
www.embopress.org/doi/full/10....

The actin-binding/-bundling protein #anillin can also interact with microtubules to crosslink the two types of filaments and slide them along each other
@marileend.bsky.social @gijsjekoenderink.bsky.social and colleagues
www.embopress.org/doi/full/10....

Bryce Paschal and colleagues show that #PARP7 marks the agonist conformation of androgen receptor for #biquitin conjugation by DTX2 E3 with ADP-ribose reader function, resulting in negative feedback of AR-dependent gene expression
www.embopress.org/doi/full/10....

Dissection of Early Replication Control Elements (ERCEs) by Jesse Turner, Laura Hinojosa-Gonzalez et al reveals interacting subcomponents that are bound by master transcription factors and maintain early DNA #replication independent of their roles in transcription
www.embopress.org/doi/full/10....

📣 Paper alert!! Excited to share the 2nd research paper from my time in the @bokelab.bsky.social at @crg.eu, out today in the @embojournal.org!

We asked: "how do human oocytes regulate proteostatic organelles under physiological conditions?"

1/

Amyloid-β disrupts APP-regulated protein aggregation and dissociation from recycling endosomal membranes
Clive Wilson and coworkers
www.embopress.org/doi/full/10....

This cover highlights the article Structural insights into lipid membrane binding by human ferlins by Constantin Cretu, Tobias Moser, Julia Preobraschenski and colleagues. By combining single-particle cryo-electron microscopy, mass spectrometry, and biochemical analyses, this study sheds light on the molecular basis of membrane binding and remodelling by the multi-C2 domain proteins myoferlin and dysferlin. The cover image presents an artistic representation of two resolved structural snapshots of human myoferlin: a more dynamic, lipid-free state (left), which reflects vesicle-anchored conformations, and a compact, ring-like state (right), induced upon membrane binding. Scientific image by Constantin Cretu, Tobias Moser, and Julia Preobraschenski

Highlighted in ISSUE 14:
Influenza filaments mediate spreading
Resource:
m6A landscape during oocyte-to-embryo transition
ATRX protects centromeric cohesion
Fluorescing photoreceptors in fungal zoospore eyes
Cover: Tobias Moser, Julia Preobraschenski and coworkers
www.embopress.org/toc/14602075...

See also nice press release from @crg.eu:
"HUMAN EGGS POWER DOWN TO PROTECT THEMSELVES"
www.crg.eu/en/news/huma...

The proteostatic landscape of healthy human oocytes
Elvan Böke and colleagues @bokelab.bsky.social find that mature human oocytes show reduced activity of lysosomes, proteasomes and mitochondria, highlighting reproductive differences between humans and mouse
www.embopress.org/doi/full/10....

Profiling the proteomes of key organs across the entire mouse lifespan reveals organ-specific as well as systemic alterations associated with aging
Dan Ehninger et al @dzne.science
www.embopress.org/doi/full/10....