Pauli Group (posts by Andi Pauli)

Big congrats to the entire team from the @beckmannlab.bsky.social and @greenlab.bsky.social, in particular first author Vienna!

How does the kinase ZAK sense ribosome collisions? Find out in our latest collaboration with the @greenlab.bsky.social @doubleshuang.bsky.social @Vienna Huso: 1/4
rdcu.be/eRmJl
#ribosome #cryoEM #LMU #JHMI

SAVE THE DATE! Stoked to organize the 2026 Santa Cruz Developmental Biology Meeting with @rashmi-priya.bsky.social, @lowelab.bsky.social, and Shelbi Russell. Come learn about Biomedicine, Biomechanics, and the Biosphere, August 24-28, 2026. Registration dates, etc., coming soon! Please RT

How does messenger RNA (mRNA) get out of the nucleus to become a protein? Eukaryotic mRNA is packaged, exported, and then translated in the cytoplasm. But how do these steps work? And what are open questions? Check out our new review for our take: www.annualreviews.org/content/jour... (1/3)

... and we would love to see you ALL - working with ANY fish model - join us in Vienna for www.ezm2026.org, the biggest fish meeting in 2026! We plan to have a special session 'beyond zebrafish' and would love to include talks representing other fish systems across all sessions. Pls spread the word!

MISO: microfluidic protein isolation enables single-particle cryo-EM structure determination from a single cell colony.

Or from a single dish of HEK cell culture in the case of two membrane proteins.

Out in Nature Methods now! lnkd.in/gpyBSceg

Wonderful collaboration with the Efremov lab.

Proteome-wide in silico screening for human protein-protein interactions Protein-protein interactions (PPIs) drive virtually all biological processes, yet most PPIs have not been identified and even more remain structurally unresolved. We developed a two-step computational...

Thrilled to share that the final piece of my PhD work is now on bioRxiv! biorxiv.org/content/10.1... With support from @nvidia and the @NSF, we used AlphaFold to screen 1.6M+ protein pairs, revealing thousands of potential novel PPIs. All data can be viewed at predictomes.org/hp

Join us at the Vienna BioCenter and @impvienna.bsky.social! Full disclaimer: I’m obviously biased but: mind-blowing facilities (!!!) and a great collaborative research culture make this an ideal place for anyone; especially suited to build up a lab. Plus Vienna is an amazing city to live in 😊

HT-PELSA, a new proteomics tool by EMBL researchers, processes samples 100x faster and works directly with complex crude cell, tissue, and bacterial lysates – developments which could accelerate drug discovery and basic biological research 💊

🔗 www.embl.org/news/science...

New paper alert! Scientists in Clemens Plaschka’s lab at the IMP and @juliusbrennecke.bsky.social's lab at
@imbavienna.bsky.social solved a decade-old puzzle, uncovering how the information molecule mRNA travels from the cell’s nucleus to its periphery. More: bit.ly/4nHcvys

Alternate proteins from the same gene contribute differently to health and rare disease | Whitehead Institute Iain Cheeseman and colleagues reveal the underappreciated role of single genes producing multiple proteins in atypical presentations of rare disease, and present case studies of affected patients thro...

From an accidental discovery of hidden biology to a new framework to understanding and diagnosing rare disease. Thrilled to share the most recent work from our lab and the amazing Jimmy Ly.

wi.mit.edu/news/alterna...

This Halloween, we have a spooky evolutionary story for you.
The brainchild of @delaconcepcionjc.bsky.social, Nick Irwin and our fantastic collaborators is now out in @natplants.nature.com www.nature.com/articles/s41...
Here’s why I love this work — and why I think you’ll enjoy it too. 👇

Very happy to share our latest work extending iterative immunofluorescence to in toto imaging of early zebrafish embryos (3D-4i), integrated with a 3D-dedicated image analysis pipeline! 🐟🔬📊
Huge kudos to Max Hess for this PhD milestone! 💪
@lucaspelkmans.bsky.social shorturl.at/cO0u7
Let's zoom in!

Why curiosity-driven research is so fundamental & important, and requires sufficient funding. It's a corner stone of scientific discovery, which has always been and will always be inherently unpredictable!!

Germ granule localization of nematode Argonaute WAGO-4 ensures fidelity in small RNA loading | The EMBO Journal imageimageGerm granule localization is a conserved feature of many Argonautes (Agos), but its functional significance remains unclear. This work suggests that for the Ago WAGO-4, spatial enrichment in...

Fantastic to see this Stela Jelenic's PhD work (@vbcscitraining.bsky.social PhD student) out in EMBO Journal! www.embopress.org/doi/full/10....

In our latest interview, Kristina Stapornwongkul, the newest group leader at IMBA, shares her journey to joining the institute, why she chose IMBA as the next step in her career, and her vision for her lab exploring how metabolism shapes embryonic development: https://imba.science/49fCrxl

The quest to make babies with lab-grown eggs and sperm Sex cells made from scratch could revolutionize human reproduction. But researchers are struggling to translate promising results in mice into benefits for humans.

Sex cells made from scratch could revolutionize human reproduction, but researchers are struggling to translate promising results in mice into benefits for humans, according to a feature in Nature. 🧪

Claude for Life Sciences Discover how Claude accelerates life sciences research with new scientific connectors, skills, and improved performance for drug discovery and clinical work.

Today @anthropic.com released PubMed integration for Claude. No hallucinations. Just real science, real data. As a beta tester, this has been game changing— a supercharged research tool. Here are 6 prompts that will transform how you search the literature. A 🧵

www.anthropic.com/news/claude-...

Structures from AlphaFold3 - while often impressively good - tend to fail representing the dynamic ensembles accurately. And often parts of the structure are not correct. Adding experimental data, directly in AlphaFold's diffusion step, provides physically realistic protein ensembles. This image shows two cases where AlphaFold3-only structures were largely improved by guiding with experimental data.

📢 New preprint:
Experiment-guided AlphaFold3 resolves accurate protein ensembles.
doi.org/10.1101/2025...

AlphaFold3 is incredible, but has crucial limitations: it typically collapses to a single conformation, ignoring the inherent dynamics of proteins. And it can be wrong. Here's a solution. 🧵👇

Congrats @laurentformery.bsky.social

Often people ask why we chose to work on a certain problems and a simple answer for us in this case - it’s beautiful. If you want to learn the magic of building crystalline « gems » inside the body - see our latest paper at www.biorxiv.org/content/10.1...

Graphical abstract: The Drosophila OSC Genome as a resource for transposon and piRNA biology. The figure illustrates the workflow and key findings. Left: De novo genome assembly using Oxford Nanopore Technologies (ONT) long reads and Hi-C data generates a phased assembly distinguishing unique (blue) and repetitive (orange) sequences. Dot plot comparison between OSC-r1.01 and dm6 reference genomes shows overall synteny with extensive structural variation. Middle: A freely accessible UCSC genome browser session displays multi-omics data tracks including gene models, transposon insertions, chromatin accessibility, transcription, small RNAs, and histone modifications. Right: New insights into flamenco piRNA cluster biology reveal >730 kb transcribed from a single promoter without major splicing. Tethering assays demonstrate long-range silencing effects across the locus, and genome browser tracks show coordinated regulation of piRNA production, transcription, and chromatin state. This resource enables comprehensive studies of transposon regulation and piRNA pathway function in a widely-used Drosophila cell line.

When transposons jump, genomes diverge - even in cultured cells.
I am happy to share our new preprint: a chromosome-scale genome assembly for Drosophila OSC cells, one of the key model systems in the piRNA field, especially for nuclear piRNA biology. 🧬🧵 (1/12)

Congrats Dominik - fantastic to see this out!

Big congrats to all, to @imbmainz.bsky.social on this amazing 'double-catch', and of course to @hohmannulrich.bsky.social & @lorenzoorts.bsky.social for starting their own labs!

To prospective PhD students interested in mechanistic question in gene regulation, check out Laura's & Ulrich's labs!

Very sweet ;-)! Big congrats to the entire team!!

A big round of applause also to all other winners of awards, including former PhD student @mjkellner.bsky.social from our neighbors @imbavienna.bsky.social!

Congratulations to @vdeneke.bsky.social & Andreas Blaha @impvienna.bsky.social for their research on vertebrate fertilization, which was awarded this year's ÖGMBT Life Science Research Award in Austria for basic research!

The Vienna BioCenter PhD Program application deadline is approaching: October 10!

Join one of Europe’s premier life science campuses, home to cutting-edge research across all areas of biology.
Apply and start your scientific journey in Vienna!

training.vbc.ac.at/phd-program/

#PhD #LifeSciences

Are you interested in the mechanisms of mRNA regulation in the early embryo and are looking for a great lab to start your PhD? Then check out this amazing opportunity with my outgoing postdoc @lorenzoorts.bsky.social who will start her own lab - I can highly recommend to apply!

Wonderful news, and big congratulations to my dear friend, colleague, and brilliant scientist @obenaufa.bsky.social for winning this major Award! Super happy for you!

Scientist, advocate and entrepreneur Lucy Shapiro to receive Lasker-Koshland Special Achievement Award Lucy Shapiro entered biology “like a bolt of lightning.” A groundbreaking scientist, mentor, drug developer and advocate for science communication is recognized for a lifetime of achievements.

Congratulations Lucy Shapiro @stanfordmedicine.bsky.social

med.stanford.edu/news/all-new...