Ethan Hughes

Discovery of oligodendrocyte enhancers that regulate Sox10 expression Author summary Oligodendrocytes are a special cell type in brain and spinal cord and form myelin sheaths around axons. Myelin sheaths are lipid-rich structures that are essential for the rapid transmi...

Discovery of oligodendrocyte enhancers that regulate Sox10 expression

journals.plos.org/plosgenetics...

#oligodendrocyte
#myelin

TMEM63A, associated with hypomyelinating leukodystrophies, is an evolutionarily conserved regulator of myelination - PubMed Infantile hypomyelinating leukodystrophy 19 (HLD19) is a rare genetic disorder where patients exhibit reduced myelin in central nervous system (CNS) white matter tracts and present with varied neurolo...

Fantastic new study on the role of TMEM63a in oligodendrocyte-dependent myelination in the CNS from the Swetha Murthy lab: pubmed.ncbi.nlm.nih.gov/40694323/

We had fun assembling images and describing approaches for imaging oligodendrocytes and myelin in vivo, in organotypic slices, and in fixed tissues. This work started in the lab of @jgrutzendler.bsky.social over 10 years ago and continues to keep us busy.
www.nature.com/articles/s44...

Thanks Lindsay! Super excited!

Three-photon microscopy: an emerging technique for deep intravital brain imaging - Nature Reviews Neuroscience Optical microscopy allows neural cells to be studied in the intact brain, but imaging deep neural tissue presents substantial challenges. Prevedel and colleagues outline the principles of three-photon...

A nice Review on 3P neuroimaging in NR Neuro. The images never fail to amaze me. www.nature.com/articles/s41...

Awesome work Stacey! Big congrats to you and your team!

Excitatory-neuron-derived interleukin-34 supports cortical developmental microglia function Neuron-microglia interactions dictate the development of neuronal circuits in the brain. However, the factors that regulate these processes across dev…

Excited to share this new paper from the lab, now out in @cp-immunity.bsky.social! Led by the talented @bendevlin.bsky.social and in collab with @anneschaefer.bsky.social. a brief 🧵

Subcellular proteomics and iPSC modeling uncover reversible mechanisms of axonal pathology in Alzheimer’s disease - Nature Aging Axonal spheroids disrupt neural circuits in Alzheimer’s disease. In this study, using subcellular proximity labeling proteomics in human brain and iPSC modeling, the authors link spheroid formation to...

Excited to share that two studies from my postdoc research were published back to back! In the first, we uncovered the molecular basis of axon spheroids—a key hallmark of Alzheimer's disease—and demonstrated their potential reversibility.
www.nature.com/articles/s43...

Awesome story Jaime, congratulations to you and your team!

Happy to share our latest publication on subcellular myelin proteomics and structural changes in Alzheimer disease www.nature.com/articles/s41...

Three-photon in vivo imaging of neurons and glia in the medial prefrontal cortex with sub-cellular resolution - Communications Biology Three-photon in vivo imaging provides access to medial prefrontal cortex, hippocampus and spinal cord in mice at up to 1.7 mm depth of neurons, dendrites, spines, astrocytes and microglia.

It is out! Read how you achieve 1.7 mm deep 3-photon imaging in prefrontal cortex with dendritic spine resolution over a week.
www.nature.com/articles/s42...

Activity-driven myelin sheath growth is mediated by mGluR5 - Nature Neuroscience Employing pharmacology, genetics and all-optical approaches in zebrafish, Braaker et al. find that neuronal activity influences the growth of myelin sheaths along axons by signaling through metabotrop...

For those who want a distraction and/ or are interested, here's the latest from the group, led by Phil Braaker

www.nature.com/articles/s41...

Congratulations Amy!!!

Our lab's 1st paper, led by @fchrisbennett.bsky.social & @waisenberg.bsky.social. A decade thinking how to directly assess the impact of microglia replacement. Yrs of hard work. This kind of work is needed to expand brain disease care for kids & the vision for safer, more effective cell therapy.

Excited to share this new work led by the talented Mike Dolan @dolan in collaboration with Evan macosko
See below for details and preprint

Fast, accurate, and versatile data analysis platform for the quantification of molecular spatiotemporal signals Optical recording of intricate molecular dynamics is becoming an indispensable technique for biological studies, accelerated by the development of new…

Fun to have contributed a little to this, led by the team of the incomparable Guoqiang Yu.

Suspect that this will prove to be an immensely popular image analysis platform for interrogation of dynamic signallingl!
www.sciencedirect.com/science/arti...

Congratulations Zeeba and team!

Senescent cell reduction does not improve recovery in mice under experimental autoimmune encephalomyelitis (EAE) induced demyelination - Journal of Neuroinflammation Multiple sclerosis (MS) is a chronic inflammatory disease of the central nervous system (CNS) characterized by immune cell-driven demyelination and progressive neurodegeneration. Senescent cells (SCs)...

Happy to see my work published in Journal of Neuroinflammation! Thanks to my amazing mentor @jkhuang.bsky.social and all the co-authors George Melchor, Meghan Bullard, @philgrossphd.bsky.social for their continued help and support. jneuroinflammation.biomedcentral.com/articles/10....

Excited to share our latest paper!

We argue that in MS, the primary immune attack isn’t on myelin or myelinated axons — but on the ion & water homeostasis machinery in astrocyte endfeet.

A short thread on this new perspective

1/11

Interested in #oligodendrocyte metabolism and/or #multiplesclerosis? Our paper is now at Biorxiv! We show that oligos express MCT2, the high affinity monocarboxylate transporter, well known for supplying neurons with astrocyte-derived lactate (astro-neuron lactate shuttle)👇

🥼 Janelia researchers led by @boazmohar.bsky.social @nspruston.bsky.social @rhodamine110.bsky.social & @svoboda314.bsky.social @alleninstitute.bsky.social have developed a new imaging tool that maps brain-wide changes in neuronal connections. 🧠

🔗 www.janelia.org/news/new-ima...

Congrats Lucas and team, cool study!

A single-cell transcriptomic atlas of sensory-dependent gene expression in developing mouse visual cortex Summary: Single-cell RNA sequencing of developing mouse visual cortex uncovers cell type-specific gene programs induced by sensory experience. All cell types mounted transcriptional responses to the e...

journals.biologists.com/dev/article/...

New work from the lab characterizing sensory-dependent transcriptomes in mouse V1 across neuronal and glial cell types. Some fun nuggets in here, and all data publicly available for download and re-analysis. Happy gene hunting!

Enabling global image data sharing in the life sciences - Nature Methods This Perspective provides a roadmap toward globally federated open data resources for bioimaging data.

So happy we finally published this great piece from Antje Keller @antjekeppler.bsky.social and colleagues on the path forward for FAIR bioimage sharing. www.nature.com/articles/s41...

Reversible reduction in brain myelin content upon marathon running - Nature Metabolism Using magnetic resonance imaging on marathon runners, Ramos-Cabrer, Cabrera-Zubizarreta et al. report that the signal detected as a surrogate of myelin content is significantly reduced after exercise,...

Cool paper about a couple of things I love www.nature.com/articles/s42...

Peer review completed within 7 days!
Paying peer reviewers!
Yes, it's possible.

Fast & Fair peer review by @biologyopen.bsky.social

More innovation from @biologists.bsky.social

Details here: www.biorxiv.org/content/10.1...

🚨iGluSnFR4 is finally out!🚨🧪

We present iGluSnFR4f and 4s, a novel pair of genetically-encoded glutamate indicators designed for high-fidelity imaging of synaptic activity in the living brain. ⬇️

www.biorxiv.org/content/10.1...

🎥 Below: iGluSnFR4s detecting minis in cultures w/ TTX

#Neuroscience

Great work Tim and team!!!

I'm pleased to share our most recent study led by Matija Zelic. We profiled human ALS spinal cords to uncover neuroinflammatory microglia and astrocyte states and show a key role for Ripk1 as a contributor to the pathological tissue environment in ALS.

www.cell.com/immunity/ful...

This is awesome! Need immunostaining with two rabbit primary antibodies? No problem, with this approach.

We will be looking into this more closely. Great to have such careful validation and optimization work with these tools, thank you Horrocks’ lab!