Kaitlyn Abe

Our new preprint revealing a new mechanism of mitochondrial disease pathophysiology: the accumulation of toxic complex I intermediates bound to complex III

www.biorxiv.org/content/10.1...

19.06.2025 18:55 — 👍 7    🔁 3    💬 0    📌 0

I am thrilled to share that our manuscript on using LEA proteins to prevent air-water interface damage to fragile samples for cryo-EM is officially out! #CryoEM
doi.org/10.1038/s414...

04.09.2024 18:56 — 👍 11    🔁 3    💬 0    📌 1

Mascot of water bear to the rescue! (drawn by Kaitlyn Abe, lead author)

1/3: Led by @kaitlynabe.bsky.social, we found small LEA proteins from nematodes and tardigrades (water bears!) to be incredibly effective in protecting two test fragile complexes - human DNA polymerase alpha-primase and polycomb repressive complex 2 - from AWI damage during plunge freezing.

09.02.2024 02:40 — 👍 2    🔁 1    💬 1    📌 0

Small LEA proteins as an effective air-water interface protectant for fragile samples during cryo-EM grid plunge freezing Sample loss due to air-water interface (AWI) interactions is a significant challenge during cryo-electron microscopy (cryo-EM) sample grid plunge freezing. We report that small Late Embryogenesis Abundant (LEA) proteins, which naturally bind to AWI, can protect samples from AWI damage during plunge freezing. This protection is demonstrated with two LEA proteins from nematodes and tardigrades, which rescued the cryo-EM structural determination outcome of two fragile multisubunit protein complexes. ### Competing Interest Statement The authors have declared no competing interest.

Exhausted with trying multiple ways to combat the air-water interface (AWI) problem during sample grid plunge freezing? We may have an easy solution for you!
Read our latest preprint on how we use LEA proteins as AWI sample protectants for challenging samples: www.biorxiv.org/content/10.1...

09.02.2024 02:35 — 👍 10    🔁 8    💬 1    📌 1

I am excited to share that my work with @cijilim.bsky.social on protecting fragile samples from air-water-interface damage during cryo-EM plunge freezing is in preprint! www.biorxiv.org/content/10.1...

09.02.2024 03:12 — 👍 10    🔁 5    💬 0    📌 0