Christian Staehr

New on bioRxiv! 🚨
“Pericyte and Endothelial Primary Cilia and Centrioles have Disparate Organization Across the Brain Microvasculature”
This project studying #microvascular cells took an unexpected turn when we discovered that #pericytes have primary #cilia!
🔗 www.biorxiv.org/content/10.1...
🧪 🧵1/8

Thrilled to share that our work is now published in Science! ✨

We found a preference for visual objects in the mouse spatial navigation system where they dynamically refine head-direction coding. In short, objects boost our inner compass! 🧭

www.science.org/doi/10.1126/...

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🚨CALL FOR PAPERS🚨
For our "Integrative physiological considerations of the human vasculature system" #SpecialIssue, which will showcase further insights into vascular haemodynamics and multi-organ diseases, as well as the technology used to monitor and treat cardiovascular disease.
🔗 buff.ly/HN7wlPr

Brain precapillary sphincters modulate myogenic tone in adult and aged mice - GeroScience Brain precapillary sphincters, which are surrounded by contractile pericytes and are located at the junction of penetrating arterioles and first-order capillaries, can increase their diameter by ~ 30%...

🔗 Full Open Access manuscript: link.springer.com/article/10.1...
👇 Curious to hear more about #PrecapillarySphincters?
Check out these threads about the subject:
Characterization: x.com/SorenGrubb/s...
Ultrastructure: x.com/SorenGrubb/s...

Fantastic study shedding new light on regulation of cerebral microcirculation 👇👇👇

Vittani, Lee, Wang, Hiraoka, et al.
KI mouse expressing red fluorescent blood
In situ SpyTag/Catcher attachment of biosensor
ECS SpyCatcher to capture BBB leak
doi.org/10.1101/2025...
We hope this method is useful to a wide range of the biology community.

The cerebral blood flow response to neuroactivation is reduced in cognitively normal men with β-amyloid accumulation - Alzheimer's Research & Therapy Background Accumulation of β-amyloid (Aβ) in the brain is a hallmark of Alzheimer’s Disease (AD). Cerebral deposition of Aβ initiates deteriorating pathways which eventually can lead to AD. However, t...

Once again, someone else says it: "Aβ accumulation affecting brain vessel function could be a very early pathology leading to neurodegenerative disease." doi.org/10.1186/s131... #cerebrovascular

A budget for brain metabolic water production by glucose catabolism during rest, rises in activity and sleep - Fluids and Barriers of the CNS Maintaining brain fluid homeostasis is of critical importance for creating a stable environment conducive to optimal neuronal functioning, nutrient distribution, and waste product removal. In this stu...

Your brain functions not only as a thinking organ but also as a miniature water factory. We here calculate how much water neuronal mitochondria produce by glucose oxidation: 300-400 ml. Of that amount, 100 ml go into brain's interstitial fluid. fluidsbarrierscns.biomedcentral.com/articles/10....

Excited to share our new paper in @acta_physiol: SorCS2—involved in trafficking and synaptic signaling—is important for neurovascular coupling: doi.org/10.1111/apha...

Whisker stim in Sorcs2 KO 🐭 led to ⬇️NVC, ⬇️astrocyte Ca²⁺ signals & disrupted glutamate receptor trafficking.

🇦🇺🦘Just wrapped up 6 incredible weeks of science, collaboration at the University of the Sunshine Coast.
Thanks to Vicki Hinkley for such a productive partnership—I’m excited to continue this collaboration across 🇩🇰 and 🇦🇺. I’m also grateful to the USC CONNECT Programme for the funding 🙏

Also fascinating: cerebral arteries show a stronger myogenic response at lower pressures than extracranial vessels—likely a protective mechanism to prevent a pressure surge to the brain when drinking (🤯 avoided!).

🦒🫀Measuring hemodynamics in awake giraffes sounds like a wild challenge 🤯.
New paper by Christian Aalkjaer et al in @actaphysiologica.bsky.social 🔓: onlinelibrary.wiley.com/doi/full/10....
🦒 While drinking🚰:
⬇️ Heart rate
➡️ Arterial blood pressure
➡️ Carotid blood flow
⬆️ CSF pressure 🧠

Tenure-Track Assistant Professor / Associate Professor in Bioinformatics at Aarhus University, Denmark - Vacancy at Aarhus University Vacancy at Department of Molecular Biology and Genetics - BiRC - Bioinformatics Research Center, Aarhus University

Come join us at the Bioinformatics Research Centre at Aarhus University. Tenure track/associate professor position available with application deadline June 1, 2025. Application details here: international.au.dk/about/profil...

Join our #PhDcourse on #openscience datasets and tools in #Neurophysiology!
We'll dive into #EM, #inVivo #physiology, behavioural analysis tools, and a talk on creating animations from open data! @quorumetrix.bsky.social
📢 Free for NorDoc PhD students, but open to all! Apply now!
lnkd.in/dU_fxqqu

Amazing work out by Hirase Lab 🤩

Oral Glycine supplementation (DT-109 Gly-Gly-Leu) ⏬#Atherosclerosis & lesional #Calcification in ♂️Monkeys

A potential role of Glycine in GSH antioxidant & #Inflammasome in #SmoothMuscleCell

#SignalTransductTargetTher 2025
www.nature.com/articles/s41...

#StainAI: A deep learning tool trained on 8,695 manually labeled cells to classify #microglia into 6 distinct morphotypes (www.nature.com/articles/s42...).
A powerful step toward automated and scalable morphology analysis.
#AI #neuroimmunology #cardiacarrest

#StainAI: A deep learning tool trained on 8,695 manually labeled cells to classify #microglia into 6 distinct morphotypes (www.nature.com/articles/s42...).
A powerful step toward automated and scalable morphology analysis.
#AI #neuroimmunology #cardiacarrest

Vascular Tone of pressurized🐭distal MCA (sham & #IschemicStroke)⏫by BKCa inhibitor

Brain Arterial #EndothelialCell BKca & TRPV4 (immunofluorescence)⏫by acute (0.5~2 h) #Hypoxia or #Stroke across🐁🐀🐖🚶‍♂️

@chrstaehr.bsky.social #ActaPhysiol 2025
onlinelibrary.wiley.com/doi/10.1111/...

Futile reperfusion of ischemic tissue after #stroke increases the risk of death and disabilities. Spatial transcriptomics provides new insights into the vascular alterations that drive this process.

#BBB #cerebrovascular #neurosky #emedsky #medsky
🧪

Changes in endothelial cell large conductance calcium-activated potassium channels (BKCa) after #hypoxia may explain the altered myogenic tone which impacts blood flow following ischemic #stroke.

#ionchannels #vascularbiology #physiology #cerebrovascular #endothelium #neurosky #emedsky #medsky

#SpatialTranscriptomics #Proteomics acute stage of #IschemicStroke (🐭tMCAO 24h)

Molecular drivers of mural cell hyperconstriction & BBB disintegration in post-stroke futile reperfusion?
A dual non-neuronal role of Calcineurin?

@chrstaehr.bsky.social #Stroke 2025
www.ahajournals.org/doi/10.1161/...

Thank you, Anna 🙏

🚨 Publication alert!🚨 Our new study (onlinelibrary.wiley.com/doi/full/10....) in Acta Physiologica shows that #stroke/#hypoxia induces the expression of BK-Ca channels in the #cerebrovascular #endothelium across species 🧔🏻‍♂️🐭🐷🐀. These channels, absent in normoxia, regulate post-stroke arterial tone.

Our review did not only focus on cardiac arrest but acute treatment of hyperkalemia in general. Anyways, thanks for sharing 👍 an interesting study indeed.

Hello All Scientists! We are the Microcirculatory Society and we are excited to join the community here at BlueSky! Follow us for fun updates happening in the microvascular community and the amazing science being done by our scientists!
#microcirculation #science

Is this real? I published it again on Brain @brain1878.bsky.social 😆

Truly appreciate the leadership of @rrust.bsky.social on this great project (>300 refs!)🫡👇

The blood–brain barrier: a help & a hindrance

#Aging #Stroke #Neurodegeneration

Crossing #BBB 😎

academic.oup.com/brain/advanc...

New review from our lab in
@brain1878.bsky.social

We explore how molecular blood-brain barrier (BBB) changes in aging, neurodegeneration and stroke affect disease progression, delivery of therapeutics, and much more!

academic.oup.com/brain/advanc...

#Pericyte #PulmonaryHypertension
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A subpopulation of Higd1b+ lung capillary pericytes
👉Migrate to distal arteriole
👉Upregulate Vimentin
👉Acquire #SmoothMuscleCell identity (α-SMA, circumferential wrapping)
in🐭Hypoxic PH

#SciArt
Ke Yuan lab @embojournal.org 2025
www.embopress.org/doi/full/10....

This is a groundbreaking study for pericyte fans. We discovered the unique pericyte marker Higd1b which only labels pericytes without any off-target effect from other mural cells.