Michael Aregger

Single-cell exon deletion profiling reveals splicing events that shape gene expression and cell state dynamics Nature Communications - Alternative splicing generates diverse protein isoforms, yet the functions of most exons remain unknown. Here, the authors introduce scCHyMErA-Seq, a scalable single-cell...

We’re excited to introduce scCHyMErA-Seq, a scalable #CRISPR-based #exon-deletion screening platform with #single-cell transcriptomic readouts, enabling high-throughput functional analysis of #alternative #splicing. In collaboration with the Gonatopoulos-Pournatzis lab. @natcomms.nature.com

Check out the latest paper from the Gonatopoulos-Pournatzis lab using CRASP-seq, an RNA-coupled #CRISPR screening platform, to uncover novel #splicing regulators.

The interplay of DNA repair context with target sequence predictably biases Cas9-generated mutations - Nature Communications Here the authors measure how the absence of certain DNA repair genes change the frequencies of unique mutational outcomes generated by Cas9 DSBs at synthetic target sequences in mouse embryonic stem c...

www.nature.com/articles/s41... A collaborative project incepted by Michael Kosicki, and executed by Ananth Pallaseni (both Ph.D students at @sangerinstitute.bsky.social at the time) and Elin-Madli Peets.