Joe Brancale

Continuing to crush and such legendary and tough course!

Congrats to your dad and MAHA! How can you not be proud of good people doing good work

That’s a huge race with a great field this year! Congrats she should be SOO proud (as should you obv)! Great XC career ahead!

On the left - western blot of B16F10 cells wt and KO for CDK8. Our in house produced antibodies give a lot of unspecific bands. On the right same probes with antibodies preincubated with fixed CDK8 KO cells - there is a specific band and faint unspecific bands, which can be probably eliminated with increase of amount of KO cells.

Neat trick if you polycolonal ab's suck. Incubate them with fixed cells with a KO of your protein of interest, then spin. Protocol here: www.med.upenn.edu/markslab/ass...
I was amazed how well it worked on first try (I'm sure that I can completely eliminate unspecific bands)
#WesternBlot #cellsky

So cool! Looking at skin cells to understand psychosis

Check out our new RAEFISH spatial transcriptomics technology published in Cell! This work represents the first time that transcripts from more than 20,000 genes were directly imaged in situ with any technology, and the first time numerous gRNAs were directly probed in an image-based CRISPR screen.

Ever since my early Evo-Devo days I have loved non-traditional models! Hope to get back to it some day, but in the meantime love to see scientists pioneering this type of work

Revisiting a Breathtaking Publication in the History of Molecular Biology

The last publication (he says!) from Rich Losick, my PhD advisor.

Historical in nature it focuses on a seminal paper in molecular biology, the study revealing the genetic code to be triplet in nature. Come for the science, appreciate the clear prose!

www.sciencedirect.com/science/arti...

24 years ago today, I fell in love in NYC. In the shadow of Ground Zero & the horrors of that day.

We have been married for 22 years now. And we have two fabulous children.

I think of this whenever I worry about the darkness.

There can be beauty, even when the worst of humanity is on display.

Please RP.
We are thrilled to announce that our lab’s first preprint is out!
”Whole-genome single-cell multimodal history tracing to reveal cell identity transition”

We report HisTrac-seq, a multiomic single-cell molecular recording platform.
www.biorxiv.org/content/10.1...

Images of cells showing protein mislocalization caused by a Noonan syndrome mutation, with the idea to screen drugs to find any that can reverse the unhealthy mislocalization

Have $10M and want to cure some diseases?

It costs ~$1B to invent a new medicine…but $10M buys you Project Encore, to see if ANY existing drug might be repurposed for ~100 diseases that have no treatments.

Contact me - be a hero for desperate patients!
docs.google.com/document/d/1...

A new tragedy for the Endocrine community An outstanding scientist leaves us too soon

I’m glad to know I’m not the only one who constantly googles “tocris calculator”

Funny cause it’s true 🤦🏽‍♂️🤦🏽‍♂️

Toward single-molecule protein sequencing using nanopores Nature Biotechnology - Maglia and colleagues discuss advances in nanopore technology en route to single-molecule protein sequencing

Nanopore technology might be able to sequence proteins soon! Read about the road ahead in this exciting Perspective rdcu.be/edWPi in @naturebiotech.bsky.social

Best feeling!

Metabolic rewiring in skin epidermis drives tolerance to oncogenic mutations - Nature Cell Biology Hemalatha et al. track the endogenous redox ratio (NAD(P)H/FAD) with live imaging in the mouse skin to study the interface of wild-type stem cells with oncogenic mutant cells. They find that maintaini...

💫NEW: @valentatormenta.bsky.social, Perry & co track the endogenous redox ratio (NAD(P)H/FAD) with live imaging in the mouse skin and find that maintaining a robust redox ratio is key to clonal prevalence.
www.nature.com/articles/s41...

Love research applying newer microscopy techniques to liver biology!! Congrats @hannahhrncir.bsky.social and @graczlab.bsky.social

The MAHA Commission Launches A new executive order, red flags and wet blankets

We have a new EO launching the MAHA Commission. There's a lot of enthusiasm in nutrition spaces that MAHA will finally bring a spotlight to food, but I have a number of concerns - from un-serious descriptions of the problems we face to chemophobic dogwhistles
kcklatt.substack.com/p/the-maha-c...

This is a screenshot of the webpage for the FDA Approved Risk Evaluation and Mitigation Strategies (REMS) website that now reads, "The FDA's REMS@FDA website is temporarily unavailable. Approved REMS remain in effect, even while the website is unavailable. Thank you for your patience."

We're seeing continued censoring from the administration of scientific and clinical information including this crucial webpage from FDA of "Approved Risk Evaluation and Mitigation Strategies (REMS)" which serve as critical programs for drug safety. No explanation, no notice. #MedSky

'Conjunctival Icterus,' Not 'Scleral Icterus' To the Editor.—  The expression "scleral icterus" generally used by physicians is grossly incorrect. For decades, physical diagnosis primers have cued medical students to look for "scleral icterus...

The correct term is conjunctival icterus.

The expression "scleral icterus" was previously and incorrectly perpetuated. See this scathing remark in @jama.com by an ophthalmologist.

jamanetwork.com/journals/jam...

#Liversky #GiSky #Medsky @cwruim.bsky.social

A poem for today. www.poetryfoundation.org/poems/89897/...

Bile acid synthesis impedes tumor-specific T cell responses during liver cancer The metabolic landscape of cancer greatly influences antitumor immunity, yet it remains unclear how organ-specific metabolites in the tumor microenvironment influence immunosurveillance. We found that...

Interested in how T cells are regulated in a tissue-specific manner? Karthik Varanasi’s exciting work shows Bile Acid synthesis impedes tumor-specific T cell responses during liver cancer @science.org Huge THANKS to all the amazing collaborators + @salkinstitute, SBP
www.science.org/doi/10.1126/...

Impressive paper out in @science.org by the Kaech lab investigating the role of bile acids in mediating T-cell response in HCC. #Liversky #Gastrosky www.science.org/doi/full/10....

This. This is what good science, medicine and public health can achieve!

GRETA graphical abstract

We present Gene Regulatory nETwork Analsyis (GRETA), a framework to infer, compare and evaluate gene regulatory networks #GRNs. With it, we have benchmarked multimodal and unimodal GRN inference methods. Check the results here 👇
Paper: doi.org/10.1101/2024.12.20.629764
Code: github.com/saezlab/greta

A Diagnostic Blind Spot: Deep intronic SVA_E Insertion identified as the most Common Pathogenic Variant Associated with Canavan Disease Canavan disease (CD) is a neurodegenerative disorder caused by biallelic disease-causing variants in the ASPA gene. Here, we utilized long-read sequencing (LRS) to investigate eight individuals clinic...

Been looking forward to sharing this for a long time! Great collaboration with folks from across the world in which we identified a SVA_E insertion missed by standard clinical testing in individuals with Canavan disease, an early onset neurodegenerative disease.

www.medrxiv.org/content/10.1...

I’ve been out of the microbiome field field for a few years now but every time I see amazing work like this I’m impressed at how far we have come! Congrats @gangfang.bsky.social and @yufan01.bsky.social

Today we report that an engineered skin bacterium, swabbed gently on the head of a mouse, can unleash a potent antibody response against a pathogen. Could lead to topical vaccines that are applied in a cream. @djenetbousbaine.bsky.social led the charge... @natureportfolio.bsky.social 1/55

eLife will send WOS a partial feed of "solid" articles. This decision will be applauded by some supporters but lamented by others as de facto re-introduction of an accept/reject decision... elifesciences.org/inside-elife...