@thelastegor.bsky.social
PostDoc in Molodtsov Lab The Francis Crick Institute, London Optical tweezers, single-molecule fluorescence microscopy SMC complexes, DNA repair, CRISPR
How do SMC complexes load onto DNA to get ready for loop extrusion?
@roisnehamelinf.bsky.social & co discovered that Wadjet, an SMC complex involved in bacterial DNA immunity, performs some impressive molecular gymnastics 🤸♂️🤸♂️🤸♂️.
Check out the new paper: www.cell.com/molecular-ce...
A diverse team of Crick researchers has come together to make some surprising new insights into how copies of DNA are held together by the ring-shaped protein complex cohesin during the replication process.
www.crick.ac.uk/news/2025-09...
Our Wadjet-II paper by @roisnehamelinf.bsky.social is out in polished form: www.cell.com/structure/fu...
With a great preview by @kevincorbett.bsky.social & @amardeeep.bsky.social highlighting the potential of Wadjets:
Wadjet—Keeping a watchful eye on circular DNA.
www.cell.com/structure/fu...
Our paper is now out in Molecular Cell!
Check the thread in this former post:
www.cell.com/molecular-ce...
I'm very pleased to share this work, which discovers a new material property of DNA—the bending friction— in essence how "sticky or viscous" DNA is as it bends & loops due to all the complex molecular interactions within itself & water
@imperialcollegeldn.bsky.social
@crick.ac.uk
@ucl.ac.uk
⚛️🧪
A new material constant of DNA is the major determinant of looping dynamics https://www.biorxiv.org/content/10.1101/2025.07.10.664086v1
16.07.2025 21:48 — 👍 5 🔁 2 💬 0 📌 0
We found a new asymmetry in the large-scale chromosome structure: sister chromatids are systematically shifted by hundreds of kb in the 5′→3′ direction of their inherited strands! The work was led by Flavia Corsi, in close collaboration with the Daniel Gerlich lab.
www.biorxiv.org/content/10.1...
1/
I’m excited to announce that my paper describing non-canonical mitotic mechanisms in the early mouse embryo is out in @science.org ! (link at end of 🧵)
23.05.2025 19:56 — 👍 197 🔁 46 💬 8 📌 1
Earnshaw, Goloborodko, Dekker & Mirny labs are excited to present our latest work, "Rules of engagement for condensins and cohesins guide mitotic chromosome formation" - now accepted!!
www.science.org/doi/10.1126/...
A short clip describing the key results:
www.youtube.com/watch?v=pmvO...
Very pleased to share our new story on SMC complexes, a great team effort from @roisnehamelinf.bsky.social, @taschner.bsky.social in the GruberLab and James of @dnatopologylab.bsky.social We find that SMC hinge domains are ssDNA gates to facilitate obstacle bypass during DNA loop extrusion!
18.03.2025 08:03 — 👍 14 🔁 5 💬 2 📌 0
Side view of the structure of a monomer of type II Wadjet.
Happy to share the beautiful structure of a Wadjet SMC complex by @roisnehamelinf.bsky.social, with support from the DCI and @drhonsworth.bsky.social ! This is a type II Wadjet with unique characteristics including a tandem KITE subunit, distinct coiled coil architecture and a deviant hinge.
11.03.2025 09:22 — 👍 96 🔁 33 💬 1 📌 2A new PhD position is available in my lab to explore DNA replication mechanisms using single-molecule approaches. Apply by 19th of March:
www.crick.ac.uk/careers-stud...
For anyone that was too busy at the end of last year to think about PhDs or are still looking for the right project/place, I have a project available in the second round of the Yorkshire Biosciences DTP scheme looking at the dynamics of human condensin:
www.findaphd.com/phds/project...
Congratulations to @mmlab.bsky.social and team, who using the C-Trap, were able to grow microtubules from beads in solution and show that HSET (kinesin-14 family) with microtubule tip-tracking protein can harness forces generated by growing microtubule tips: www.nature.com/articles/s41...
12.02.2025 15:43 — 👍 4 🔁 1 💬 0 📌 0Why do different DNA molecules occupy different places in the nucleus? What drives chromatin compartmentalisation? Read this amazing paper to find out www.science.org/doi/10.1126/...
Many congratulations to all the authors of this brilliant work! @rkoszul.bsky.social
Exciting news 📃🧬🔬
Today we report on the dynamics of a fascinating class of molecular motors, so-called SMC proteins in @cp-cell.bsky.social
doi.org/10.1016/j.ce...
Come and do your PhD with us @TheCrick and @UCL_IPLS starting October 2025. Apply here: www.ucl.ac.uk/epsrc-doctor...
20.12.2024 14:29 — 👍 2 🔁 1 💬 0 📌 0
We measured forces generated in bipolar spindles replacing spindle poles with optical traps!
Read more here: www.nature.com/articles/s41...
Please share: Still plenty of time to apply for PhD projects in my lab using single-molecule methods to look at disordered regions and condensin complexes!
Contact me at e.cutts@sheffield.ac.uk
For details on how to apply: www.findaphd.com/phds/project...
👀SMC 🛵 on 🧬 induce 🥨 at each➰
www.science.org/doi/10.1126/...
by @ceesdekker.bsky.social @romanbarth.bsky.social et al.
Graphical abstract showing the loop extrusion mechanism of the Wadjet complex, which selectively cleaves circular DNA while leaving linear DNA unaffected.
Happy to see our research on SMC Wadjet published in Molecular Cell. We showed DNA loop extrusion (LE) by a bacterial SMC, confirming LE is conserved across all life forms. Wadjet's LE activity is crucial for recognizing and restricting foreign plasmids, serving as a defense mechanism. 🧬🔬🧪
02.12.2024 16:59 — 👍 77 🔁 20 💬 5 📌 4First attempt in a (loosely defined) Single-Molecule Biophysics starter pack. Please share and (self-) nominate. go.bsky.app/CteFZM5
13.11.2024 08:23 — 👍 108 🔁 61 💬 66 📌 3Hi Johannes! Could you please add me as well?
28.11.2024 22:49 — 👍 1 🔁 0 💬 1 📌 0
