Caitie McCafferty

📢 Excited to share our new study in Nature Communications!
We show that the A-C linker safeguards centriole architecture & controls duplication. A big congratulations to Lorène for this beautiful discovery, which represents 4 years of her thesis work.
www.nature.com/articles/s41...

Primary cilia and BBS4 are required for postnatal pituitary development Primary cilia orchestrate several signaling pathways, and their disruption results in pleiotropic disorders called ciliopathies. Bardet Beidl syndrome (BBS), one such ciliopathy, provides insights int...

‼️🚨Preprint alert! 🚨‼️
Excited to have some of my first works as a postdoc, and first corresponding author (!!), on biorxiv! This was a fun side quest of a project marrying a few things I deeply love, development, cilia, and hormones in the pituitary! Happy reading!

www.biorxiv.org/content/10.1...

Such a pleasure to be co-organising the #Latsis Symposium this year with Guillermina !

Thanks to all the speakers & sponsors.

And all our teams members who helped make this a reality.

It just felt right.

#Science #WildCells #ProtistsOnSky #Cell #Biology #Biophysics #Behaviour

Wow! Really nice collaborative work in this piece on RSG1 in human ciliopathy and transition zone assembly

Woo! Very excited to share our collaborative work on the centriole's Luminal Distal Ring ⭕. This project started 13 years ago with @hugovdhoek.bsky.social & @stearnslab.bsky.social, and then beautifully came together with @computingcaitie.bsky.social & @centriolelab.bsky.social.
#TeamTomo #UExM 🧪🧶🧬🔬

Omaya Dudin Interview with Omaya Dudin, who uses Ichthyosporea as models to study how and why unicellular organisms evolved multicellularity at the University of Geneva.

90% of you probably don’t need to read this.
But maybe some of you are curious & 10% will feel seen. Or a little less alone. This isn’t about seeking sympathy.
It’s about sharing something hard to say out loud;
partly to heal, partly in case someone needs to hear it too. (1/3)
tinyurl.com/DudinO

We’re kicking off the DinoSphere Online Seminar Series! Join us for our first session with Karel Mockaer (Heidelberg) & Yong Heng Phua (OIST)

📅 1 July 9AM CEST
🔗 tinyurl.com/4mjaverj

Spread the word!
@protistwtmostest.bsky.social @ehehenberger.bsky.social @chandnibhickta.bsky.social&Norico Yamada

Share with your network, group members, colleagues & friends.

We are extending the deadline till the 10th of June.

This is your last chance of joining us this summer.

#Science #ProtistsOnSky #Cell #Biology #Microscopy

I am joining and posting here to publicly shame myself if I don’t have a paper on biorxiv by the end of the summer 🤠

Awesome!! Can’t wait to look into some of my favorite ciliary complexes :)

hu.MAP3.0: atlas of human protein complexes by integration of >25,000 proteomic experiments | Molecular Systems Biology imageimagehu.MAP3.0 integrates mass spectrometry experiments to identify human protein complexes. Using this resource, this study characterizes covariation of complexes, identifies mutually exclusive ...

Very excited that our work describing hu.MAP3.0 is published in @molsystbiol.org. Here we use machine learning to integrate >25k mass spectrometry experiments to place ~70% of human proteins into 15k protein complexes.

www.embopress.org/doi/full/10....

Great dinner with our cryoET friends from Basel! @sofie-dot-rec.bsky.social @computingcaitie.bsky.social @cellarchlab.com @jacky and Mohamad Harastani

🌱 Excited to share that I will start my lab at EPFL, CH this June supported by a @snsf.ch Starting Grant! ramakrishna.ch

We are interested in plant mineral nutrition and will deep-dive into the fine-tuned mechanisms employed by plants to adapt to abiotic stresses such as salinity.

#newPI #plantsci

What a fantastic day of science at FCBIS! @friedlab.bsky.social and I had a blast co-organizing this exciting dive into the chemistry-biology interface—great conversations, amazing energy, and a standout keynote by
@edwardmarcotte.bsky.social. Thanks to all who joined us!

Omg looks amazing!!

I'm super happy that our story is now published!
📖 www.science.org/doi/10.1126/...
But what changed compared to the original preprint?

Also, I feel i should post Movie 1 🎥, that inspired the cover. Back when I did the original bluesky thread, movies were not available.

The final version of our work on the in-cell architecture of the mitochondrial respiratory chain is now online in @science.org 🎉
You can find the full story here www.science.org/doi/10.1126/...
@verenaresch.bsky.social did a wonderful job on the cover and animation, she really brought it to life 👩‍🎨

Choanoflagellates and other holozoan relatives of animals arranged in the shape of the Eiffel tower. Drawing by Eva Pillai.

Excited to announce the 10th "Choanoflagellates & Friends Workshop" over May 20-22 in our own Institut Pasteur in Paris: 2025choanocon.wordpress.com Registrations open til March 15, abstract til March 5. Pre-conference event for students/postdocs on 19/05. Plenty of slots for selected talks/posters!

We (@cellarchlab.com peeps, @manondemulder.bsky.social @phaips.vd.st @fannyleblanc.bsky.social @computingcaitie.bsky.social and Ricardo) had a blast at the EMBO in situ meeting in Heidelberg! Meeting old friends and making new ones! Lots of cool science and new ideas to try 👨🏻‍🔬🔬

Excited to share our ✨preprint✨ from my work in Ted Wensel's lab! We present novel insights into the photoreceptor-specific roles of CEP290. 🧵👇 Thanks for the helpful participation from everyone involved, especially @mrobichaux.bsky.social and @agostolab.bsky.social! www.biorxiv.org/content/10.1...

Elemental cryo-imaging reveals SOS1-dependent vacuolar sodium accumulation - Nature This study demonstrates that cryo nanoscale secondary ion mass spectrometry (CryoNanoSIMS) enables direct multi-elemental imaging at subcellular resolution of macro- and micronutrients or trace elemen...

Most plants can`t handle salt, but have evolved strategies to survive in it. How do they do it?🌱🧪

Excited to share some of our first insights using a new cryoelemental imaging technique to answer these questions at the subcellular scale!(1/8)
www.nature.com/articles/s41... @nature.com #PlantScience

iAPEX: Improved APEX-based proximity labeling for subcellular proteomics using an enzymatic reaction cascade Ascorbate peroxidase (APEX) is a versatile labeling enzyme used for live-cell proteomics at high spatial and temporal resolution. However, toxicity of its substrate hydrogen peroxide and background la...

Proud to share our work spear-headed by PhD student Tommy Sroka with major contributions by #xenopus expert Kerstin Feistel.

Our iAPEX #ProximityLabeling method for #MassSpec based subcellular #proteomics works by locally generating H2O2 using a D-amino acid oxidase that activates APEX2 in situ.

Towards community-driven visual proteomics! Excited to finally share this large-scale curated & annotated dataset of 1829 high-quality #cryoET tomograms of the little green alga that just keeps giving— Chlamydomonas! 🧪🧶🧬🌾🌊🌍

Preprint📜: www.biorxiv.org/content/10.1...

A short thread🧵👇

Best way to start 2025! Thrilled to share our Nature paper about the structures of axonemal components from sperm flagella and from epithelial cilia of the oviduct and brain ventricles. Great collaboration with Tzviya Zeev-Ben-Mordehai and @alanbrownhms.bsky.social

www.nature.com/articles/s41...

Ancient eukaryotic protein interactions illuminate modern genetic traits and disorders - PubMed All eukaryotes share a common ancestor from roughly 1.5 - 1.8 billion years ago, a single-celled, swimming microbe known as LECA, the Last Eukaryotic Common Ancestor. Nearly half of the genes in moder...

I think I follow lab topics more than specific papers, and easily my top (def top 3) thing [still] going on in 2024 in proteomics land is @edwardmarcotte.bsky.social et al.

pubmed.ncbi.nlm.nih.gov/38853926/

www.biorxiv.org/content/10.1...

Towards community-driven visual proteomics with large-scale cryo-electron tomography of Chlamydomonas reinhardtii In situ cryo-electron tomography (cryo-ET) has emerged as the method of choice to investigate structures of biomolecules in their native context. However, challenges remain in the efficient production...

More than 1'800 tomograms available for the community. Towards visual proteomics using our favorite organism Chlamy!

Check out the preprint: www.biorxiv.org/content/10.1...

Find it on EMPIAR (11830) and fully annotated on the CZI data portal: cryoetdataportal.czscience.com

#TeamTomo

😂

Yay I get to stay!

We hope that this data will serve as a valuable resource to aid in the interpretation of ciliary studies and I am excited to share that there are more interactomes for the community on the way 😉

This work provides functional insights into several poorly-defined ciliopathy proteins, which we were able to test in vertebrate multiciliated cells showing functional interactions