Sebastian S. Cocioba

Oh those are a joke. I mean like actual agri tech aka GM crops and consumer facing ag biotech. Anything beyond seed, sun, and soil is a grift in most cases. Very few examples of sensible improvement in cost but never scale.

Majority of crop grown in the US are genetically modified. Over 90% of all corn acreage is GM. There is no such thing as traditional agriculture. We can't negotiate with locusts and blight. We can only fight back with science.

Is it that agricultural biotech doesn't scale fast enough or are some investors not actually into disrupting anything meaningful?

Edit: Not albino, just pigmentless*

My presumed albino mutant stands out in a forest of pigmented stems. Greatly looking forward to this guy's blooms.

Cefotaxime 500mg/L but will also test meropenem soon

They swarm around the wound site and make a biofilm. The cells express RFP as a tracer to compare against any contamination that may arise. RFP stays inside cells.

Yeah a little too long. Gonna see if they can still be salvaged. Looks like 48hrs is the sweet spot.

Another round of Alice petunia transformations. I let them bake for a full 72hrs before replate. Note the link halos around the explants. That's agrobacterium carrying the RUBY plasmid, along with a bacterial RFP acting as tracer.

Yay, the first of my snapdragon crossed took! Waitin on the other guys now...

One-shot design of functional protein binders with BindCraft - Nature BindCraft, an open-source, automated pipeline for de novo protein binder design with experimental success rates of 10–100%, leverages AlphaFold2 weights to generate binders with nanomolar affinity without the need for high-throughput screening.

BindCraft: an AlphaFold2-powered de novo protein binder design tool 🧬✨
Tested in some bacterial systems too! Designs so far are short peptides or mini-domains. Looking forward to long/full protein design ahead! 🧪 #microsky
www.nature.com/articles/s41...

Thats a beautiful cab

One of my favorite cabs. Only $22.

lol growth in increasing salinity for a client bug. From meh to "i hate it here"

Im trying this thing where I use floral dip buffer (with silwet added) as the co-culture buffer for when exposing leaf disks to agrobacterium. My thinking is it helps bring agro closer to wounded tissue by breaking surface tension on hairly leaf bits. Gonna try a few times n see.

How very predictable. All that talk about disruption and technoutopia, only to be spineless against a government who thinks themselves a dictatorship. Wildly un-American.

Silicon valley soft af.

Last bit of work before weekend. Starting another round of Alice petunia transformations. Sterilized 20mins in 2000ppm NaDCC bleach, 2x distilled water rinse, punch 6mm leaf disks, soak in angry agro culture 10mins dark, incubate abaxially on MS with hormones in darkness 72hrs.

good security detail, tbh

Amazing

Evolution be like:

Not really. Whatever is in my pipes grows slowly and has not been an issue. I could swap for black pots but i dont want to disturb roots to check water level or design a sensing system with redundancy. Algae also oxygenate the stagnant water so win win :)

He keeps the actual pests in check so I respect his hustle. I give em a cap full of water; gotta the ambush predators hydrated.

Grow, my lovelies.

Found another weirdo! Two sets of branches from the apex.

More crossing today. I am become plant breeder.

Weird amount of bugs this year. Tons of beetles and lacewings too. Look how well fed this guy is, lol. Go get em, you terrifyingly efficient hunting machine.

Do y'all like this bloom style? I like the clamshell shape but its quite different from conventional snapdragons.

These guys refuse to open; I respect their defiance. You do you, bruv.

Advancing to M2.

Does anyone know if nanopores can read cyclobutane bonded thymines? The kind that UVC causes. Would love to send some in-vitro zapped plasmids to @plasmidsaurus.bsky.social and see if it can detect the TT, TC, and rarer CC dimers. Could be a fun way to mutagenize if transformed in darkness.