Sofía C. Fajardo

CHIP protects lysosomes from CLN4 mutant-induced membrane damage Nature Cell Biology - Lee et al. use an aggregation-prone CLN4 mutant that causes lysosomal damage in neurons and show that in non-neurons, the ubiquitin ligase CHIP prevents CLN4-dependent...

Happy to share an exciting study from Yihong Ye’s lab at NIH, with a minor contribution from our lab: ceroid lipofuscinosis-4 (CLN4)-linked DNAJC5 mutations cause lysosomal damage as a driver of neurodegeneration in iPSC-derived neurons. CHIP safeguards lysosomes via microautophagy 👉 rdcu.be/eChof

Visualizing sub-organellar lipid distribution using correlative light and electron microscopy Lipids and proteins compartmentalize biological membranes into nanoscale domains which are crucial for signaling, intracellular trafficking and many other cellular processes. Studying nanodomain funct...

Ooh nice preprint! Studying membrane nanodomains needs nanoscale lipid localization - current methods fall short. 'Lipid-CLEM', correlative light & EM with click chemistry to map lipids. In endosomes, it shows sphingomyelin’s differential partitioning beautifully.
www.biorxiv.org/content/10.1...

Don't miss the 2025 Gordon Conference on "Lysosomal Diseases" in California (March 2 - 7, 2025)! t.co/vwcNoDwvHg

The 2025 EMBL Annual Poster, listing all of the courses and conferences taking place in 2025 at EMBL

Hi Bluesky community! 👋

Are you looking forward to 2025 as much as we are? 👀

We just updated our 2025 Annual Poster to include even more events for you to join, so go and have a look!

➡️ s.embl.org/poster-bl

#EMBLEvents #molecularbiology #lifesciencetraining

Probes 101 – How to choose a fluorescent probe for your imaging experiment - FocalPlane Probes 101 – How to choose a fluorescent probe for your imaging experiment - Tools

📢Now up on FocalPlane: Probes 101 – How to choose a fluorescent probe for your imaging experiment.
Lina El Hajji talks us through the optimal photophysical properties & weighs up the pros & cons of FPs versus small molecule dyes & the options in between!
focalplane.biologists.com/2024/12/14/p...

Great lecture from Prof. Neefjes in his visit to the Winter Lab!

I was looking for a starter pack for #WomenInSTEM working on #CellBiology and didn't find any - so I created one. And since I am hoping to not misgender anyone I made it #FLINTA (fingers crossed). Let me know if you want to be added!

go.bsky.app/5fp61o6

Microscopy Training Grants Supporting microscopy training for early-career researchers in cell biology Apply for a grant of up to £1000 Journal of Cell Science FocalPlane

Are you an #ECR who would like to attend a #microscopy or #bioimageanalysis training course in the next ~6 months but don't have sufficient funding, check to see if you are eligible for our JCS-FocalPlane Training Grant.

Next deadline: 22 November 2024

www.biologists.com/grants/jcs-f...

Q: What's wrong with these plots?

A: Pooling cell-level measurements across multiple runs conceals experimental reproducibility.

Read more in JCB (with @fritzlaylin.bsky.social @katrinavelle.bsky.social & Dyche Mullins): rupress.org/jcb/article/...

Haploinsufficiency of lysosomal enzyme genes in Alzheimers disease There is growing evidence suggesting that the lysosome or lysosome dysfunction is associated with Alzheimers disease (AD). Pathway analysis of post mortem brain-derived proteomic data from AD patients...

www.biorxiv.org/content/10.1...

Here are some free/open-source sites for the preparation of scientific illustrations, including icon libraries: bioicons.com, scidraw.io, bioart.niaid.nih.gov, inkscape.org, biogdp.com, reactome.org/icon-lib

Barely started on Twitter but seems like the real fun is here!