Patrick Murphy

The “Ins and Outs and What-Abouts” of H2A.Z: A tribute to C. David Allis - Journal of Biological Chemistry www.jbc.org/article/S002...

Check out the new chalk art outside the lab at Cornell!

@zebrafishrock.bsky.social could you please broadcast this?

TransTag enables simple and efficient transgene mapping in zebrafish via tagmentation Meng et al. develop a method called TransTag for simple and efficient mapping of Tol2-based transgenes in zebrafish. TransTag robustly identifies transgene insertion sites and offers an alignment-free...

Please RT: Published today, a super fun collaboration w/ @fanjumeng.bsky.social and Marnie Halpern's lab to establish methods for transgene mapping in zebrafish - TransTag enables simple and efficient transgene mapping in zebrafish via tagmentation: Cell Reports Methods www.cell.com/cell-reports...

TransTag enables simple and efficient transgene mapping in zebrafish via tagmentation Meng et al. develop a method called TransTag for simple and efficient mapping of Tol2-based transgenes in zebrafish. TransTag robustly identifies transgene insertion sites and offers an alignment-free...

TransTag enables simple and efficient transgene mapping in zebrafish via tagmentation: Cell Reports Methods www.cell.com/cell-reports...

Congrats Shan. It was wonderful to participate in the graduation ceremony with you! @urochester.bsky.social

Great event. Great trainees. Thank you!

Such an awesome day! It was wonderful to host Marisa Bartolomei @marisa-bartolomei.bsky.social as the Keynote speaker our annual Genetics Day symposium.

Congrats!!

Having an awesome time at the Tri-Repro meeting @pennmedicine.bsky.social @pennepigenetics.bsky.social with my good bud @themodzlab.bsky.social

I had a hypothesis that vitamin D prevents cancer. I worked on this for > 10 years, had 3 NIH-funded grants, and published >30 papers on this topic. Based on our work and that of my esteemed colleagues, I concluded that the evidence does not support the hypothesis.

RFK Jr., we are not the same.

Had a wonderful time visiting the Genetics Department at the University of Georgia. Great food! Great conversation! Great company! and Excellent weather! Thanks for the invite @gollmg.bsky.social

A cropped screenshot of the Flashes app in the App Store

Congratulations to @flashes.blue for their official launch on the App Store 🎉🎉🎉

It’s an instagram alternative built on the AT protocol 🙌 all the visual scrolling with none of the Meta algorithm

Congrats Brandon! So proud of your accomplishments.

We all know the story of Henrietta Lacks, but there are countless other examples when biomedical research advanced with a little regard for morals or ethics - often at the expense of poor, disadvantaged, or minority individuals. This book has been enlightening. A good read for current scientists.

CONGRATS to all!!

Now that I have read this paper, my lab has new findings based on CUT&Tag data that challenge the traditional view of euchromatic and repressive factors. I have started doubting all previous ChIPseq-based conclusions for many targets we are interested in. Thanks for publishing the preprint

CUT&Tag Identifies Repetitive Genomic Loci that are Excluded from ChIP Assays https://www.biorxiv.org/content/10.1101/2025.02.03.636299v1

Very interesting another reason to stop doing Chip seq and do cut&tag. Looking forward to reading it

Thanks so much Ed!!

... Finally, Brandon purified proteins that would otherwise be lost in the pellet in ChIP-Seq, and identified several interesting factors, such as BRD4, NSD2, and RUNX1, (which co-precipitated with repetitive heterochromatin) suggesting these factors might bind and regulate silenced chromatin... 5/5

... Brandon then demonstrated that in situ chromatin fragmentation techniques, like CUT&Tag or CUT&Run, can overcome this problem, allowing him to characterize H3K9me3 enrichment over evolutionarily young repetitive elements.... 4/n

... He goes on to demonstrate that these biases are due to heterochromatin begin resistant to sonication, relegating some genomic loci to the insoluble pellet, meaning a good amount of heterochromatin cannot be pulled down w/ antibodies... similar to prior results from Ken Zaret's lab... 3/n

... Brandon, as shared student between my lab and @mitchelloconnell.bsky.social, has identified major biases in ChIP-Seq datasets, which prohibit the study of heterochromatic loci and repetitive elements in several mammalian cell types... 2/n

PREPRINT! Park et al. makes the case that we may be misunderstanding heterochromatin for past 30 years due to ChIP-Seq biases... 1/n

We did a thing... We purified native transcriptional complexes from Drosophila embryos, obtaining +/- stalk Pol II elongation complexes, native nucleosomes, as well as a nucleosome elongation complex. For more details please check out: doi.org/10.1101/2025...

Here’s why we need to study & fund developmental biology: to understand, prevent & treat birth defects. Infants can’t speak, so we must be their advocates

WTF!!! This is a for real disaster!! He needs to be stopped. We can’t have this anti-science lunatic leading HHS.

🤦‍♂️ shit.