Himanshu (He/Him)

Is it possible for DNA to lag a little behind if the DpnI digested product was directly loaded onto the gel? I am expecting 5.5 kb bands, these are almost 5.8 if not 6. The plasmids were at 5.5 kb before I digested them.

What an incredible read! Seems weird to read something that was written a millennium ago.

In Early-Phase Pancreatic Cancer Clinical Trial, Investigational mRNA Vaccine Induces Sustained Immune Activity in Small Patient Group Learn how MSK researchers are deploying mRNA vaccines against pancreatic cancer.

I wonder if spreading extremely encouraging experimental results on helping treat pancreatic cancer with *gasp* mRNA vaccines may help people who have villainized them come around. This is a good press release on the study and links to the Nature paper. www.mskcc.org/news/can-mrn...

NIH ban on renewing senior scientists adds to assaults on its in-house research Policy follows firings of tenure-track scientists and suspension of training programs

What a concerning news! :(

www.science.org/content/arti...

Tears and panic as immediate impact of Trump's cuts felt by millions in South Africa — Sky News South Africa is home to one of the world's worst HIV/AIDS epidemics. At least 8.5 million people here are living with HIV - and now their treatment is in doubt thanks to Trump's executive order to fre...

apple.news/ARpLnggnJRYG...

Biologists discovered how people and horses get infected by hendra:
- Deforestation
- Bats starve
- Bats sicken, shed hendra virus
- People and horses get sick

Both are DH5alpha cells, picked two colonies off the plate and such a stark difference in colour.

Opinion | A Week Inside One of Northern Gaza’s Last Hospitals Most of the medical staff at Kamal Adwan Hospital is gone. One of the last doctors working there shares his story.

'We have called upon the world for protection for over 50 days but unfortunately there has been no response. I’m confounded by this world that claims to believe in humanity and democracy but does not respond. Not even the World Health Organization has any protection here.'

Trans athlete study concludes blanket bans in sports are a mistake The lead researcher of a landmark IOC-funded study looking at strength, power and aerobic capacity of trans athletes tells Outsports that sports federations should consider its findings carefully.

Study of trans athletes concludes blanket sports bans are a mistake.

The lead researcher of a landmark IOC-funded study looking at strength, power & aerobic capacity of trans athletes says “sports federations should consider its findings carefully.”

www.outsports.com/2024/4/12/24...

Time to head back to my gels.

Currently on the department retreat and tell me how do I go back to the lab when I know a view like this exists?

Thankyou, that does give a direction.

No, these are several different proteins with a single amino acid mutation. But yes, all lanes are cell lysates.

Stuck in protein expression. The proteins from lane 4 onwards should be 48kDa and yet they fall between 33-43kDa. Initially I thought it must have been some contamination so I did another transformation with fresh plasmid but still the same thing.

Variable effects of transient Wolbachia infections on alphaviruses in Aedes aegypti Author summary In recent years, wild populations of Aedes aegypti mosquitoes in over a dozen countries have been deliberately infected with Wolbachia pipientis (“Wolbachia”); an intracellular bacteriu...

🦟New study out of @vectorgen.bsky.social's lab finds #Wolbachia in Aedes aegypti can both help and hinder virus control, depending on the strain.

This highlights the need for more research to unlock Wolbachia's full potential against mosquito-borne diseases.

journals.plos.org/plosntds/art...

Currently struggling with protein induction and this image came on my timeline on that app we don't talk about.

Graph demonstrating rates of vaccine preventable illness decreasing over time

Vaccination saves lives. Period.

She looks like Ophelia here. Doesn't she?

Screenshot of anime Frieren Beyond Journey's End

If you are looking for a magical experience, may I introduce you to the anime Frieren Beyond Journey's End. It has beautiful visuals and music, intriguing lore, well-written characters, and masterful story telling. 100% recommend. One of the best, if not the best, anime of recent years.

Maybe they are purifying a protein and need the cold temperature

Thankyou so much 💗

Hi, can anyone direct me to a structural biology and/or structural and molecular virology starter packs?

Reminder: 1) there is no algorithm on here, if you found something interesting, make sure to repost, not just like. 2) repost your own post after 8 hrs so that people from different time zones can see.

Watching everyone move here is so motivating.

I can't believe we've been going without a functioning social media site for two years.

Wait, people have already started doing that here?

obviously the most recent explosion in growth is linked to the election but the enthusiastic level of engagement makes me think people are pretty hungry to interact with users who act like people instead of bots

Good news: Twitter is collapsing.

And this chart doesn't even show the last two weeks—which have seen the largest exodus in site history.

There are reports from Newsweek and others that Twitter may merge with Truth Social.

If that happens, the last collapse of Twitter may happen almost overnight.

I can't be the same person who used to be scared of running a SDS-PAGE gel. Look at me now, running several gels at once.