Clemens

Is today the day for phosphoramidite chemistry and/or next-gen sequencing??? #nobel #chemistry

08.10.2025 05:09 — 👍 0    🔁 0    💬 1    📌 0

Was great seeing you again :)

16.09.2025 16:47 — 👍 1    🔁 0    💬 0    📌 0

Engineering fluoroacetate dehalogenase by growth-based selections to degrade non-natural organofluorides The widespread use of organofluorides in modern society has inadvertently led to the bioaccumulation of harmful pollutants, most prominently per- and polyfluorinated alkyl substances (PFAS). In princi...

Preprint alert! With #PFAS contamination becoming an environmental crisis, we dared to engineer natural defluorinases towards degradation of (un)natural organofluorides. How? Check out our robust growth-based selection platform here: www.biorxiv.org/content/10.1... thanks to @mayerlab.bsky.social !

17.07.2025 09:38 — 👍 10    🔁 5    💬 0    📌 0

Overall, our study presents the first large-scale engineering campaign of FAcDs and introduces an operationally simple selection platform that paves the way toward adapting these enzymes for the sustainable degradation of contaminating organofluorides. (5/5)

17.07.2025 08:51 — 👍 0    🔁 0    💬 0    📌 0

Forcing populations of bacteria expressing tens of thousands of distinct FAcD-variants to utilize various organofluorides as sole carbon source, we elicited a panel of FAcD variants with improved activities and drastically altered substrate profiles. (4/5)

17.07.2025 08:51 — 👍 0    🔁 0    💬 1    📌 0

To expand the substrate scope of FAcDs for non-natural organofluorides, we designed and applied a robust growth-based selection, in which the FAcD-catalyzed C—F bond cleavage of synthetic organofluorides generates metabolizable C-sources. (3/5)

17.07.2025 08:51 — 👍 0    🔁 0    💬 1    📌 0

While tailored biocatalysts able to cleave C—F bonds represent an attractive strategy to remediate organofluorides, Nature is largely agnostic to C—F bonds. Fluoroacetate dehalogenases (FAcDs) stands out by promoting the hydrolysis of single C—F bonds in fluoroacetate at high turnover rates. (2/5)

17.07.2025 08:51 — 👍 0    🔁 0    💬 1    📌 0

Engineering fluoroacetate dehalogenase by growth-based selections to degrade non-natural organofluorides The widespread use of organofluorides in modern society has inadvertently led to the bioaccumulation of harmful pollutants, most prominently per- and polyfluorinated alkyl substances (PFAS). In princi...

With #PFAS and other organofluorides wreaking havoc, we set out to engineer natural defluorinases for man-made organofluorides. All credit goes to the amazing @suzannejansen.bsky.social, who has outdone herself in this project! (1/5)
www.biorxiv.org/content/10.1...

17.07.2025 08:51 — 👍 9    🔁 4    💬 1    📌 1

Original post on mstdn.science

In our recent publication, we describe a systematic framework leveraging the documentation of the quantum chemistry suite ORCA for developing large language models that are capable of providing input files based on user prompts with the help of prompt engineering, retrieval-augmented generation […]

11.02.2025 18:22 — 👍 11    🔁 5    💬 0    📌 0

Two weeks ago, we had a great group outing, including a city tour through Utrecht, a Mario Kart Wii tournament, and some Rock Band Wii fun! I am truly privileged to work with such a group of talented, dedicated, and smart individuals.

11.02.2025 19:55 — 👍 6    🔁 0    💬 0    📌 1

Hello world, hello BlueSky 🙂

11.02.2025 16:09 — 👍 14    🔁 4    💬 2    📌 0